qPCR / RealTime PCR

Fast and Reliable qPCR from Plant SamplesThe InhibiCore Rapid Plant PCR MasterMix (5X) is specifically designed for fast, reliable, and highly sensitive qPCR from plant material. Plant samples often contain PCR inhibitors such as polyphenols, polysaccharides, and other secondary metabolites that can negatively affect amplification efficiency. Engineered for exceptional inhibitor tolerance, the InhibiCore Rapid Plant PCR MasterMix delivers robust and reproducible amplification even from challenging plant-derived samples.The included modified HotStart Taq DNA Polymerase provides outstanding specificity and sensitivity while minimizing non-specific amplification. This ensures reliable and accurate results, even in demanding applications. The master mix contains an optimized PCR buffer, dNTPs, and MgCl₂, enabling convenient and streamlined reaction setup. Since the HotStart polymerase remains inactive at room temperature, no cold setup is required, simplifying workflow and reducing the risk of non-specific amplification.The master mix is also lyo-ready, making it an excellent choice for the development of stable, room-temperature assay formats. In addition, the incorporation of dUTP enables effective carry-over contamination control when used together with UDG (Uracil-DNA Glycosylase).For rapid direct PCR workflows, we recomment the InhibiCore Rapid Plant DNA Q-Extraction PCR Kit (M3252), providing a fast and efficient sample preparation method that integrates seamlessly with downstream qPCR applications.Key BenefitsSpecifically optimized for plant samples and inhibitor-rich plant matricesExceptional inhibitor tolerance for reliable amplificationHigh sensitivity and specificity for accurate target detectionConvenient room-temperature setup with HotStart technologyLyo-ready and dUTP-enabled contamination control capabilityTypical ApplicationsPlant genotypingPlant pathogen detectionAgricultural and plant researchGMO testingSeed quality controlDirect PCR from plant material Applications examples:1. Sensitive qPCR analysis of chlorophyll gene targets from wheat lysatesReliable and sensitive detection of chlorophyll gene targets from plant lysates was demonstrated using qPCR with the InhibiCore Rapid Plant PCR MasterMix (5X) and fluorescent dye chemistry. A serial dilution of wheat lysate (10% to 0.001%), prepared with Plant DNA Q-Extraction Solution (M3230) was used to evaluate sensitivity and inhibitor tolerance.Successful amplification was achieved across all dilutions, confirming strong robustness against plant-derived inhibitors even at high lysate concentrations. A single, specific melt curve peak confirmed assay specificity with no non-specific amplification. Samples were prepared using the rapid 8-minute Plant DNA Q-Extraction Solution protocol, enabling fast DNA preparation without conventional purification steps. Figure 1: qPCR data by wheat lysate qPCR with InhibiCore Rapid Plant PCR MasterMix (5X).The figure shows qPCR amplification curves and melting curve analysis of a wheat lysate dilution series (10% to 0.001%) and a no-template control (NTC) using InhibiCore Rapid Plant PCR MasterMix (5X) and 1xGreenDye. Clear, concentration-dependent amplification is observed in the FAM channel, with reduced signal intensity at 10% lysate due to matrix effects. Melting curve analysis reveals a single specific peak for all positive samples, confirming specific target amplification without non-specific products. 2. Benchmark of Direct PCR performance from 10% tomato lysateThe performance of the InhibiCore Rapid Plant PCR MasterMix (5X) was evaluated against three competitor master mixes for qPCR from 10% tomato lysate. Samples were prepared using the Plant DNA Q-Extraction Solution and analyzed by fluorescent dye detection in the FAM channel.The InhibiCore Master Mix showed successful amplification with the earliest Ct values, demonstrating superior efficiency and the highest inhibitor tolerance compared to all tested competitors. Two competitor systems also produced amplification, while two showed strong inhibition with no detectable signals. Specific amplification was confirmed by a single, distinct melt curve peak with no evidence of non-specific products or primer-dimer formation.Figure 2: Plant direct PCR from plant samples Benchmark - inhibitor effect from Tomato lysateDirect PCR was performed using a cycling protocol of 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 sec and 60 °C for 30 sec, with subsequent melt curve analysis. InhibiCore Rapid Plant PCR MasterMix (5X) and Competitor N showed successful amplification, while Competitor T and Competitor Q were strongly inhibited and did not generate reliable amplification curves. InhibiCore Rapid Plant PCR MasterMix (5X) exhibited the earliest Ct value among all tested systems, indicating the highest tolerance to plant-derived PCR inhibitors and superior performance in crude tomato lysates.

ProbeMasterMix without ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Multiplex PCR: Applications at Genaxxon and at customers site have shown that the qPCR Probe Mastermix can be used for the simultaneous detection of up to four DNA targets in the same PCR reaction. For further details please refer to the product manual or contact us: info@genaxxon.com. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMasterMix without ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

ProbeMasterMix low ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X master mix is ready-to-use and contains optimised amounts of all ingredients. Multiplex PCR: Applications at Genaxxon and at customers site have shown that the qPCR Probe Mastermix can be used for the simultaneous detection of up to four DNA targets in the same PCR reaction. For further details please refer to the product manual or contact us: info@genaxxon.com. Advantages of the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP 50nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMastermix Low ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix high ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye 50nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

ProbeMastermix High ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X Mastermix is ready-to-use and contains optimised amounts of all ingredients. Multiplex PCR: Applications at Genaxxon and at customers site have shown that the qPCR Probe Mastermix can be used for the simultaneous detection of up to four DNA targets in the same PCR reaction. For further details please refer to the product manual or contact us: info@genaxxon.com. Advantages of the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix high ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP 500nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMastermix High ROX optimised for realtime PCR assays in block systems that contains all components to perform quantitative PCR with the exception of primer and template DNA. This 2X PCR master mix is ready-to-use and contains optimised amounts of all ingredients. Advantages of the chemically modified SuperHot Taq polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperatur No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix high ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye 500nM ROX optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.

ProbeMasterMix FAST without ROX is optimised for fast qPCR assays with probes in block systems. The master mix is optimized for fast PCR with short denaturation 2-step cycles. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the Hotstart Taq polymerase used in the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturation of not more than 2 minutes. optimized for 2-step PCR protocols. No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon Hotstart Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The hotstart formulation inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMasterMix FAST without ROX is optimised for fast qPCR assays without probes in block systems. The master mix is optimized for fast PCR with short denaturation 2-step cycles. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the HotStart Taq DNA polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturing time of not more than 2 minutes No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix without ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: Hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) Stability at +2°C to +8°C (refrigerator): minimum of 8 months. This Mastermix is specially suited for the following instruments: BioRad CFX96 Touch™, CFX384 Touch™, CFX Connect™, DNA Engine Opticon® 2, Chromo4™, iCycler iQ™ and My iQ™ , Roche LightCycler® 480, LightCycler® 1536, LightCycler® Nano, LightCycler® 96 and QuantStudio™ instruments, Thermo Scientific™ PikoReal™, Cepheid SmartCycler®, Bio Molecular Systems Mic qPCR cycler, Qiagen Rotor Gene Q, Rotor Gene 6000, MyGo Mini and MyGo Pro. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

ProbeMasterMix FAST Low ROX with 50nM ROX is optimised for fast qPCR assays with probes in block systems. The master mix is optimized for fast qPCR with short denaturation 2-step cycles. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA Advantages of the Hotstart Taq polymerase used in the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturation of not more than 2 minutes. optimized for 2-step PCR protocols. No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon Hotstart Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The hotstart formulation inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix with 50nM ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP optimized reaction buffer 50nM ROX as internal passive reference stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMasterMix FAST Low ROX with 50nM ROX is optimised for realtime PCR assays in block systems. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the HotStart Taq DNA polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturing time of not more than 2 minutes No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master Low ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: Hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets 50nM internal passive reference fluorescence dye the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 7500, 7500 Fast and ViiA™ 7, QuantStudio™ instruments, Agilent Mx3000P™, Mx3005P™, Mx4000™ and AriaMx. Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.

ProbeMasterMix FAST High ROX with 500nM ROX optimised for fast realtime PCR assays in block systems. This master mix is ready-to-use and contains all components for a successful and reliable quantitative PCR with the exception of primer and template DNA. Advantages of the Hotstart Taq polymerase used in the Genaxxon ProbeMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturation of not more than 2 minutes. optimized for 2-step PCR protocols. No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon Hotstart Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The hotstart formulation inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Probe qPCR master mix High ROX with 500nM ROX contains all the necessary components in an optimized composition to carry out quantitative PCR. hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP optimized reaction buffer 500nM ROX as internal reference stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMasterMix FAST High ROX with 500nM ROX is optimised for realtime PCR assays in block systems. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the HotStart Taq DNA polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturing time of not more than 2 minutes No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR MasterMix High ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: Hotstart Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

GreenMasterMix FAST Blue with 500nM ROX is optimised for realtime PCR assays in block systems. This 2X Mastermix is ready-to-use and contains all components for a successful and reliable qPCR with the exception of primer and template DNA. Advantages of the HotStart Taq DNA polymerase used in the Genaxxon GreenMasterMix: Hotstart technology enables setup of PCR mixture at room temperature. short initial denaturing time of not more than 2 minutes No pipetting on ice necessary anymore No immediate further processing (PCR) necessary. The pipetted pre-PCR mixtures can be left at RT for up to 3 days. Amplification of GC-rich templates High yields No Primer dimers The Genaxxon SuperHot Taq DNA polymerase offers convenience and practicality, without compromising efficiency or specificity: 1. The chemical modification inhibits the polymerase, which prevents false priming at all temperatures tested, allowing for PCR reactions to be set up at RT (20°C to 25°C), and 2. "High mix stability allows pre-assemby of the plate in advance", which means PCR reactions can be prepared and stored at RT for up to three days before they are run, without affecting the results. The Green qPCR master mix Blue with 500nM ROX contains all the necessary components in an optimized composition to carry out quantitative PCR: chemically modified Taq DNA Polymerase dATP, dCTP, dGTP, dTTP intercalating green fluorescent dye optimized reaction buffer stabilizers and enhancers to enable even amplification of low copy number targets the small aliquote size of 1.25mL simplifies handling and storage (less freeze-thaw cycles per aliquote) This Mastermix is specially suited for the following instruments: Applied Biosystems® 5700, 7000, 7300, 7700, 7900, 7900 HT, Eppendorf Realplex4, StepOne™ and StepOnePlus™. Other realtime PCR master mixes from Genaxxon are: M3023 - GreenMastermix No ROX >, M3045 - ProbeMastermix No ROX >, M3011 - GreenMastermix Low ROX >, M3031 - ProbeMastermix Low ROX >, M3052 - GreenMastermix High ROX >, M3010 - ProbeMastermix High ROX >.

Multiplex PCR is a method that enables amplification of two or more amplicons simultaneously in a single reaction tube/reaction. It is widely used in genotyping and different areas of DNA testing in research, forensic and diagnostic laboratories. Our Multiplex HS Mastermix (2X) is an optimized ready-to-use mixture for probe-based assays such as TaqMan®, Beacons and MGBs. It contains a modified fast HotStart Taq DNA Polymerase, dNTPs and MgCl₂ combined in an optimized buffer system for realtime PCR / qPCR applications except primers, probe and template DNA / cDNA.  The HotStart Taq Polymerase is based on the standard Taq DNA polymerase from Genaxxon inactivated by an specific antibody against Taq DNA polymerase which is activated by heat treatment. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity, increased sensitivity and greater yields when compared to standard DNA polymerases, making this enzyme especially well-suited for multiplex PCR. - Amplification of multiple targets in a single tube- All-in-one master mix for convenient multiplexing- High specificity, sensitivity and product yield- Easy reaction setup at room temperature The Multiplex HS Master mix 2X is shipped in aliquots of 1mL. Our Standard Agarose LE > and especially our high resolution Agarose Tiny > are ideally suited for the subsequent electrophoresis analysis. Other realtime master mixes for your realtime PCR experiments can be found here >. Examples of Multiplex applications: F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002. Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.

Lyo Ready qPCR MastermixA powerful, all-in-one solution for multiplex real-time PCR, optimized for high sensitivity, crude sample compatibility, and lyophilization. This highly concentrated 5x qPCR Master Mix is specifically designed for multiplex real-time PCR applications. It supports up to 30 targets in a single reaction, allows direct amplification from crude samples like blood or swabs (no extraction needed), and ensures high sensitivity with more space for primers and probes. Fully lyophilization-ready, it’s ideal for kit manufacturing, ambient shipping, and long-term storage without refrigeration.Key Benefits:Sensitive – More room for what mattersWith its 5x concentration, this Master Mix maximizes free volume for target-specific primers and probes. Ideal for high-level multiplexing without sacrificing sensitivity.Robust – Reliable results across targets5x qPCR Multiplex MasterMix ensures consistent, uniform amplification — even in complex multiplex panels.Fast Time to Result – No extraction neededWorks directly on crude samples like blood and swabs, eliminating the need for time-consuming DNA extraction steps.Specific – Precision built-inEngineered Taq DNA polymerase with enhanced room temperature stability and aptamer-based hot-start technology prevents non-specific amplification and enables a rapid start.Lyo Ready – Built for freeze-dryingFormulated with all necessary excipients for lyophilization. Can be freeze-dried in-house or by us. Once dried, it supports ambient storage and shipping — ideal for kit manufacturing and field use. Application notes:Learn more about how to use this product in practice – download the application note here.Read now in our blog why the 5x qPCR Multiplex MasterMix is the optimal choice for your multiplex qPCR. Our Standard Agarose LE and especially our high resolution Agarose Tiny are ideally suited for the subsequent electrophoresis analysis. Other realtime master mixes for your realtime PCR experiments can be found here .Examples of Multiplex applications: F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002. Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.

Lyophilized 5X Multiplex PCR Mastermix for robust PCR with all components for rapid, sensitive and reproducible quantification of DNA. The optimized DNA polymerase and an optimized buffer including our ultrapure dNTPs are key components of the ready to use mix. A hot-start formulation of the included DNA polymerase prevents false amplification during the reaction setup. Our 5X qPCR master mix will function with a wide range of templates including human-, mammal-, and plant-derived samples. Features:- 5-time concentrate for more variability in primer- and probe volumes- amplification of different targets in a single PCR tube (tested for up to 4 targets).- the hot-start formulation of the included DNA polymerase prevents false amplification during the reaction setup- the optimized buffer includes our ultrapure dNTPs Our new lyophilized Multiplex master mix for fast and easy multiplexing minimizes the need for optimization and makes the development of multiplex PCR assays fast and easy. Our new 5-fold multiplex mastermix minimizes the need for optimization and therefore makes the development and establishment of multiplex PCR easier and faster.  Stability- the lyophilized MasterMix is stable for 3 years, if stored at -20°C.- the lyophilized MasterMix is stable for at least 12 months, if stored after delivery at +15°C to+30°C.- the reconstituted MasterMix is stable for 6 months, if stored at -20°C. Read now in our blog why the 5x qPCR Multiplex MasterMix is the optimal choice for your multiplex qPCR. Our Standard Agarose LE > and especially our high resolution Agarose Tiny > are ideally suited for the subsequent electrophoresis analysis. Other realtime master mixes for your realtime PCR experiments can be found here >. Examples of Multiplex applications:F. Javier Pérez-Pérez and Nancy D. Hanson, Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR, J. Clin. Microbiol. June 2002 vol. 40 no. 6 2153-2162. doi: 10.1128/JCM.40.6.2153-2162.2002. Tamara B. Souzaa, Diego M. Lozerb, Sônia M. S. Kitagawab, Liliana C. Spanob, Neusa P. Silvac and Isabel C. A. Scaletskya, Real-Time Multiplex PCR Assay and Melting Curve Analysis for Identifying Diarrheagenic Escherichia coli. J. Clin. Microbiol. March 2013 vol. 51 no. 3 1031-1033, doi: 10.1128/JCM.02478-12.

Lyophilized (freeze-dried) temperature stable and pre-mixed Hotstart master mix with all necessary components for probe realtime PCR (optimized buffer, dNTPs, Hotstart Taq) for difficult to amplify templates, qPCR and Multiplex applications. No cooling/cooling chain necessary! Our LyoMix and all ingredients are unopened stable at ambient temperature for at least 24 months! Our lyophilized qPCR master mix is also available as LyoBalls pre-portioned in PCR tube strips or PCR plates (M3069 > - 0.1mL tubes or M3070 > - 0.2mL tubes)! Our Hotstart Polymerase guarantees that during setup and the first PCR cycle the enzyme is not active and misprimed primers are not extended. As a result specificity and efficiency are increased by far compared to standard Taq DNA polymerase. Additionally, difficult targets with high GC-content can be amplified. The higher sensitivity improves multiplex PCR results.This lyophilized master mix in powder form is the perfect choice for a fast reaction setup that reduces the time required for pipetting and the possibility for pipetting errors. Just add PCR-grade water, template and primers to reconstitute the master mix and you are all set! Lyophilized realtime PCR master mix:• Hotstart Polymerase• increased specificity• increased sensitivity• for difficult to amplify templates with high GC content• for Multiplex applications• for qPCR• ready-to-useThis lyophilized Hotstart master mix is delivered in powder form that can be easily dispensed. With our high quality dNTPs as Set > (M3015.4100 and M3015.0250) or Mix (M3016.1010) > or our DNA Ladders > and our favourable standard agarose (M3044) > we can offer additional products for your PCR.