Fast and Easy Genotyping Without DNA Extraction

Customized Direct Genotyping PCR Kits – Tailored to Your Research Needs Our customized Direct Genotyping PCR Kits offer rapid, real-time genotyping without the need for prior DNA isolation. By providing the specific SNP identifiers (rs-numbers) and species details (human, animal, or other), you can request a personalized kit designed to precisely match your requirements. Upon confirmation of your order, we will develop and deliver your customized genotyping kit within approximately 4-8 weeks. Multiplex assays (2-4 targets simultaneously) can also be accommodated upon request. These multiplex reactions utilize fluorescence channels such as FAM, HEX, ROX, and Cy5 allowing simultaneous detection of multiple genetic markers. Please ensure your real-time PCR cycler is compatible with these fluorescence wavelengths and supports necessary color compensation functionalities. Key features and benefits No DNA Extraction Required: Perform direct PCR from crude samples like saliva, oral swabs, blood, or urine Real-Time Genotyping: Efficient, rapid results without the delays associated with DNA isolation. Hot-Start Technology: Allows reaction setup at room temperature, improving workflow convenience. Universal Compatibility: Designed for compatibility with all common real-time PCR cyclers. Reliable Fluorescence Detection: Accurate genotype determination using established fluorescence channels. Eco-Friendly Shipping: Room-temperature stability eliminates the necessity for cold-chain logistics. Ordering Information Customized assays are priced as singleplex assays. Multiplex assays are available upon inquiry, with pricing determined individually. Upfront payment is required for customized orders. How the Technology Works Real-time genotyping kits distinguish genetic variants based on melting curve analysis. Differences in melting peaks clearly identify homozygous wild-type, heterozygous, and homozygous mutant genotypes. Please note that minor shifts in melting temperatures may occur due to variations in sample composition (e.g., salt concentration or pH levels). Contact us directly with your specific SNP (rs-number) and species details to start creating your customized Direct Genotyping PCR Kit. More information? Watch our explainer video:

rs1799963, Prothrombin G20210A Mutation - Fast, Real-Time Detection Without DNA Isolation Designed for the accurate identification of Prothrombin SNP G20210A, rs1799963, this kit supports the detection of a genetic variation strongly associated with an increased risk of thromboembolic events, such as deep vein thrombosis (DVT) and pulmonary embolism (PE). Early detection helps identify individuals who may benefit from preventive measures to reduce the risk of thrombotic complications. The Genaxxon Direct Factor II Genotyping PCR Kit enables real-time genotyping without the need for prior DNA isolation, allowing for a faster and more cost-effective workflow.  Key Features & BenefitsNo DNA extraction required – Perform direct PCR from crude samples such as saliva, oral swabs, blood, and urine.Genotyping in real-time – Rapid and efficient workflow without time-consuming DNA isolation.Hot-start formulation – Enables convenient room-temperature reaction setup.Platform-independent – Compatible with all modern real-time PCR cyclers.Reliable fluorescence-based detection – FAM channel measurement ensures accurate genotype determination.Eco-friendly shipping – Stable at room temperature, reducing the need for cold-chain transport.The Genaxxon Direct Factor II Genotyping PCR Kit is the ideal solution for researchers and clinicians seeking a fast, sensitive, and reliable method for thrombosis risk assessment—saving both time and money. How does it work?Shown is exemplary genotyping data from two different blood samples.The green melting curve originates from an EDTA blood sample and shows a single melting peak at 66 °C. This clearly indicates the presence of a homozygous wild-type genotype.In contrast, the blue melting curve from another EDTA blood sample displays two distinct melting peaks—one at the same temperature as the green wild-type sample (66 °C), and a second at a significantly lower temperature (54 °C). The presence of two peaks is characteristic of a heterozygous genotype, indicating that two different alleles are present in the sample.A single melting peak at the lower temperature would, in turn, indicate the presence of a homozygous mutant genotype.Please note: minor shifts in melting temperatures can occur due to variations in salt concentration and pH in crude samples, which may influence the melting behavior slightly. More information? Watch our explainer video:

rs6025, Factor V Leiden G1691A, R506Q Mutation - Fast, Real-Time Genotyping without DNA Isolation Designed for the precise identification of Factor V Leiden G1691A (rs6025), this kit supports the detection of the most common inherited form of thrombophilia, which is associated with resistance to activated protein C. Individuals carrying this mutation have an elevated risk of venous thromboembolism, making identification crucial for clinical management and risk assessment. The Genaxxon Direct Factor V Leiden Genotyping PCR Kit enables real-time genotyping without the need for prior DNA isolation, allowing for a faster and more cost-effective workflow. Key Features & BenefitsNo DNA extraction required – Perform direct PCR from crude samples such as saliva, oral swabs, blood, and urine.Genotyping in real-time – Rapid and efficient workflow without time-consuming DNA isolation.Hot-start formulation – Enables convenient room-temperature reaction setup.Platform-independent – Compatible with all modern real-time PCR cyclers.Reliable fluorescence-based detection – FAM channel measurement ensures accurate genotype determination.Eco-friendly shipping – Stable at room temperature, reducing the need for cold-chain transport. The Genaxxon Direct Factor V Leiden Genotyping PCR Kit is the ideal solution for researchers and clinicians seeking a fast, precise, and reliable method for identifying the most common inherited form of thrombophilia – allowing for early detection and risk assessment of venous thromboembolism.How does it work?Shown is exemplary genotyping data from two different blood samples.The red melting curve originates from an EDTA blood sample and shows a single melting peak at 66°C. This clearly indicates the presence of a homozygous wild-type genotype.In contrast, the green melting curve from another EDTA blood sample displays two distinct melting peaks—one at the same temperature as the yellow wild-type sample (66°C), and a second at a significantly lower temperature (54°C). The presence of two peaks is characteristic of a heterozygous genotype, indicating that two different alleles are present in the sample.A single melting peak at the lower temperature would, in turn, indicate the presence of a homozygous mutant genotype.Please note: minor shifts in melting temperatures can occur due to variations in salt concentration and pH in crude samples, which may influence the melting behavior slightly.Picture 1: More information? Watch our explainer video:

rs1800562, HFE C282Y Mutation - Fast, Real-Time Detection Without DNA Isolation Designed for the precise identification of the HFE1 SNP G845A (rs1800562), this kit supports the detection of a genetic variant strongly linked to hereditary hemochromatosis, an iron overload disorder characterized by excessive iron absorption. Early identification allows proactive management to prevent organ damage related to iron accumulation. The Genaxxon Direct HFE1 Genotyping PCR Kit enables real-time genotyping without the need for prior DNA isolation, allowing for a faster and more cost-effective workflow. Key Features & BenefitsNo DNA extraction required – Perform direct PCR from crude samples such as saliva, oral swabs, blood, and urine.Genotyping in real-time – Rapid and efficient workflow without time-consuming DNA isolation.Hot-start formulation – Enables convenient room-temperature reaction setup.Platform-independent – Compatible with all modern real-time PCR cyclers.Reliable fluorescence-based detection – FAM channel measurement ensures accurate genotype determination.Eco-friendly shipping – Stable at room temperature, reducing the need for cold-chain transport.The Genaxxon Direct HFE1 Genotyping PCR Kit is the ideal solution for researchers and clinicians seeking a fast, sensitive, and reliable method for identifying hereditary hemochromatosis – all while saving time and costs. How does it work?Shown is exemplary genotyping data from two different blood samples.The yellow melting curve originates from an EDTA blood sample and shows a single melting peak at 65°C. This clearly indicates the presence of a homozygous wild-type genotype.In contrast, the red melting curve from another EDTA blood sample displays two distinct melting peaks—one at the same temperature as the green wild-type sample (65°C), and a second at a significantly lower temperature (55°C). The presence of two peaks is characteristic of a heterozygous genotype, indicating that two different alleles are present in the sample.A single melting peak at the lower temperature would, blue curve, in turn, indicate the presence of a homozygous mutant genotype.Please note: minor shifts in melting temperatures can occur due to variations in salt concentration and pH in crude samples, which may influence the melting behavior slightly.Picture 1: More information? Watch our explainer video:

rs1799945, HFE H63D Mutation - Fast, Real-Time Detection Without DNA IsolationDesigned for the precise identification of the HFE2 SNP C187G (rs1799945), this kit supports the detection of a genetic variant associated with hereditary hemochromatosis, particularly when present alongside other genetic variants that impact iron metabolism and storage. Early identification allows for proactive management to monitor iron levels and prevent complications related to iron overload. The Genaxxon Direct HFE2 Genotyping PCR Kit enables real-time genotyping without the need for prior DNA isolation, allowing for a faster and more cost-effective workflow. Key Features & BenefitsNo DNA extraction required – Perform direct PCR from crude samples such as saliva, oral swabs, blood, and urine.Genotyping in real-time – Rapid and efficient workflow without time-consuming DNA isolation.Hot-start formulation – Enables convenient room-temperature reaction setup.Platform-independent – Compatible with all modern real-time PCR cyclers.Reliable fluorescence-based detection – FAM channel measurement ensures accurate genotype determination.Eco-friendly shipping – Stable at room temperature, reducing the need for cold-chain transport.The Genaxxon Direct HFE2 Genotyping PCR Kit is the ideal solution for researchers and clinicians seeking a fast, sensitive, and reliable method for identifying hereditary hemochromatosis – all while saving time and costs. How does it work?Shown is exemplary genotyping data from two different blood samples.The red melting curve originates from an EDTA blood sample and shows a single melting peak at 66°C. This clearly indicates the presence of a homozygous wild-type genotype.In contrast, the green melting curve from another EDTA blood sample displays a pronounced melting peak at a significantly lower temperature (55°C). A single melting peak at this lower temperature indicates a homozygous mutant genotype. Conversely, the presence of two peaks in a melting curve (at 66°C and at 55°C) would be characteristic of a heterozygous genotype, thus indicating the presence of two different alleles in the sample.Please note: minor shifts in melting temperatures can occur due to variations in salt concentration and pH in crude samples, which may influence the melting behavior slightly.Picture 1: More information? Watch our explainer video:

rs4988235, LCT C13910T Mutation - Fast, Real-Time Detection Without DNA Isolation Designed for the precise identification of the LCT SNP C13910T (rs4988235), this kit supports the detection of a genetic variant associated with lactose intolerance. Early detection enables targeted dietary recommendations and improves gastrointestinal health. The Genaxxon Direct Lactose Intolerance Genotyping PCR Kit enables real-time genotyping without the need for prior DNA isolation, allowing for a faster and more cost-effective workflow. Key Features & BenefitsNo DNA extraction required – Perform direct PCR from crude samples such as saliva, oral swabs, blood, and urine.Genotyping in real-time – Rapid and efficient workflow without time-consuming DNA isolation.Hot-start formulation – Enables convenient room-temperature reaction setup.Platform-independent – Compatible with all modern real-time PCR cyclers.Reliable fluorescence-based detection – FAM channel measurement ensures accurate genotype determination.Eco-friendly shipping – Stable at room temperature, reducing the need for cold-chain transport.The Genaxxon Direct HFE2 Genotyping PCR Kit is the ideal solution for researchers and clinicians seeking a fast, sensitive, and reliable method for identifying hereditary hemochromatosis – all while saving time and costs. How does it work?Shown is exemplary genotyping data from two different blood samples.The green melting curve originates from an EDTA blood sample and shows a single melting peak at 66 °C. This clearly indicates the presence of a homozygous wild-type genotype.In contrast, the blue melting curve from another EDTA blood sample displays two distinct melting peaks—one at the same temperature as the green wild-type sample (66 °C), and a second at a significantly lower temperature (54 °C). The presence of two peaks is characteristic of a heterozygous genotype, indicating that two different alleles are present in the sample.A single melting peak at the lower temperature would, in turn, indicate the presence of a homozygous mutant genotype.Please note: minor shifts in melting temperatures can occur due to variations in salt concentration and pH in crude samples, which may influence the melting behavior slightly. More information? Watch our explainer video:

rs1801131, MTHFR A1298C Mutation - Fast, Real-Time Detection Without DNA Isolation Designed for the precise identification of the MTHFR SNP A1298C (rs1801131), this kit supports the detection of a genetic variant linked to reduced activity of the MTHFR enzyme, which is crucial for folate metabolism and maintaining optimal homocysteine levels. Identifying carriers allows for targeted nutritional interventions to prevent related cardiovascular and neurological conditions. The Genaxxon Direct MTHFR1 Genotyping PCR Kit enables real-time genotyping without the need for prior DNA isolation, allowing for a faster and more cost-effective workflow. Key Features & BenefitsNo DNA extraction required – Perform direct PCR from crude samples such as saliva, oral swabs, blood, and urine.Genotyping in real-time – Rapid and efficient workflow without time-consuming DNA isolation.Hot-start formulation – Enables convenient room-temperature reaction setup.Platform-independent – Compatible with all modern real-time PCR cyclers.Reliable fluorescence-based detection – FAM channel measurement ensures accurate genotype determination.Eco-friendly shipping – Stable at room temperature, reducing the need for cold-chain transport.The Genaxxon Direct MTHFR1 Genotyping PCR Kit is the ideal solution for researchers and clinicians seeking a fast, sensitive, and reliable method for identifying MTHFR variants – while saving time and costs. How does it work?Shown is exemplary genotyping data from two different blood samples.The green melting curve originates from an EDTA blood sample and shows a single melting peak at 66 °C. This clearly indicates the presence of a homozygous wild-type genotype.In contrast, the blue melting curve from another EDTA blood sample displays two distinct melting peaks—one at the same temperature as the green wild-type sample (66 °C), and a second at a significantly lower temperature (54 °C). The presence of two peaks is characteristic of a heterozygous genotype, indicating that two different alleles are present in the sample.A single melting peak at the lower temperature would, in turn, indicate the presence of a homozygous mutant genotype.Please note: minor shifts in melting temperatures can occur due to variations in salt concentration and pH in crude samples, which may influence the melting behavior slightly. More information? Watch our explainer video:

rs1801133, MTHFR C677T Mutation - Fast, Real-Time Detection Without DNA Isolation Designed for the precise identification of the MTHFR2 SNP C677T (rs1801133), this kit supports the detection of a common genetic variant that significantly decreases the efficiency of the MTHFR enzyme, leading to elevated homocysteine levels and reduced folate availability. Early detection supports clinical decisions on nutritional supplementation and preventive care to reduce associated health risks. The Genaxxon Direct MTHFR2 Genotyping PCR Kit enables real-time genotyping without the need for prior DNA isolation, allowing for a faster and more cost-effective workflow. Key Features & BenefitsNo DNA extraction required – Perform direct PCR from crude samples such as saliva, oral swabs, blood, and urine.Genotyping in real-time – Rapid and efficient workflow without time-consuming DNA isolation.Hot-start formulation – Enables convenient room-temperature reaction setup.Platform-independent – Compatible with all modern real-time PCR cyclers.Reliable fluorescence-based detection – FAM channel measurement ensures accurate genotype determination.Eco-friendly shipping – Stable at room temperature, reducing the need for cold-chain transport.The Genaxxon Direct MTHFR2 Genotyping PCR Kit is the ideal solution for researchers and clinicians seeking a fast, sensitive, and reliable method for identifying MTHFR variants – while saving time and costs. How does it work?Shown is exemplary genotyping data from two different blood samples.The green melting curve originates from an EDTA blood sample and shows a single melting peak at 66 °C. This clearly indicates the presence of a homozygous wild-type genotype.In contrast, the blue melting curve from another EDTA blood sample displays two distinct melting peaks—one at the same temperature as the green wild-type sample (66 °C), and a second at a significantly lower temperature (54 °C). The presence of two peaks is characteristic of a heterozygous genotype, indicating that two different alleles are present in the sample.A single melting peak at the lower temperature would, in turn, indicate the presence of a homozygous mutant genotype.Please note: minor shifts in melting temperatures can occur due to variations in salt concentration and pH in crude samples, which may influence the melting behavior slightly. More information? Watch our explainer video:

Schnelle, Real-time Genotypisierung ohne DNA-Isolation The HLA-B27 Direct Duplex qPCR Detection Kit enables fast and reliable qualitative detection of the HLA-B27 allele using hydrolysis probe-based real-time PCR in a duplex format. The assay is optimized for direct use of EDTA whole blood and can alternatively be performed with purified genomic DNA. Simultaneous amplification of an HLA-B27 specific target and an internal control ensures high result reliability and minimizes the risk of false-negative outcomes. The kit is compatible with real-time PCR cyclers featuring FAM and ROX detection channels. The Genaxxon Direct HLA-B27 Genotyping PCR Kit enables real-time genotyping without the need for prior DNA isolation, allowing for a faster and more cost-effective workflow. Applications HLA-B27 screening in suspected ankylosing spondylitis Molecular support in the diagnosis of spondyloarthritides Testing applications in clinical laboratories Immunogenetics research applications Key features NO DNA-extraction necassary – Direct use of EDTA whole blood possible. Genotyping in real-time – Rapid and efficient workflow without time-consuming DNA isolation. Hot-start formulation – Enables convenient room-temperature reaction setup. >Platform-independent – Compatible with all modern real-time PCR cyclers.. Hydrolysis probe detection (TaqMan principle). Duplex real-time PCR (target + internal control).. Reliable fluorescence-based detection – Compatible with qPCR cyclers featuring FAM and ROX channels.. High specificity based on allele-specific primer/probe design. Two independent HLA-B27 targets (Exon 2 and Exon 3) for enhanced robustness. . Eco-friendly shipping – Stable at room temperature, reducing the need for cold-chain transport. Assay principle The kit is based on allele-specific real-time PCR using hydrolysis probe technology. Detection is performed in a duplex format, allowing simultaneous monitoring of the target signal and an internal control. HLA-B27 detection is based on two independent HLA-B27-specific target regions within the HLA-B gene (Exon 2 and Exon 3). By targeting both regions in parallel, assay robustness is increased and the risk of false-negative results caused by rare sequence variants is reduced. The HLA-B27 signal is detected in the FAM channel. The internal control is detected in the ROX channel and ensures reliable identification of PCR inhibition or insufficient DNA release. Direct PCR from whole blood (no DNA extraction required) The kit is optimized for direct use of EDTA whole blood, enabling a simplified workflow without mandatory DNA extraction. A simple dilution of the blood sample is required. Optional is a short heat pre-treatment step (e.g., 95 °C for 10 minutes) to lyse leukocytes and release DNA. Alternatively, purified genomic DNA can be used as PCR input material. Workflow (overview) 1. Dilute EDTA whole blood 2. Heat pre-treatment (recommended) 3. Set up the qPCR reaction 4. Run real-time PCR 5. Qualitative analysis based on amplification curves Result interpretation Positive: Amplification in the FAM channel (HLA-B27) with a valid internal control signal in the ROX channel.Negative: No FAM signal, but clear amplification of the internal control in the ROX channel. Invalid: No internal control signal (indicating PCR inhibition or incorrect sample preparation). Cycler compatibility A real-time PCR instrument with at least two detection channels is required: FAM and ROX.The assay is compatible with common qPCR platforms supporting these channels. Note This kit is intended for screening applications. In rare cases, sequence variations of specific HLA-B27 subtypes may affect amplification performance. In case of discrepant results or specific populations, confirmatory subtyping may be recommended. Kit contents 2× HLA-B27 Direct Duplex qPCR Mastermix Primer/probe mix (HLA-B27 exon targets + internal control) Manual