NGS

NGS DNA Puri MagBeads is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection and PCR purification for DNA and/or RNA library preparation for Next Generation sequencing. It is compatible with mainstream NGS library prepared kits. NGS DNA Puri MagBeads utilize SPRI (Solid Phase Reverse Immobilization) paramagnetic bead technology to enable DNA purification and size selection during NGS library construction.NGS DNA Puri MagBeads are compatible with most NGS library construction kits (DNA, RNA). The kit allows users to seamlessly replace competitor beads (i.e. AMPure XP Beads) in current bead clean-up and size selection protocols without loss of efficiency.. Why Purification and Quality Control of DNA preps for NGS?The purification step is usually necessary to remove unwanted material that might interfere with sequencing. Some NGS platforms may have tight size requirements, and discarding fragments that are too large or too small can improve sequencing efficiency by far. The optimal library size is given by the sequencing application. It is common to clean up nucleic acids using magnetic beads. Quality control is the last process before sequencing. As Subsequent experiments are time-consuming and expensive, strict quality control steps are required to ensure all samples are appropriate for their applications. Confirming the quality and quantity of DNA increases the reliability of sequencing data! The Genaxxon NGS MagBead DNA purification kit protocol can be adapted to your current liquid handling workstation (e.g., Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.

Genaxxon’s DNA Library Prep Kit for Illumina is your universal solution for preparing high-quality libraries for sequencing on Illumina high-throughput platforms. Designed to convert fragmented DNA input ranging from 1 ng to 1 μg into sequencing-ready libraries, this kit offers unmatched efficiency and reliability for your sequencing projects.WorkflowKey Features:Wide Compatibility: Ideal for various sample types, including low-quality FFPE and cfDNA, as well as conventional DNA samples.High Efficiency: Delivers superior library conversion rates, ensuring maximum yield from your input DNA.Accurate Detection: Enables precise identification of low-frequency mutations, making it perfect for sensitive sequencing applications.Streamlined Workflow: Experience a seamless, four-step workflow that completes in approximately three hours—all within a single reaction tube. This process eliminates the need for intermediate purification and sample transfers, reducing the risk of handling errors and preserving the integrity of your samples. With DNA Repair Enzymes II, the kit effectively repairs a wide range of DNA damages, including nicks, gaps, and oxidized bases, ensuring the highest quality libraries for your sequencing needs. Applications: Whole-genome sequencing Whole-exome or targeted capture sequencing Amplicon sequencing ChIP sequencing Metagenome sequencing Methylation sequencing Optimized for IlluminaThe resulting libraries are fully compatible with standard Illumina® sequencing reagents and protocols, delivering exceptional performance for high-coverage deep sequencing, de novo sequencing, and whole-genome resequencing projects.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

Genaxxon’s DNA Library Prep Kit PLUS for Illumina is an advanced solution designed to enhance your sequencing workflow on Illumina high-throughput platforms. This kit simplifies DNA library preparation by integrating DNA fragmentation, end repair, and dA-tailing into a single step. With adapter ligation, library amplification, and size selection performed directly—without additional purification—the entire process is completed in just 2 hours.WorkflowKey Features:Efficient Workflow: Streamline your process by performing fragmentation, end repair, and dA-tailing with a single enzyme mix, finishing in under 2 hours.Versatile Insert Sizes: Customize your insert sizes by adjusting fragmentation time, while minimizing inversions and chimeras for optimal results.Broad Compatibility: Designed for diverse DNA sources, with strict background bacterial control for accurate pathogen detection.Optimized Performance: The Genaxxon DNA Library Prep Kit PLUS is compatible with DNA from various sources, including challenging samples like FFPE DNA. Enhanced reagent optimization reduces false positives, improving sequencing yield and overall results. This kit is particularly well-suited for constructing PCR-free libraries using 100 ng of high-quality genomic DNA. Applications: Whole-genome sequencing Exon or other targeted sequencing Metagenomic sequencing Optimized for IlluminaGenaxxon’s DNA Library Prep Kit PLUS delivers exceptional performance for a range of sequencing applications, ensuring your libraries are fully compatible with Illumina® sequencing reagents and protocols, making it the ideal choice for efficient, high-quality sequencing preparation.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

Genaxxon NGSClone PCR Mastermix (2x) - High-Fidelity, Versatile, Ready-to-UseOverviewAchieve unparalleled accuracy and efficiency with Genaxxon’s NGSClone PCR Mastermix (2x). This high-fidelity, ready-to-use master mix features advanced hot-start technology and enhanced DNA polymerase for precise amplifications. Ideal for a wide range of templates, including GC-rich samples, it includes all essential components for reliable PCR results with minimal setup.Key FeaturesSuperior Fidelity and Processivity: Powered by a novel DNA polymerase, the NGSClone PCR Mastermix ensures precision with a remarkable 100-fold increase in fidelity compared to standard Taq polymerases. Experience accurate and efficient amplifications, thanks to enhanced extension speed and robust 3’-5’ exonuclease activity.Advanced Hot-Start Function: The antibody-mediated hot-start technology prevents non-specific amplifications by keeping the enzyme inactive at room temperature. This means fewer experimental inconsistencies and higher specificity.Versatile Performance: Whether you’re working with animal, plant, or cDNA templates, the NGSClone PCR Mastermix delivers high amplification efficiency across a diverse range of samples. It is especially effective with GC-rich templates, making it a versatile choice for a variety of PCR applications.Convenience and Ease-of-Use: Simplify your PCR workflow with this ready-to-use master mix. It comes complete with all essential components, requiring only the addition of primers and DNA to get started. Save time and reduce errors with a mix that’s ready to perform right out of the box.Elevate your PCR performance with Genaxxon’s NGSClone PCR Mastermix (2x) and experience the future of high-fidelity amplification.