Next Generation Sequencing - Library Preparation Solutions

NGS DNA Puri MagBeads is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection and PCR purification for DNA and/or RNA library preparation for Next Generation sequencing. It is compatible with mainstream NGS library prepared kits. NGS DNA Puri MagBeads utilize SPRI (Solid Phase Reverse Immobilization) paramagnetic bead technology to enable DNA purification and size selection during NGS library construction.NGS DNA Puri MagBeads are compatible with most NGS library construction kits (DNA, RNA). The kit allows users to seamlessly replace competitor beads (i.e. AMPure XP Beads) in current bead clean-up and size selection protocols without loss of efficiency. Why Purification and Quality Control of DNA preps for NGS?The purification step is usually necessary to remove unwanted material that might interfere with sequencing. Some NGS platforms may have tight size requirements, and discarding fragments that are too large or too small can improve sequencing efficiency by far. The optimal library size is given by the sequencing application. It is common to clean up nucleic acids using magnetic beads. Quality control is the last process before sequencing. As Subsequent experiments are time-consuming and expensive, strict quality control steps are required to ensure all samples are appropriate for their applications. Confirming the quality and quantity of DNA increases the reliability of sequencing data! The Genaxxon NGS MagBead DNA purification kit protocol can be adapted to your current liquid handling workstation (e.g., Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.

Genaxxon’s DNA Library Prep Kit for Illumina is your universal solution for preparing high-quality libraries for sequencing on Illumina high-throughput platforms. Designed to convert fragmented DNA input ranging from 1 ng to 1 μg into sequencing-ready libraries, this kit offers unmatched efficiency and reliability for your sequencing projects.WorkflowKey Features:Wide Compatibility: Ideal for various sample types, including low-quality FFPE and cfDNA, as well as conventional DNA samples.High Efficiency: Delivers superior library conversion rates, ensuring maximum yield from your input DNA.Accurate Detection: Enables precise identification of low-frequency mutations, making it perfect for sensitive sequencing applications.Streamlined Workflow: Experience a seamless, four-step workflow that completes in approximately three hours—all within a single reaction tube. This process eliminates the need for intermediate purification and sample transfers, reducing the risk of handling errors and preserving the integrity of your samples. With DNA Repair Enzymes II, the kit effectively repairs a wide range of DNA damages, including nicks, gaps, and oxidized bases, ensuring the highest quality libraries for your sequencing needs. Applications: Whole-genome sequencing Whole-exome or targeted capture sequencing Amplicon sequencing ChIP sequencing Metagenome sequencing Methylation sequencing Optimized for IlluminaThe resulting libraries are fully compatible with standard Illumina® sequencing reagents and protocols, delivering exceptional performance for high-coverage deep sequencing, de novo sequencing, and whole-genome resequencing projects.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

Genaxxon’s DNA Library Prep Kit PLUS for Illumina is an advanced solution designed to enhance your sequencing workflow on Illumina high-throughput platforms. This kit simplifies DNA library preparation by integrating DNA fragmentation, end repair, and dA-tailing into a single step. With adapter ligation, library amplification, and size selection performed directly—without additional purification—the entire process is completed in just 2 hours.WorkflowKey Features:Efficient Workflow: Streamline your process by performing fragmentation, end repair, and dA-tailing with a single enzyme mix, finishing in under 2 hours.Versatile Insert Sizes: Customize your insert sizes by adjusting fragmentation time, while minimizing inversions and chimeras for optimal results.Broad Compatibility: Designed for diverse DNA sources, with strict background bacterial control for accurate pathogen detection.Optimized Performance: The Genaxxon DNA Library Prep Kit PLUS is compatible with DNA from various sources, including challenging samples like FFPE DNA. Enhanced reagent optimization reduces false positives, improving sequencing yield and overall results. This kit is particularly well-suited for constructing PCR-free libraries using 100 ng of high-quality genomic DNA. Applications: Whole-genome sequencing Exon or other targeted sequencing Metagenomic sequencing Optimized for IlluminaGenaxxon’s DNA Library Prep Kit PLUS delivers exceptional performance for a range of sequencing applications, ensuring your libraries are fully compatible with Illumina® sequencing reagents and protocols, making it the ideal choice for efficient, high-quality sequencing preparation.Compatibility: This kit is fully compatible with Genaxxon’s Unique Dual Index primer pairs. For 24 reactions, use the UDI MidiSet (M4402). For 96 reactions, you can either use 2x UDI Set A (M4403) or 1x UDI Set A (M4403) and 1x UDI Set B (M4404).

T4 DNA Ligase catalyzes the formation of a phosphodiester bonds between 5' phosphate and 3' hydroxyl termini in duplex DNA/RNA. The enzyme can join blunt end and cohesive end termini, repair single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.Cohesive End Ligation:For most cohesive end ligations, a 30 minute incubation at 20°C is sufficient. Incubations at 16°C for 4-16 hours are routinely used for the majority of applications. Ligation of blunt ends and single-base pair overhang fragments requires more enzyme to achieve the same extent of ligation as cohesive end DNA fragments. Ligation may be enhanced by addition of PEG, or by reducing the rATP concentration. - T4 DNA Ligase requires ATP > as a cofactor.- T4 DNA Ligase is strongly inhibited by NaCL or KCl if their concentration exceeds 200mM.- Ligation of blunt-ended and single-base pair overhang fragments requires about 50-times as much enzyme to achieve the same extent of ligations cohesive-end DNA fragments.- Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v) final concentration or hexamine chloride, or by reducing the ATP concentration to 50µM.- To dilute T4 DNA Ligase for subsequent storage at -20°C the usage of a storage buffer containing 50% glycerol is recommended.- To dilute T4 DNA Ligase for immediate use, usage of 1X Reaction Buffer is recommended.Applications • Cloning of restriction enzymes generated DNA fragments • Cloning of PCR products • Connection of double-stranded oligonucleotide linkers or adapters with DNA • site-specific mutagenesis • Amplified fragment length polymorphism (AFLP) • Nicking repair in duplex DNA, RNA or DNA/RNA hybrids • Self-circulation of linear DNA.Definition of T4 Ligase activity2 different definitions of T4 Ligase activities are used!One Weiss unit is defined as the amount of enzyme required to convert 1 nmol of 32P-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37°C, using specified reaction conditions (Weiss, B., et al., (1968) J. Biol. Chem., 243, 4543). Norit is a type of activated carbon.A Cohesive End Unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of lambda DNA (5´ DNA termini concentration of 0.12µM (300µg/mL)) in 20µL of 1X T4 DNA Ligase Buffer in 30 minutes at 16°C. The conversion factor between the two activities is: One Cohesive End Ligation units (CEL units) corresponds to 0.015 Weiss units. One Weiss unit therefore corresponds to 66.67 Cohesive End Ligation units (CEL units).

The Genaxxon NGSClone PCR Mastermix (2x) combines high-fidelity DNA polymerase with antibody-mediated Hot-Start technology for precise, fast, and reliable PCR results. Perfect for challenging templates such as GC-rich DNA, cDNA, or complex plant and animal samples. This ready-to-use master mix contains all essential components – just add primers and template – making it ideal for high-fidelity PCR and NGS library preparation.NGSClone PCR Mastermix (2x) – High-Fidelity & Hot-Start for Accurate PCRMaximum accuracy for demanding PCR applicationsThe Genaxxon NGSClone PCR Mastermix (2x) delivers outstanding performance by combining a novel high-fidelity DNA polymerase with antibody-mediated Hot-Start technology in a convenient ready-to-use PCR master mix. With up to 120× higher fidelity compared to standard Taq polymerases, it ensures highly accurate amplification even for GC-rich templates. Ideal for PCR in NGS workflows, cDNA amplification, and plant or animal DNA analysis.Key Features of the NGSClone PCR Mastermix (2x)Exceptional accuracy – High-fidelity DNA polymerase with strong 3’-5’ exonuclease activity (proofreading) for error-free amplification Fast & efficient PCR – Engineered enzyme structure with enhanced domain for high processivity and faster extension rates Fast amplification – achieves target amplification in just 10–15 seconds per kbHot-Start precision – Antibody blocking keeps the enzyme inactive at room temperature, preventing non-specific amplification and improving specificity Broad applicability – Suitable for GC-rich DNA, cDNA, plant and animal templates, and other difficult targets Easy workflow – Contains polymerase, dNTPs, and optimized buffer; just add primers and DNA Benefits in Your Lab Saves time and reduces pipetting stepsMinimizes cross-contamination Delivers high reproducibility Remains stable after repeated freeze-thaw cycles Applications High-fidelity PCR for error-free cloning PCR of GC-rich templates cDNA amplification NGS library preparation Standard PCR requiring high accuracy Genaxxon’s NGSClone PCR Mastermix (2x) – for fast, precise, and reproducible PCR results with maximum convenience.The Genaxxon NGSClone PCR Mastermix Red (2x) is also available with a red loading dye.

Genaxxon’s 2X Oligo (dT)25 Capture Beads offer a reliable and efficient solution for isolating poly(A) RNA from purified total RNA samples. This capture method is based on the binding between the poly(A) tail at the 3' end of mRNA and the Oligo d(T) sequence on the magnetic microsphere. With a streamlined protocol, the capture process is completed in under 1 hour, making it ideal for fast and accurate RNA preparations. Key Features:High Specificity: Ensures targeted capture of poly(A) RNA from total RNA samples.Fast Protocol: Achieve RNA isolation in under 1 hour.Consistent Results: Every kit batch undergoes rigorous quality control and is verified through library preparation and sequencing, ensuring reliable and reproducible results.Complete Kit: Includes all required reagents, optimized for poly(A) RNA isolation.Applications: RNA purification for downstream applications such as RNA sequencing, RT-PCR, and gene expression analysis. Poly(A) RNA enrichment from total RNA samples for high-throughput sequencing.Seamless Compatibility: Fully compatible with Genaxxon’s FAST RNA-seq Library Prep Kit, offering an integrated workflow for RNA capture and library preparation.

Genaxxon’s rRNA Depletion Module offers a precise and efficient solution for removing ribosomal RNA (rRNA) from total RNA samples, enhancing the quality of RNA for downstream applications such as sequencing and transcriptome analysis. Tailored for prokaryotic RNA, this module effectively targets and depletes rRNA, reducing its interference in RNA sequencing results.Key Features:Specifically designed to deplete rRNA from prokaryotic RNA samples.Efficient Removal: Significantly reduces rRNA levels, enriching non-ribosomal RNA for accurate analysis.Streamlined Process: Simplifies sample preparation, ensuring high-quality RNA for subsequent applications.Rigorous Quality Control: Each batch undergoes thorough testing to ensure consistent performance. Applications: RNA sequencing for prokaryotes Transcriptome analysis of bacterial and other prokaryotic samplesSeamless Compatibility:Fully compatible with Genaxxon’s FAST RNA-seq Library Prep Kit, offering an integrated workflow for RNA capture and library preparation.

Genaxxon’s Unique Dual Index Midi Set is designed for precise and efficient sample preparation in Next Generation Sequencing on Illumina platforms. This set includes 24 unique dual index primer pairs (UDI Primers 1-24), ideal for small to medium-scale projects.Key Features:High Ligation Efficiency: Maximizes library output with minimal adapter dimer formation, ensuring superior performance.Enhanced Detection Accuracy: Effectively prevents data crosstalk, crucial for low-frequency mutation detection and low-abundance pathogen analysis.Truncated Adapters: Includes 15 μM truncated adapters with an 8-nucleotide sample index length for accurate and reliable indexing.Applications: Ideal for DNA or RNA library preparation on Illumina platforms, perfectly compatible with the 24 reactions of: Genaxxon DNA Library Prep Kit for Illumina (M4400) Genaxxon DNA Library Prep Kit PLUS for Illumina (M4401)

Genaxxon’s Unique Dual Index Set A is designed for precise and efficient sample preparation in Next Generation Sequencing on Illumina platforms. This set includes 48 unique dual index primer pairs (UDI Primers 1-48), ideal for medium-scale to large projects.Key Features:High Ligation Efficiency: Maximizes library output with minimal adapter dimer formation, ensuring superior performance.Enhanced Detection Accuracy: Effectively prevents data crosstalk, crucial for low-frequency mutation detection and low-abundance pathogen analysis.Truncated Adapters: Includes 15 μM truncated adapters with an 8-nucleotide sample index length for accurate and reliable indexing.Applications: Ideal for DNA or RNA library preparation on Illumina platforms, perfectly compatible with the 96 reactions of (either 2xUDI Set A or UDI Set A combined with UDI Set B): Genaxxon DNA Library Prep Kit for Illumina (M4400) Genaxxon DNA Library Prep Kit PLUS for Illumina (M4401)

Genaxxon’s Unique Dual Index Set B is designed for precise and efficient sample preparation in Next Generation Sequencing on Illumina platforms. This set includes 48 unique dual index primer pairs (UDI Primers 49-96), ideal for medium-scale to large projects.Key Features:High Ligation Efficiency: Maximizes library output with minimal adapter dimer formation, ensuring superior performance.Enhanced Detection Accuracy: Effectively prevents data crosstalk, crucial for low-frequency mutation detection and low-abundance pathogen analysis.Truncated Adapters: Includes 15 μM truncated adapters with an 8-nucleotide sample index length for accurate and reliable indexing.Applications: Ideal for DNA or RNA library preparation on Illumina platforms, perfectly compatible with the 96 reactions of (either 2xUDI Set A or UDI Set A combined with UDI Set B): Genaxxon DNA Library Prep Kit for Illumina (M4400) Genaxxon DNA Library Prep Kit PLUS for Illumina (M4401)

Genaxxon NGSQuant dsDNA HS Assay Kit for Qubit is a highly efficient, fluorescence-based solution for rapid and sensitive quantification of double-stranded DNA (dsDNA). This kit provides accurate measurements for initial DNA concentrations ranging from 10pg/µL to 100ng/µL, making it ideal for a variety of applications, including next-generation sequencing (NGS) and DNA library quantification. With its easy-to-use format and compatibility with Qubit fluorometers, the kit delivers fast, reliable, and precise dsDNA measurements for laboratories focused on high-quality data. Highlights Fast and accurate quantificationPrecise dsDNA measurement for concentrations ranging from 10pg/µL to 100ng/µLSelective detectionHigh contaminant tolerance - Performs reliably even in the presence of salts, solvents, detergents, and proteins. Complete kit – Includes concentrated assay reagent, dilution buffer, and DNA standards. Optimized for NGS – Perfect for quantifying input DNA for next-generation sequencing and DNA library quantification. Applications The NGSQuant dsDNA HS Assay Kit can be used for:Quantification of input DNA for next-generation sequencing (NGS)DNA library quantificationSuitable for use with Qubit fluorometers for precise dsDNA measurements

Genaxxon NGSQuant 1X dsDNA HS Assay Kit is a rapid, highly sensitive, and accurate solution for the quantitative detection of double-stranded DNA (dsDNA) using fluorescence. This kit is specifically designed for dsDNA, offering excellent linearity in the range of 0.2 ng to 100 ng, with a quantitation range of 10 pg/µL to 100 ng/µL.The user-friendly kit provides a ready-to-use working solution that simplifies the dsDNA quantification process on Qubit® fluorometers or fluorescence microplate readers. It is an ideal choice for large-scale DNA sample quantification in next-generation sequencing (NGS) applications, including input DNA and DNA library quantification. Additionally, the kit demonstrates robust tolerance to common contaminants, such as proteins and salts, ensuring reliable results across various sample types.Highlights Rapid, highly sensitive, and accurate fluorescent detection of double-stranded DNA (dsDNA). Excellent linearity in the range of 0.2 ng to 100 ng. Quantitation range of 10 pg/µL to 100 ng/µL. Ready-to-use working solution for easy operation. Compatible with Qubit® fluorometers and fluorescence microplate readers. Tolerant to common contaminants such as proteins and salts. Applications The NGSQuant 1X dsDNA HS Assay Kit is suitable for: Large-scale DNA sample quantification for next-generation sequencing (NGS). Input DNA quantification for NGS workflows. DNA library quantification for various sequencing applications.

The Genaxxon NGSClone PCR Mastermix Red (2x) combines high-fidelity DNA polymerase with antibody-mediated Hot-Start technology and an integrated red loading dye for precise, fast, and reliable PCR results – with no additional dye needed before gel electrophoresis. PCR products can be loaded directly onto the gel, saving time and reducing handling steps.. Perfect for challenging templates such as GC-rich DNA, cDNA, or complex plant and animal samples. This ready-to-use master mix contains all essential components – just add primers and template – making it ideal for high-fidelity PCR and NGS library preparation.NGSClone PCR Mastermix Red (2x) – High-Fidelity & Hot-Start & Direct Gel Loading for Accurate PCRMaximum accuracy for demanding PCR applicationsThe Genaxxon NGSClone PCR Mastermix Red (2x) delivers outstanding performance by combining a novel high-fidelity DNA polymerase with antibody-mediated Hot-Start technology and an integrated red loading in a convenient ready-to-use PCR master mix. With up to 120× higher fidelity compared to standard Taq polymerases, it ensures highly accurate amplification even for GC-rich templates. Ideal for PCR in NGS workflows, cDNA amplification, and plant or animal DNA analysis. Key Features of the NGSClone PCR Mastermix Red (2x)Exceptional accuracy – High-fidelity DNA polymerase with strong 3’-5’ exonuclease activity (proofreading) for error-free amplification Fast & efficient PCR – Engineered enzyme structure with enhanced domain for high processivity and faster extension rates Fast amplification – achieves target amplification in just 10–15 seconds per kbHot-Start precision – Antibody blocking keeps the enzyme inactive at room temperature, preventing non-specific amplification and improving specificity Integrated red loading dye – PCR products can be loaded directly onto the gel without additional stepsBroad applicability – Suitable for GC-rich DNA, cDNA, plant and animal templates, and other difficult targets Easy workflow – Contains polymerase, dNTPs, and optimized buffer; just add primers and DNA Benefits in Your Lab Saves time and reduces pipetting stepsMinimizes cross-contamination Delivers high reproducibility Direct gel loading without extra reagentsRemains stable after repeated freeze-thaw cycles Applications High-fidelity PCR for error-free cloning PCR of GC-rich templates cDNA amplification NGS library preparation Standard PCR requiring high accuracy Genaxxon’s NGSClone PCR Mastermix Red (2x) – for fast, precise, and reproducible PCR results with maximum convenience.The Genaxxon NGSClone PCR Mastermix (2x) is also available without red loading dye.