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Bioactive Proteins
Endotheliales Rindermitogen (ECGS) - Calmodulin - Natürliches Mauslaminin - rHu cellular Fibronectin - rHu Decorin - rHu EpCAM - rHu HER2-ECD - rHu Thrombospondin - rHu Vitronectin
Chymotrypsinogen A - Chymotrypsin precursor
From
€551.67*
Chymotrypsinogen is the practically inactive "precursor" (proenzyme, or zymogen) of chymotrypsin. In order to be activated, chymotrypsinogen has to be cleaved by trypsin between the amino acids arginine and isoleucine (R15 and I16) , which leads to structural changes and the formation of the substrate binding site (Sears 2010). Chymotrypsin is a trypsin-like serine protease.
Chymotrypsin differs from trypsin in the selectivity of the cleavage site of proteins. While trypsin cleaves proteins between the amino acids Arg and Lys, Chymotrypsin prefers large hydrophobic amino acids as cleavage site.
Unit definition: The amount of activated zymogen that causes a decrease in absorbance at 237 nm of 0.0075 per minute at 25°C resulting from the hydrolysis of ATEE (NF/USP unit).
Heparin sodium salt from porcine intestinal mucosa
€519.05*
From
€136.93*
Heparin is a polysaccharide classified as mucopolysaccharide or glycosaminoglycan. In the organism, it is especially formed and stored in mast cells of various mammalian tissues such as liver, lung and mucous membrane. Heparin is mainly isolated from bovine lung, or pig intestine (intestinal mucosa).
Heparin is mainly responsible for delayed blood clotting. It enhances the antithrombin-mediated inactivation of proteases in the clotting pathway. Therefore, it is also often used as an anticoagulant in blood sampling. Thus, it binds to antithrombin III, a naturally occurring plasma protease inhibitor, thereby increasing the rate at which antithrombin III (AT-III) inhibits the coagulation of coagulating proteases, such as factor Xa or thrombin.
Heparin from Genaxxon bioscience is derived from porcine intestinal mucosa (as sodium salt, specific activity >150 units/mg). It is lyophilized and stabilizer-free. Common concentrations are 5000 units/mL. Thus, about 34 mg should be reconstituted with 5mL ultrapure water and then sterile filtered.
In solution, heparin is stable at +2°C to +8°C for up to 2 years, when previously filtered through a 0.2mm sterile filter. CAUTION: Microorganisms rapidly degrade heparin, as they use the polysaccharide side chains as a source of carbon for their own growth.
Variants from €136.93*
Albumin from hen egg white - Ovalbumin
€226.29*
Albumin from hen egg white (ovalbumin) is a phosphorylated glycoprotein that contains 385 amino acid residues and has a molecular weight of 42.7 kDa. Chicken egg white is the protein component with the highest amount of egg white.
It is used in protein research as a marker (approx. 45 kDa) in protein electrophoresis. In addition, it is used for structure elucidation and also serves as a comparison standard.
Bovine albumin crystallized, free of fatty acid, free of globulin
€472.42*
From
€119.76*
Albumin - crystallised is a Fraction V Albumin recrystallised 3 times at temperatures below 0°C. This procedure ensures a very native protein, that is free of carbohydrates, globulines and fatty-acids.
Variants from €119.76*
Bovine albumin Fraction V (pH 7.0)
From
€77.58*
Standard grade, lyophilised Albumin. Bovine serum albumin (BSA) is added as a stabilizing component for proteins/enzymes to several enzyme reaction and storage buffers. The concentration usually ranges from 0.01% (0.1mg/mL) to 3% (30mg/mL). BSA is added to the 10X concentrated buffers of DNA-modifying enzymes or restriction enzymes in a concentration of 0.5mg/mL. Alternatively, BSA can be substituted by gelatin for such purposes at the same concentration. Besides, albumin is applied as a blocking agent for blocking unbound surfaces of blotting membranes in immunoblots (3%) or ELISAs (3% in PBS) or for the dilution of antisera and antibody-stock solutions, respectively. In ELISAs, BSA is frequently replaced by non-fat dried milk. This fraction of albumin has been manufactured by a combination of the heat-shock method and alcohol precipitation. Albumin is stable as powder (3 years) or in solution (biological buffers like PBS, one year at +4°C to -20°C). Stock solutions are prepared in concentrations up to 20%. If crystals are formed during storage of the solutions, they can be redissolved by warming up to 37°C and mixing. Usually, sodium azide is added at a final concentration of 2 mM (or 0.02 - 0.2%) to prevent microbial contaminations.
Bovine albumin for EIA and RIA
From
€65.29*
IgG-free bovine serum albumin. Especially recommended as a blocking and stabilising reagent in all antibody-mediated detection systems. In addition to being IgG-free, this albumin also shows a very low fatty acid content of less than 0.01%.
Feature:Prepared from bovine plasma or serum obtained from cattle less than 30 months old which have received ante and post mortem inspectors by USFDA inspected facilities.
Bovine Serum Albumin, very low endotoxin, no IgG
€273.88*
From
€91.29*
Heat treated (equivalent to 10 hours at 60°C) Bovine Serum Albumin with a very low endotoxin value and no detectable IgG. In addition this albumin is free of mycoplasma and bovine viruses. The isolation and purification process prevent the product from impurities like capryllic acid or other stabilisers that could be cytotoxic to some cell lines. Tested for use in tissue culture.
Variants from €91.29*
alpha-Chymotrypsin (EC 3.4.21.1) from Porcine
From
€99.03*
alpha-Chymotrypsin is a serine peptidase that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Leu) on the carboxyl end of the bond. Ca2+ activates and stabilizes the enzyme. The enzyme has 241 amino acid residues in total contained in three polypeptide chains (A chain: 13 amino acids; B chain: 131 amino acids, and C chain: 97 amino acids) linked by disulfide bridges.
alpha-Chymotrypsin is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF) >, N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK) >, chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10mM Cu2+ and Hg2+.