Rapid DNA Extraction

The non-toxic Plant DNA Q-Extraction Solution provides fast and easy extraction of nucleic acids from various plant material (e.g. ivy or stinging nettle). The PCR-ready nucleic acid is extracted, using one tube and one reaction, in as little as 8 minutes. The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is then ready for PCR without further handling such as vortex, centrifugation or dilutions. The obtained DNA extract is stable at -20°C for up to one week and at -80°C for long term storage. For optimal genotyping results we recommend to use our Plant DNA Q-Extraction Solution together with our InhibiCore Rapid Plant PCR MasterMix (5X). We also offer them as a complete InhibiCore Rapid Plant DNA Q-Extraction PCR Kit for fast direct PCR from plant material. Features:- One-reagent set-up- Minimal handling- Rapid 8-minute protocol- PCR-ready DNA- no sample / yield loss- DNA extracts from plants- Non-toxic reagents- Scalable set-up- Automation-friendly Extraction ProtocolPreparation of DNA extraction should be performed in a separate area from that used for setting up the PCR reaction. 1. Thaw Plant DNA Q-Extraction Solution. For the first time use, mix the Plant DNA Q-Extraction solution well and aliquote it into smaller volumes. (Plant DNA Q-Extraction Solution has a cloudy appearance).2. Add your sample to a tube containing 100µL Plant DNA Q-Extraction Solution. Recommended sample sizes are shown in the table of the manual.3. Vortex the tube containing the sample and the DNA extraction solution for 15 sec. Make sure that the sample is completely covered by the Plant DNA Q-Extraction Solution.4. Transfer the tube to a heat block or a thermal cycler and incubate for 1. 65 °C for 6 min2. 98 °C for 2 min3. 4 °C (or cool down on ice) The DNA extract is now ready for PCR. Please note: Plant DNA lysate should be diluted 5- to 10-fold prior to PCR. We recommend performing a serial dilution. Applications examples:1. Sensitive qPCR analysis of chlorophyll gene targets from wheat lysatesReliable and sensitive detection of chlorophyll gene targets from plant lysates was demonstrated using qPCR with the InhibiCore Rapid Plant PCR MasterMix (5X) (M3250) and fluorescent dye chemistry. A serial dilution of wheat lysate (10% to 0.001%) was used to evaluate sensitivity and inhibitor tolerance.Successful amplification was achieved across all dilutions, confirming strong robustness against plant-derived inhibitors even at high lysate concentrations. A single, specific melt curve peak confirmed assay specificity with no non-specific amplification. Samples were prepared using the rapid 8-minute Plant DNA Q-Extraction Solution protocol, enabling fast DNA preparation without conventional purification steps. Figure 1: qPCR data by wheat lysate qPCR with InhibiCore Rapid Plant PCR MasterMix (5X).The figure shows qPCR amplification curves and melting curve analysis of a wheat lysate dilution series (10% to 0.001%) and a no-template control (NTC) using InhibiCore Rapid Plant PCR MasterMix (5X) and 1xGreenDye. Clear, concentration-dependent amplification is observed in the FAM channel, with reduced signal intensity at 10% lysate due to matrix effects. Melting curve analysis reveals a single specific peak for all positive samples, confirming specific target amplification without non-specific products. 2. Benchmark of Direct PCR performance from 10% tomato lysateThe performance of the InhibiCore Rapid Plant PCR MasterMix (5X) (M3250) was evaluated against three competitor master mixes for qPCR from 10% tomato lysate. Samples were prepared using the Plant DNA Q-Extraction Solution and analyzed by fluorescent dye detection in the FAM channel.The InhibiCore Master Mix showed successful amplification with the earliest Ct values, demonstrating superior efficiency and the highest inhibitor tolerance compared to all tested competitors. Two competitor systems also produced amplification, while two showed strong inhibition with no detectable signals. Specific amplification was confirmed by a single, distinct melt curve peak with no evidence of non-specific products or primer-dimer formation.Figure 2: Plant direct PCR from plant samples Benchmark - inhibitor effect from Tomato lysateDirect PCR was performed using a cycling protocol of 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 sec and 60 °C for 30 sec, with subsequent melt curve analysis. InhibiCore Rapid Plant PCR MasterMix (5X) and Competitor N showed successful amplification, while Competitor T and Competitor Q were strongly inhibited and did not generate reliable amplification curves. InhibiCore Rapid Plant PCR MasterMix (5X) exhibited the earliest Ct value among all tested systems, indicating the highest tolerance to plant-derived PCR inhibitors and superior performance in crude tomato lysates. 3.  Easy sample handlingPlant DNA Q-Extraction Solution was used to extract genomic DNA from different plant tissues. For DNA extraction, 10mg tissue (leave) was added to 100µL of Plant DNA Q-Extraction Solution.Figure 3: DNA extracts of stinging needle and ivy. PCR tubes in duplicate with 100µL Plant DNA Q Extract Solution + 10mg leave material from either stinging neetles or ivy. A. stinging neetle and B. Ivy before extraction with Plant DNA Q Extraction Solution. C. stinging neetle and D. Ivy after extraction with Plant DNA Q Extraction Solution.Figure 4. The extracted DNA from ivy was amplified and two primer sets targeting: A. Genomic plant DNA (ITS 335 bp) or B. Chloroplast DNA (trnL 380 bp). The positive controls are 1 ng/reaction of DNA purified from stinging nettle using DNeasy Plant Mini Kit (Qiagen). Also included are duplicates of samples extracted in buffer without lysing agents, but using the Plant DNA Q-Extraction protocol (No treatment). All DNA extracts are amplified in duplicates.  More information? Watch our explainer video:

Direct PCR from Plant Samples – Fast, Robust and Inhibitor-ResistantThe InhibiCore Rapid Plant DNA Q-Extraction PCR Kit is a streamlined, high-performance solution for rapid DNA analysis from plant material. It combines the efficient Plant DNA Q-Extraction Solution (M3230) with the highly robust InhibiCore Rapid Plant PCR MasterMix (5X) (M3250), enabling a complete workflow from sample preparation to reliable qPCR analysis.Plant tissues often contain strong PCR inhibitors such as polyphenols, polysaccharides, and other secondary metabolites. This kit is specifically designed to overcome these challenges, delivering reproducible and highly sensitive qPCR results even from difficult plant matrices.The Plant DNA Q-Extraction Solution enables fast and efficient DNA release without the need for conventional, time-consuming extraction procedures. Combined with the inhibitor-resistant PCR master mix, it creates a seamless Direct-PCR workflow that significantly reduces hands-on time and simplifies the overall process.The included InhibiCore Rapid Plant PCR MasterMix (5X) is based on a modified HotStart Taq DNA polymerase, ensuring high specificity, sensitivity, and robustness. Its exceptional inhibitor tolerance makes it ideally suited for demanding applications in plant research, agriculture, and quality control. The kit is also lyo-ready and includes dUTP for optional carry-over contamination control when used with UDG (Uracil-DNA Glycosylase). The HotStart polymerase enables convenient room-temperature setup without cooling or activation steps.Key BenefitsComplete solution for fast direct PCR from plant materialSeamless workflow: DNA extraction + qPCR in one systemHigh inhibitor tolerance for robust amplification from plant samplesFast and simple sample preparation without classical DNA isolationHigh sensitivity and specificity for reliable resultsTypical Applications Plant genotyping GMO and transgene detection Agricultural and plant research Plant pathogen detection Seed and quality control High-throughput direct PCR from plant material Applications examples:1. Sensitive qPCR analysis of chlorophyll gene targets from wheat lysatesReliable and sensitive detection of chlorophyll gene targets from plant lysates was demonstrated using qPCR with the InhibiCore Rapid Plant PCR MasterMix (5X) and fluorescent dye chemistry. A serial dilution of wheat lysate (10% to 0.001%), prepared with Plant DNA Q-Extraction Solution (M3230) was used to evaluate sensitivity and inhibitor tolerance.Successful amplification was achieved across all dilutions, confirming strong robustness against plant-derived inhibitors even at high lysate concentrations. A single, specific melt curve peak confirmed assay specificity with no non-specific amplification. Samples were prepared using the rapid 8-minute Plant DNA Q-Extraction Solution protocol, enabling fast DNA preparation without conventional purification steps. Figure 1: qPCR data by wheat lysate qPCR with InhibiCore Rapid Plant PCR MasterMix (5X).The figure shows qPCR amplification curves and melting curve analysis of a wheat lysate dilution series (10% to 0.001%) and a no-template control (NTC) using InhibiCore Rapid Plant PCR MasterMix (5X) and 1xGreenDye. Clear, concentration-dependent amplification is observed in the FAM channel, with reduced signal intensity at 10% lysate due to matrix effects. Melting curve analysis reveals a single specific peak for all positive samples, confirming specific target amplification without non-specific products. 2. Benchmark of Direct PCR performance from 10% tomato lysateThe performance of the InhibiCore Rapid Plant PCR MasterMix (5X) was evaluated against three competitor master mixes for qPCR from 10% tomato lysate. Samples were prepared using the Plant DNA Q-Extraction Solution and analyzed by fluorescent dye detection in the FAM channel.The InhibiCore Master Mix showed successful amplification with the earliest Ct values, demonstrating superior efficiency and the highest inhibitor tolerance compared to all tested competitors. Two competitor systems also produced amplification, while two showed strong inhibition with no detectable signals. Specific amplification was confirmed by a single, distinct melt curve peak with no evidence of non-specific products or primer-dimer formation.Figure 2: Plant direct PCR from plant samples Benchmark - inhibitor effect from Tomato lysateDirect PCR was performed using a cycling protocol of 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 sec and 60 °C for 30 sec, with subsequent melt curve analysis. InhibiCore Rapid Plant PCR MasterMix (5X) and Competitor N showed successful amplification, while Competitor T and Competitor Q were strongly inhibited and did not generate reliable amplification curves. InhibiCore Rapid Plant PCR MasterMix (5X) exhibited the earliest Ct value among all tested systems, indicating the highest tolerance to plant-derived PCR inhibitors and superior performance in crude tomato lysates.

The non-toxic DNA Q-Extraction Solution provides fast and easy extraction of nucleic acids from various mammalian tissues (e.g. mouse tails, ear snips, liver, kidney, lung), saliva and bacteria. The PCR-ready nucleic acid is extracted, using one tube and one reaction, in as little as 8 minutes. The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is then ready for PCR without further handling such as vortex, centrifugation or dilutions. The obtained DNA extract is stable at -20°C for up to one week and at -80°C for long term storage.Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types. For optimal genotyping results we recommend to use our DNA Q-Extraction Solution together with our Red Mastermix Master Mix (2X) for PCR (M3029) or our SuperHot Mastermix Blue (2X) (M3007b). E.g. use 2µL to 5µL of the Q-Extract solution for a 25µL PCR reaction. For plant tissue we recommend using our specific Plant DNA Q-Extraction Solution (M3230). Features:- One-reagent set-up- Minimal handling- Rapid 8-minute protocol- PCR-ready DNA- no sample / yield loss- DNA extracts from mammalian tissues and bacteria- Non-toxic reagents- Scalable set-up- Automation-friendly Extraction ProtocolPreparation of DNA extraction should be performed in a separate area from that used for setting up the PCR reaction. 1. Thaw DNA Q-Extraction Solution. For the first time use, aliquot the DNA Q-Extraction solution into smaller volumes. (DNA Q-Extraction Solution has a cloudy appearance).2. Add your sample to a tube containing 100µL DNA Q-Extraction Solution. Recommended sample sizes are shown in Table 1 of the data sheet.3. Vortex the tube containing the sample and the DNA extraction solution for 15 sec. Make sure that the sample is completely covered by the DNA Q-Extraction Solution.4. Transfer the tube to a heat block or a thermal cycler and incubate for 1. 65 °C for 6 min2. 98 °C for 2 min3. 4 °C (or cool down on ice) The DNA extract is now ready for PCR. Anwendungsbeispiele:Efficient Amplification of DNA extracts from various mammalian tissue.DNA Q-Extraction Solution was used together with our RedMastermix (M3029 >) to extract and amplify genomic DNA from various mammalian tissues. For DNA extraction, 0.5-10 mg tissue or 20µL salvia was added to 100µL of DNA Q-Extraction Solution.M: DNA marker Iqon Low DNA Ladder. Lane 1-5: Different mouse tissues as depicted, GAPDH (266 bp). Lane 6: Chicken muscle tissue, HRPT1 (245 bp) and Lane 7: Human saliva, DMD17 (415 bp). DNA Q-Exctract Solution works over a wide range of sample sizesIn order to investigate the flexibility of DNA Q-Extraction Solution protocol, varying amounts of chicken muscle tissues were added to 100µL of DNA Q-Extraction Solution. The extraction was conducted as specified in the datasheet for DNA Q-Extraction Solution. The extracted DNA was subjected to PCR using the Genaxxon RedMastermix (M3029 >). The results show that the DNA Q-Extraction protocol provides the user with a high degree of flexibility over a wide range of applied sample amount.M: DNA marker Iqon Low DNA Ladder. Lanes 1-28: Varying amounts (mg) of chicken muscle tissue, HRPT1 (245 bp). Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527

Fast DNA Extraction & Hot-Start PCR KitThe DNA Q-Extraction Hot-Start PCR Kit combines our rapid DNA Q-Extraction Solution with the high-performance SuperHot Mastermix (2X) Blue. This all-in-one kit enables fast DNA extraction and reliable Hot-Start PCR – with PCR-ready DNA in just 8 minutes, without any additional purification steps. Ideal for genotyping and the amplification of DNA from a wide range of sources including mammalian tissues, bacterial cells, saliva, and oral swabs.Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.Key FeaturesCombined DNA extraction & hot-start PCRFast 8-minute protocolNo centrifugation neededPCR-ready DNA from cells and tissuesIncludes loading dye for direct gel loading and pipetting controlApplicationThe simple one-step lysis is performed in two heating steps (65 °C / 98 °C) using a thermocycler or heating block. The resulting lysate contains PCR-ready DNA, suitable for standard and real-time PCR, without vortexing, or centrifugation.The included SuperHot Mastermix (2X) Blue contains a hot-start Taq polymerase for improved specificity and sensitivity. The integrated blue loading dye allows direct gel loading and visual control of pipetting.Kit ComponentsDNA Q-Extraction SolutionSuperHot Mastermix (2X) BlueStorageExtracted DNA is stable at -20 °C for up to one week, and for long-term storage at -80 °C.Also available as a non-hot-start version with our reliable Red Mastermix (2X) – perfect for robust results in routine PCR.Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527

Fast DNA Extraction & PCR in One KitThe DNA Q-Extraction PCR Kit Red combines the powerful DNA Q-Extraction Solution with our reliable Red Mastermix (2X) – enabling rapid and easy DNA extraction followed by PCR amplification in a single streamlined workflow. Perfect for genotyping, this kit delivers PCR-ready DNA in just 8 minutes from a variety of sample types such as mammalian cells, bacteria, saliva, buccal swabs, or tissue samples.Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.Your Benefits at a GlanceFast 8-minute protocolNo vortexing, centrifugation or dilutionPCR-ready DNA directly from lysateSimple protocol: lysis and PCR with minimal handlingNon-toxic reagents – safe and convenientScalable and automation-friendlyDirect gel loading thanks to built-in loading dyeApplicationThe lysis is performed in two simple heating steps (65 °C for 6 min, 98 °C for 2 min) using a thermocycler or heating block. The resulting DNA is immediately ready for PCR without any further purification – ideal for genotyping applications. The included Red Mastermix (2x) ensures reliable amplification results and enables direct loading onto agarose gels with visual pipetting control.Kit ComponentsDNA Q-Extraction SolutionRed Mastermix (2X)Storage & StabilityExtracted DNA is stable at -20 °C for up to one week. For long-term storage, we recommend -80 °C.Also available as a hot-start version with our SuperHot Mastermix (2X) Blue – ideal for increased specificity with complex templates.Application Notes:application note genotyping with m3231_dna_q_extraction_solution_en_vs250527