Advantages at a glance
- for identification of lac+ bacterial colonies.
- An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal)
- producing a darker blue color compared to X-Gal
Product number:
M3225.0100
Our benefits
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
For precise information regarding the delivery date, please contact Genaxxon.
Product information "Bluo-Gal / 5-Bromo-3-indolyl-beta-D-galactopyranoside"
5-Bromo-3-indolyl-β-D-galactopyranosid (Bluo-Gal) ist used as a substrat of β-Galactosidase. It is a chromogenic substrate suitable for identification of lacZ+ bacterial colonies. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Principle
When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.
Specifications:
Assay: min. 98.0% (HPLC)
Appearance: white to white-off powder
C14H16BrNO6
MW = 374.18 g/mol
Soluble in: DMF
Application:
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color. Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.Source:
syntheticClassification:
CAS no.:
97753-82-7
eclass no.:
32160490
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Documents:
Safety Data SheetCertificate
Category List
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed
Source: NCBI PubMed