Advantages at a glance
- Broad band antibiotic for usage in cell culture applications
- 50mg/mL solution
- Sterile filtered
Product number:
M3123.0050
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Product information "Gentamycin sulfate solution (50mg/mL)"
Gentamycin is an almost ideal antibiotic for cell culture as it has a broad spectrum of antibacterial and antimycoplasmic activity. Gentamycin has biological and biochemical properties that make it superior to Penicillin and Streptomycin (PS). In cell culture, gentamycin is normally used at a concentration of 25 to 50μg/mL in the growth medium. Higher concentrations, e.g. 200μg/mL, are often used in viral studies when samples are known to be infected.
As a broad-spectrum antibiotic, it can be used more widely and effectively than Penicillin/Streptomycin. Gentamicin is not only active against many Gram-positive and Gram-negative organisms, but also against Proteus and Staphylococcus strains, which can sometimes be resistant to a PS mixture. In addition, gentamicin is also active against Pseudomonas strains.
Influence of the pH value
Gentamicin shows good pH stability and has been shown to be stable in tissue culture media for 15 days, in a pH range of 2 to 10 and +37 °C. These are significant advantages over streptomycin, which is rapidly destroyed by alkaline conditions, and penicillin, which is unstable at both acidic and alkaline pH.
Temperature stability
Gentamicin is stable at all temperatures and can even withstand autoclaving. When using autoclavable powder media, gentamicin can therefore be added before sterilization. In contrast, autoclaving leads to almost complete inactivation of penicillin and reduces the activity of streptomycin by 50%.
Serum compatibility
Compared to pencillin, the activity of gentamycin is not affected by the presence of serum. Penicillin, on the other hand, immediately loses 30% of its activity when serum is added to the tissue culture medium.
Cell tolerance and other aspects
- Cells tolerate more than 20 times the normal concentration.
- The use of gentamicin has no effect on viral yield or plaque formation.
- Gentamicin has no effect on interferon yield or assays.
- The use of gentamicin has no effect on viral yield or plaque formation.
- Gentamicin has no effect on interferon yield or assays.
Further use as an additive in mycological media to prevent bacterial growth.
Gentamycin is ideal for use in cell and tissue cultures or the transport of cell cultures and clinical samples containing viruses.
Specifications:
50mg/mL solution
sterile filtered
50mg/mL solution
sterile filtered
broad band antibiotic for usage in cell culture applications
Application:
cell culture; recommended working concentrations: 0.5 up to 50µg/mLSource:
syntheticClassification:
eclass no.:
32199090
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