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Product information "4,5-Diaminofluorescein - DAF-2"
DAF-2 (4,5-Diaminofluorescein) shows higher photostability than the classical fluorescein derivative DAF. Applications: DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxideproducing neurons in brain slices, in mitochondria and in living plant cells.
In the cell, the product reacts rapidly with NO in the presence of O2 to form the highly fluorescent compound triazolofluorescein (DAF-2T (Cat-No. S5442 >)).
References: (1) M. Kirsch et al.; J. Pineal Res. 40(1), 10 (2006), (2) V.M. Dirsch et al.; Biol. Proc. Online 5(1), 136 (2003), (3) N. Nagata et al.; J. Biochem. 125(4), 658 (1999), (4) H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998), (5) H. Kojima et al.; Anal. Chem. 70, 2446 (1998)
Specifications:
Assay: min. 95% (HPLC)
Appearance: Light yellow powder
Identity: determined by NMR
C20H14N2O5
MW = 362.34 g/mol
Fluorescence: λex 505 nm; λem 518 nm in 0.1M Tris pH8.0
Solubility: soluble in DMSO
Application:
in-vivo detection of NO and the determination of nitric oxide synthase.Source:
syntheticSafety Information:
Classification:
Documents:
Safety Data SheetCertificate
Category List
Source: NCBI PubMed
Accessory Items
4,5-Diaminofluorescein triazole (DAF-2T) can be used as a reference material for DAF-2 (S5439 >).References: (1) M. Feelisch et al;, Free Radical Biol. Med. 38(3), 356 (2005); (2) T. Nagano et al.; J. Biol. Chem. 277(1), 47 (2002); (3) T. Nagano et al.; FEBS Letters 427(2), 263 (1998); (4) H. Kojima et al.; Chem. Pharm. Bull. 46(2), 373 (1998)
4,5-Diamino-N,N,N',N'-tetraethyl-rhodamine. Sensitive NO probe, LOD of 10 nM, shows higher photostability than the classical fluorescein derivative DAF.