InhibiCore Rapid Plant PCR MasterMix (5X)

Fast and Reliable qPCR from Plant Samples

The InhibiCore Rapid Plant PCR MasterMix (5X) is specifically designed for fast, reliable, and highly sensitive qPCR from plant material.

Plant samples often contain PCR inhibitors such as polyphenols, polysaccharides, and other secondary metabolites that can negatively affect amplification efficiency. Engineered for exceptional inhibitor tolerance, the InhibiCore Rapid Plant PCR MasterMix delivers robust and reproducible amplification even from challenging plant-derived samples.

The included modified HotStart Taq DNA Polymerase provides outstanding specificity and sensitivity while minimizing non-specific amplification. This ensures reliable and accurate results, even in demanding applications. The master mix contains an optimized PCR buffer, dNTPs, and MgCl₂, enabling convenient and streamlined reaction setup. Since the HotStart polymerase remains inactive at room temperature, no cold setup is required, simplifying workflow and reducing the risk of non-specific amplification.

The master mix is also lyo-ready, making it an excellent choice for the development of stable, room-temperature assay formats. In addition, the incorporation of dUTP enables effective carry-over contamination control when used together with UDG (Uracil-DNA Glycosylase).

For rapid direct PCR workflows, we recomment the InhibiCore Rapid Plant DNA Q-Extraction PCR Kit (M3252), providing a fast and efficient sample preparation method that integrates seamlessly with downstream qPCR applications.

Key Benefits

• Specifically optimized for plant samples and inhibitor-rich plant matrices
• Exceptional inhibitor tolerance for reliable amplification
• High sensitivity and specificity for accurate target detection
• Convenient room-temperature setup with HotStart technology
• Lyo-ready and dUTP-enabled contamination control capability
  

Typical Applications

• Plant genotyping
• Plant pathogen detection
• Agricultural and plant research
• GMO testing
• Seed quality control
• Direct PCR from plant material

Applications examples:

1. Sensitive qPCR analysis of chlorophyll gene targets from wheat lysates

Reliable and sensitive detection of chlorophyll gene targets from plant lysates was demonstrated using qPCR with the InhibiCore Rapid Plant PCR MasterMix (5X) and fluorescent dye chemistry.
A serial dilution of wheat lysate (10% to 0.001%), prepared with Plant DNA Q-Extraction Solution (M3230) was used to evaluate sensitivity and inhibitor tolerance.

Successful amplification was achieved across all dilutions, confirming strong robustness against plant-derived inhibitors even at high lysate concentrations. A single, specific melt curve peak confirmed assay specificity with no non-specific amplification. Samples were prepared using the rapid 8-minute Plant DNA Q-Extraction Solution protocol, enabling fast DNA preparation without conventional purification steps.

Figure 1: qPCR data by wheat lysate qPCR with InhibiCore Rapid Plant PCR MasterMix (5X).
The figure shows qPCR amplification curves and melting curve analysis of a wheat lysate dilution series (10% to 0.001%) and a no-template control (NTC) using InhibiCore Rapid Plant PCR MasterMix (5X) and 1xGreenDye. Clear, concentration-dependent amplification is observed in the FAM channel, with reduced signal intensity at 10% lysate due to matrix effects. Melting curve analysis reveals a single specific peak for all positive samples, confirming specific target amplification without non-specific products.

2. Benchmark of Direct PCR performance from 10% tomato lysate

Figure 2: Plant direct PCR from plant samples Benchmark - inhibitor effect from Tomato lysate
Direct PCR was performed using a cycling protocol of 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 sec and 60 °C for 30 sec, with subsequent melt curve analysis. InhibiCore Rapid Plant PCR MasterMix (5X) and Competitor N showed successful amplification, while Competitor T and Competitor Q were strongly inhibited and did not generate reliable amplification curves. InhibiCore Rapid Plant PCR MasterMix (5X) exhibited the earliest Ct value among all tested systems, indicating the highest tolerance to plant-derived PCR inhibitors and superior performance in crude tomato lysates.