Ni-NTA Agarose for His-tagged proteins

1,3-Diaza-2,4-cyclopentadiene, Glyoxalin

Hydrated gel filtration columns for protein purification and desalting. Processes sample volumes of 150 to 300µL. CentriPure 2 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a sample volume of up to 200µL can be purified with an elution volume of 200 to 350µL.

This tablets are designed for the purification of histidine tagged and non-histidine tagged recombinant and native proteins using IMAC chromatography. The phosphate buffer may be used for other types of affinity chromatography methods. Immobilized metal ion affinity chromatography (IMAC) is a widely used separation method for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also some untagged recombinant or native proteins. Genaxxon's IMAC phosphate and elution buffer tablets have been developed exclusively for use in this method. IMAC buffer tablets are supplied in bottles and in blister packs. One tablet of phosphate buffer dissolved in deionized water yields a 300mM sodium chloride, 50mM sodium phosphate buffer with pH8.0 at 22°C. One tablet of elution buffer yields a 300mM sodium chloride, 300mM imidazole, 50mM sodium phosphate buffer with pH8.0 at 22°C. Features: Dissolve and go, Exactly pre-weight tablets, just mix elution buffer with phosphate buffer to achieve desired imidazole concentration, ultrapure, low UV280nm absorbance imidazole.

CentriPure P100 columns are pre-hydrated gel filtration columns for protein purification and desalting. CentriPure P100 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a sample volume of up to 10mL can be purified with an elution volume of 12mL to 15mL.

Nickel-loaded agaroses are the most frequently used products for the efficient purification of 6×histidine-tagged recombinant fusion proteins. Because Ni2+ ions show high affinity for 6×histidine-tagged fusion proteins, extremely high yields result from purification of these fusion proteins from the lysates. However, if purification takes place from lysates containing foreign protein chains with repeated histidine elements, selectivity with respect to 6×histidine-tagged fusion protein decreases as non-tagged histidine-containing proteins increasingly also bind to the Ni-IDA agarose. In this case the use of Genaxxon Co-IDA Agarose is recommend (cat#: S5370), which features far higher selectivity with respect to 6×histidine-tagged fusion protein. Genaxxon Ni-IDA Agarose has high chemically and pH stability and enables purification directly from raw lysates. Genaxxon Ni-IDA Agarose can be regenerated several times with nickel sulfate solution for re-use. Features of Ni-IDA-Agarose - a highly efficient and favorable way for the separation of 6-His tagged fusion proteins. Highlights: - ideal for grafity-flow and batch procedures - no expensive purification system necessary - consistent performance - for native and denaturing conditions. The Genaxxon Ni-IDA Agarose consists of cross-linked Agarose, covalently bound to Iminodiacetic acid loaded with Ni2+-ions. Binding capacity: up to 50mg 6xHis tagged fusion protein per mL Agarose. Application: Affinity chromatography of 6-His tagged fusion proteins in batch or grafity flow procedure. Ni-IDA Agarose is supplied as a 50% buffered suspension with 20% Ethanol.