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GelRed(TM) is an ultra sensitive, extremely stable fluorescent dye designed to replace the toxic and possibly mutagenic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed(TM) is far more sensitive than EtBr without requiring a destaining step. GelRed(TM) is far less toxic and mutagenic compared to EtBr while both show virtually the same UV-spectra, so you can directly replace EtBr with GelRed(TM) without changing your existing imaging system.
GelRed(TM) can be used to stain dsDNA, ssDNA or RNA in agarose gel via either precast or post gel staining. GelRed(TM) can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. GelRed(TM) is also compatible with downstream DNA manipulations such as digestion with a restriction enzyme, Southern blotting techniques and clonings. A series of safety tests has confirmed that GelRed(TM) is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed(TM) can be safely disposed in regular trash, providing convenience and reducing cost in waste disposal.
This fluorescent dye is supplied as a 10,000X solution in water. For customers who look for large pack size, we offer a cost-saving bulk pack size of 2mL, 5mL or 10mL (M3199.1010).
- Safer than EtBr: Shown by Ames test and other tests to be nonmutagenic and noncytotoxic. - Easy disposal: Passed environmental safety tests for direct disposal down the drain or in regular trash. - Ultra-sensitive: Much more sensitive than EtBr and ethidium bromide alternative SYBR® Safe. - Extremely stable: Stable at room temperature for long-term storage. - Compatible with standard UV transilluminator or visible light gel reader: GelRed™ replaces EtBr with no optical setting change; GelGreen™ replaces SYBR® dyes with no optical setting change.
10000 times concentrated solution in water.
can be used with a standard transilluminator (254nm to 366nm)
no changes in optical settings compared to Ethidium bromide
GelRed™ can also be used to stain dsDNA, ssDNA or RNA however GelRed™ is twice as sensitive to dsDNA than ssDNA or RNA.
application:Flexible for different procedures: Use in either precast or post electrophoresis gel staining, compatible with common downstream applications like cloning and sequencing.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-03
Dokumente - Protokolle - Downloads
Here you will find information and further literature on GelRed® in water - DNA staining dye. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.
Dokumente - Protokolle - Downloads
Tips for GelRed ™ applications (gels: 1.5% agarose)
We recommend the in-slot application of a 20X GelRed™-sample mixture.
1. GelRed™ in-slot application:
Dilution of the 10000X GelRed™ stock solution:
3μL GelRed™ (10 000X) are diluted in 97μL H2O or 6X loading buffer. This results in a 300X GelRed™ solution.
8μL of the 300X GelRed™ solution are pipetted into 112μL 6X loading buffer. This results in a 20X GelRed™ working solution.
From the working solution 1μL to 2μL are added to 4μL sample (PCR approach) and applied directly into the slot of the agarose gel.
To evenly fill the gel pockets, more 6X loading buffer can be added, ensuring even filling of the slots. The actual amount depends on the individual experience of the user and should be adjusted individually.
2. Precast gels (GelRed™ in gel):
10μL GelRed™ is recommended per 100mL TBE / TAE gel. It is possible to reduce the GelRed™ volume down to 3.5μL / 100mL gel (it hass to be tested if the intensity of the DNA bands is still sufficient).
3. Post staining:
3X staining solution in water with 0.1M NaCl (addition of NaCL increases sensitivity).
15μL GelRed™ 10000X stock solution are added to 5mL NaCl plus 45mL destilled water.
Leave gel in dyebath for approx. 30 minutes, normal UV illustration.
Don't use TAE / TBE buffer for the dyeing bath solution!
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
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The KISS1 Receptor as an In Vivo Microenvironment Imaging Biomarker of Multiple Myeloma Bone Disease
Julia Dotterweich, Robert J. Tower, Andreas Brandl, Marc Müller, Lorenz C. Hofbauer, Andreas Beilhack, Regina Ebert, Claus C. Glüer, Sanjay Tiwari, Norbert Schütze, Franz Jakob
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J Clin Invest. 2015 Feb 2; 125(2): 593–606. Published online 2015 Jan 2. doi: 10.1172/JCI77780
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Julia Dotterweich, Regina Ebert, Sabrina Kraus, Robert J Tower, Franz Jakob, Norbert Schütze
Cell Commun Signal. 2014; 12: 36. Published online 2014 Jun 25. doi: 10.1186/1478-811X-12-36
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PLoS One. 2013; 8(2): e50695. Published online 2013 Feb 1. doi: 10.1371/journal.pone.0050695
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Elena Rosengauer, Heiner Hartwich, Anna Maria Hartmann, Anya Rudnicki, Somisetty Venkata Satheesh, Karen B. Avraham, Hans Gerd Nothwang
PLoS One. 2012; 7(11): e49503. Published online 2012 Nov 12. doi: 10.1371/journal.pone.0049503