Advantages at a glance
- Quantification of Legionella via qPCR
- Lyophilized Legionella DNA (1.2x 10E6 GU)
- Enabling calibration of the qPCR from 1 GU up to 10E6 GU!
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Product information "Bio Lp-1 Legionella Quantification DNA"
Legionella DNA for Quantification of Legionella via qPCR with the Genaxxon bioscience Bio Lp-1 Legionella Detection Kit.
1 tube of lyophilized Legionella DNA (1.2x 10E6 GU)
1 tube rehydration buffer
The supplied control DNA enables calibration of the qPCR from 1 GU up to 10E6 GU!
For Legionella quantification in routine operations the following are particularly relevant:
• Section 9.3 (Verification of the calibration function of the quantitative PCR phase) and the associated explanations regarding the calibration curve (see also Annex C);
• the requirement for a working calibration range with at least four concentration levels (e.g. 25, 250, 2 500 and 25 000 GU per PCR reaction), whereby the first point in the calibration series should correspond to the limit of quantification (LQqPCR);
• the evaluation using the calibration function (Ct versus log10 of genome units) in accordance with the criteria specified therein regarding linearity, efficiency and accuracy.
Note: A dilution series in triplicate with at least three (preferably four) points meets these requirements and ensures the precision of the reported GU values in routine and official testing.
Specifications:
1 tube with Legionella DNA (1.2x 10E6 GU)
1 tube rehydration buffer
Application:
water test on legionellaClassification:
Documents:
ProtocolsLegionellen-Quantifizierung im Routinebetrieb
Relevant sind hier insbesonders:
• Abschnitt 9.3 (Verification of the calibration function of the quantitative PCR phase) sowie die zugehörigen Ausführungen zur Kalibrationskurve (vgl. auch Annex C);
• die Anforderung an einen working calibration range mit mindestens vier Konzentrationsstufen (z. B. 25, 250, 2 500 und 25 000 GU pro PCR-Ansatz), wobei der erste Punkt der Kalibrationsreihe der Quantifizierungsgrenze (LQqPCR) entsprechen soll;
• die Auswertung über die Kalibrationsfunktion (Ct gegen log10 der Genom-Einheiten) unter den dort genannten Kriterien zu Linearität, Effizienz und Genauigkeit.
Eine Verdünnungsreihe im Triplikat mit mindestens drei, besser vier, Punkten entspricht diesem Rahmen und sichert die Präzision der berichteten GU-Werte im Routine- und amtlichen Betrieb.
Accessory Items
The Legionella Detection Multiplex qPCR Assay is designed to target specific and unique DNA sequences within the Legionella genome. The PCR products from 4 different reactions can be detected by specific DNA probes labeled with 4 different fluorophores. This allows the simultaneous detection of all the Legionella species associated with infection, all the L. pneumophila serogroups (1-16) and specifically identifies L. pneumophila sg-1, responsible for ~95% human infections. An internal amplification control is also included in the master mix as an indicator that the PCR is functioning correctly.Designed to work on the leading real-time PCR platforms, Bio Lp-1 rapidly detects extremely low levels of Legionella, and can differentiate between Legionella spp, all L. pneumophila serogroups 1-16 and L. pneumophila-serogroup 1 simultaneously in a single water sample, giving a complete and accurate Legionella profile. With this certainty, the exact risk to public health can be quickly determined and acted upon. Particularly beneficial in cases of an outbreak, appropriate remedial actions can be taken, their efficacy monitored to reduce and remove Legionella bacteria from a contaminated water system. In less than 5 hours, water quality and safety can be assured. Simple and convenient to use, and in a freeze-dried format, the test contains all necessary components to process a water sample directly. Bio Lp-1-Legionella multiplex one step qPCR Kit is a diagnostic breakthrough, being the world’s first open platform rapid NAD kit for simultaneously detecting and identifying multiple species of Legionella from water in a single test. The kit comes together with negative and positive DNA control enabling to prepare standards of 10E5 GU, 10E4 GU, 10E3 GU, 10E2 GU, 10E1 GU (with 1 GU = one genomic unit). TACT US > TO GET A TEST SAMPLE LEARN MORE > GET MORE INFORMATION