Advantages at a glance
- Assay (Protein): min. 85%
- Activity (dried basis): (KIU/mg): min. 5250 (3.73 TIU/mg)
- pH (1%; H2O): 5,0 - 7,5
- Water (K.F.): max. 10%
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Product information "Aprotinin from bovine lung"
Description:
Aprotinin is mainly obtained from bovine lung. Aprotinin consists of 58 amino acids of 6511 Da and is very basic, whereby amino acids 13-18 of Aprotinin are of particular importance for binding to serine proteases. As Aprotinin has been described by various research groups, two names have become established: 1. bovine pancreatic trypsin inhibitor (BPTI) and 2. trypsin-kallikrein inhibitor (TKI). Aprotinin is often used as a non-specific serine protease inhibitor, especially for inhibition of Trypsin.
Effect:
Aprotinin inhibits proteases such as Trypsin, Plasmin, Chymotrypsin and Thrombin.
Aprotinin is a competitive inhibitor of serine proteases that forms stable complexes with the enzyme and blocks active enzyme sites. These bindings are reversible and most aprotinin-protease complexes dissolve at very low or high pH values (<3 and >10).
Application:
Aprotinin is mainly used as a trypsin inhibitor.
Stability:
Aprotinin can be stored in lyophylised form at +2°C to +8°C for an unlimited period of time. In salt or buffer solutions (pH 5 - 8), Aprotinin is stable for at least one month at +2°C to +8°C or for several years at -20°C. Stock solutions (e.g. 10mg/mL) should be frozen in small aliquots and used only once to avoid repeated freezing and thawing.
Unit definition:
One trypsin inhibitory unit (TIU) inhibits the activity of 2 trypsin units by 50%, whereby one trypsin unit hydrolyses 1 μmol Nα-benzoyl-DL-arginine-p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C. 1 TIU (trypsin inhibitory unit) corresponds to approx. 1300 KIU (kallikrein inhibitory units) and 1 TIU corresponds to one Ph. Eur. unit.
Assay (Protein): min. 85%
Activity (dried basis): (KIU/mg): min. 5250 (3.73 TIU/mg)
pH (1%; H2O): 5,0 - 7,5
Water (K.F.): max. 10%
Formula: C284H432N84O79S7
MW: 6511,52 g/mol
Application:
Inhibition of Serin proteasesUnits:
One trypsin inhibitory unit (TIU) inhibits the activity of 2 trypsin units by 50%, whereby one trypsin unit hydrolyses 1 μmol Nα-benzoyl-DL-arginine-p-nitroanilide (BAPNA) per minute at pH 7.8 and 25°C.Source:
bovine lungClassification:
Documents:
CertificateGeneral Data 1
Source: NCBI PubMed