Advantages at a glance
- Pre-mixed buffer powder with pre-set pH
- Easiness: Eliminates calculation, formulation and weighing errors
- Dissolve-and-go for greater convenience
- Stability: Stable at room temperature for at least 3 years
- Guaranteed reproducibility
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Product information "PBS buffer tablets (pH7.4) with Tween(R)20"
Precisely weighed ready-to-use PBS tablets with Tween®20 offered in plastic bottles or in practical blister packs.
- no awkward weighing anymore
- no toilsome pipetting/weighing of Tween®20 anymore
- no more time-consuming pH adjustment
- simply dissolve the tablet completely in demineralized water and use the buffer
Ready-to-use PBS buffer tablets with Tween®20 containing the non-ionic detergent Tween®20 which acts through blocking. It has the ability to reduce non-specific binding and protein-protein interaction during the wash step in protein and immunoassay procedures such as ELISA and Western blotting. By decreasing the non-specific binding and staining makes interpretation of ELISA results and Western blots easier.
Each tablet dissolved in deionized water yields a 0.14M sodium chloride 0.0027M potassium chloride, 0.05% Tween®20, 0.01M phosphate buffer with pH7.4 at 25°C. Final buffer volumes are: 100mL and 500mL (D2003 >) or 1L (D2004 >).
Our ready-to-use tablets are also available as PBS tablets without Tween®20, as TBS or as IMAC tablets for affinity chromatography.
Other phosphate-based ready-to-use buffers:
PBS without Potassium (0.01M) (D2033 >).
PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >).
Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >
Specifications:
Purity of used chemicals: min. 99.0%
0.01M Phosphate buffer
0.0027M KCl
0.14M NaCl
0.05 % Tween™20
pH7.4 at 25°C (final buffer solution)
Tablets for 100mL or 500mL final volume.
Application:
- Wash buffer in immunolabelling techniques, such as ELISA and Western blotting- Blocking buffer for plate based assays- Protein-plate coatingSource:
syntheticClassification:
Documents:
Safety Data SheetCertificate
Category List
Source: NCBI PubMed
Camille M H Clément, Maika S Deffieu, Cristina M Dorobantu, Thomas F Baumert, Nilda Vanesa Ayala-Nunez, Yves Mély, Philippe Ronde, Raphael Gaudin
Biol Cell. 2020 May;112(5):140-151.
doi: 10.1111/boc.201900085.
PMCID: PMC7613415.