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Product information "Ready-to-use Citrate buffer of pH4.5 - 0.1M"
Citrate Buffer (Citric acid/ Sodium citrate), pH 3.0–6.2
A citrate buffer is a buffer solution consisting of citric acid and sodium citrate, which maintains a stable pH within the acidic to neutral range (typically pH 3.0–6.2). We supply ready-to-use buffer mixtures for standard pH values as well as customised citrate buffers tailored to your application.
Key Properties
- Buffer Range: pH 3.0–6.2 (maximum buffer capacity close to the pK_s values of citric acid: pK₁ ≈ 3.1, pK₂ ≈ 4.8, pK₃ ≈ 6.4)
- Typical concentrations: e. g. 0.1M ffor aqueous biochemical/molecular biology buffers; 0.5% (w/w) powder content for cosmetic DAC/NRF formulations.
- Availability: Pre-set pH values (e.g. 3.0–4.5) in stock; other pH ranges and concentrations available on request.
Typical applications
- Biochemistry & Molecular Biology: investigation of pH-dependent enzymes (e.g. acid phosphatases), protein elution, RNA isolation.
- Cosmetics & Pharma: adjustment to a skin-friendly pH value (often pH 4.2–4.6, within the range of the skin’s natural pH of approx. 4.5–5.5).
Custom made citrate buffers tailored specifically to your application
For development and production projects, we offer bespoke citrate buffer formulations with customised parameters (target pH, citrate concentration, required buffer capacity, batch size). Simply get in touch to enquire: info@genaxxon.com
Application:
Herstellung biologischer Puffer. Puffer für kosmetische Pasten und Cremes.Classification:
Documents:
DNA Ladepuffer
Coral Red Buffer is a 10-time dye solution as supplement for PCR buffers. This additive contains Glycerol, EDTA, a red and a yellow dye. 5µL of this solution are added to 45µL of the PCR assay before the PCR reaction to stain it red. An alternative to this product is the Genaxxon bioscience ready-to-use RedMastermix M3029. The RedMastermix contains all incredients necessary for PCR including a red dye that enables to control the different pipetting steps.
Gel Loading Dye I 6X is a loading buffer for DNA application on gels. Buffer contains Glycerol, EDTA, Bromophenol Blue and Xylene cyanol. Genaxxon 6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis.
Loading buffer I Fluoro is a fluorescent reagent that produces instant visualization of DNA bands upon blue light or UV illumination of agarose gels. Supplied in 6X DNA Loading Buffer, Loading buffer I Fluoro is used to prepare DNA markers or samples for loading on agarose or polyacrylamide gels. Loading buffer I Fluoro is the sensitive staining reagent available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (bromophenol blue, xylene cyanol FF, and orange G) for visually tracking the DNA migration during the electrophoresis plus an additional fluorescence dye for detection of DNA bands without an additional staining of the gel. Please note: DNA Loading Buffer I Fluoro does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation (ca. 490nm). We therefore recommend using blue light to achieve the best possible results.
DNA 6X Loading buffer is a special loading buffer for DNA application on gels that enables viusalization of DNA bands smaller than 100 bp without problems. Buffer contains Glycerol, EDTA, Orange G. The special dye gives the opportunity to detect even DNA bands running below 65 bp. Other loading dye: M3308 and PCR dye: M3309.