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Product information "BCIP, molecular biology grade"
BCIP (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase, used in combination with the oxidant NBT (nitro blue tetrazolium) to enhance blue color development.
Applications:
When alkaline phosphatase conjµgates are used for detection, BCIP is recommended for the following applications: Southern blotting, Western blotting, Northern blotting and dot/slot blots.
It is recommended to prepare a 50mg/mL stock solution in DMF.
Protocol for Preparation of BCIP/NBT Substrate Solution:
Add the following components
- 10mL of the Alkaline Phosphatase (AP) buffer (100mM Tris-HCl (pH9.5), 100mM NaCl and 10mM MgCl2)
- 33µL BCIP (50mg/mL in DMF)
- 44µL NBT (75mg/mL in 70% DMF).
NOTE: Avoid exposure to light - Prepare fresh developing solution and use within an hour - BCIP and NBT solutions in DMF do not freeze. They are stable for approximately 2 years when stored at -20°C in the dark.
Specifications
Assay: min. 98% (HPLC)
pH (5% in water): 1.0-2.0
Solubility (water, 20°C): 582 g/L
MP = 120°C
Chloride: max. 0.001%
Pb: max. 0.005%
Free acid: max. 0.1%
C8H6BrClNO4P x C7H9N
MW = 433.64 g/mol
Application:
Southernblotts, Westernblotts, Northern blotting und dot/slot blots.Source:
syntheticSafety Information:
Classification:
Documents:
Safety Data SheetCategory List