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Product information "Rose-Gal / 6-Chloro-3-indolyl-b-D-galactopyranoside"
Rose-Gal, also known as Red-Gal or Salmon-Gal, (6-Chloro-3-indolyl-ß-D-galactopyranoside), is a is a beta-galactosidase substrate which produces a salmon pink colour. Rose-Gal (Red-Gal / Salmon-Gal) serves as an alternative to X-Gal > for the enzymatic detection of Lac+ beta-Galactosidase activity in bacterial colonies. In colorimetric assays, recombinants (white) can be distinguished from non-recombinants (salmon). In conjunction with X-Gluc >, Rose-Gal (Red-Gal, Salmon-Gal) can be used for the simultaneous detection of GUS and Lac + activity on the same plate.
Specifications:
Purity: min. 98.5%
Appearance: Off-white to pale pink powder
Solution: Clear, colourless to pale pink solution
Spec. rotation (a20°C/D; c=1, MeOH): -46°+/-4°
Water (K.F.): <1%
C14H16ClNO6
MW = 329.73 g/mol.
Application:
Used for detection b-glucoronidase in different tissue samples and of E.coli in food samples.Source:
syntheticClassification:
Documents:
Category ListSource: NCBI PubMed
Accessory Items
X-Gal (5-Brom-4-chlor-3-indolyl-β-D-galactopyranosid) is a chromogenic substrate for β-galactosidase. It produces a blue color that can easily be detected visually. X-Gal is used in the identification of lacZ + bacteria, especially for the detection of ß-galactosidase, which is expressed by the recombinant vectors. X-Gal is often used in conjunction with IPTG > (for the induction of the lac promoter (lac operon)), for the screening of blue colonies. It is also used for the screening of ß-galactosidase reporter gene activity in transfections of eukaryotic cells. X-Gal is a substrate of ß-galactosidase, which releases by cleavage of the substrate 5-bromo-4-chloro-3-indoxyl (X). The enzymatically cleaved indoxyl is oxidized to the insoluble indigo. As a stock solution, for example, a 20mg/mL X-Gal solution in dimethylformamide can be prepared. For the use in LB / Amp - plates this stock solution is to be diluted 1 : 1000 (final concentration 20μg/mL) or 1: 500 (final concentration 40μg/mL). The heated medium should thereby be cooled to about 50°C. The amount of X-Gal may be reduced if an X-Gal solution in DMF is sprayed to the agar plates (already with bacterial colonies!). X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.