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Product information "p-Nitrophenylphosphate (5mg) tablets"
24 respective 100 ready-to-use para-Nitrophenylphosphate (pNPP) tablets with 5mg p-Nitrophenylphosphate. Each tablet dissolved in 5mL deionized water yields a solution containing 1.0mg/mL pNPP, 0.2M Tris buffer.
Genaxxon's pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays. Its soluble end-product is yellow and can be spectrophotometrically read at 405 to 410 nm. The reaction may be stopped with 3M NaOH.
The pNPP substrate is supplied as pre-weighed tablets in bottles or in convenient blister packs, each tablet containing 5mg or 20mg of substrate.
How to use:
Deposit one tablet of the pNPP substrate in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water in the appropriate amount to reach the required concentration for the assay. Stir until full dissolution and the substrate solution is ready to use.
One tablet dissolved in 5mL of substrate buffer results in a 1mg/mL solution.
Specifications:
tablets with 5mg p-Nitrophenylphosphate
Weight of tablet: 50mg +/- 1mg
Content: 5mg pNPP +/-1mg
Appearance: white to sligthly yellow tablets
Application:
Immunoassays, Chromogenic substrate for phosphatase assays, Histochemistry procedures, Recommended for ELISA proceduresSource:
syntheticClassification:
Documents:
Safety Data SheetCertificate
Product description
Category List
Common buffers used in ELISA tests:
a. 0.1M glycine, pH10.4, 1mM MgCl2, 1mM ZnCl2.
b. 1M diethanolamine, pH9.8, 0.5mM MgCl2.
c. 0.2M Tris, pH8.8, 5mM MgCl2.
Preparation of a sterile solution:
- Prepared solution may be sterilized by filtration (0,2µm). The solution has to be stored at -20°C in aliquots!
Source: NCBI PubMed
Accessory Items
24 respective 100 ready-to-use p-Nitrophenylphosphate (pNPP) tablets with 20mg p-Nitrophenylphosphate. Each tablet dissolved in 20mL deionized water yields a solution containing 1.0mg/mL pNPP, 0.2M Tris buffer. Genaxxon's pNPP substrate is specifically developed for immunoassay procedures and it is ideal for phosphate-based ELISA methods. The substrate is also used as a chromogenic non-specific substrate in alkaline and acid phosphatase assays. Its soluble end-product is yellow and can be spectrophotometrically read at 405 to 410 nm. The reaction may be stopped with 3M NaOH. The pNPP substrate is supplied as pre-weighed tablets in bottles or in convenient blister packs, each tablet containing 5mg or 20mg of substrate. How to use:Deposit one tablet of the pNPP substrate in a laboratory flask or beaker placed on a magnetic stirrer. Add deionized water in the appropriate amount to reach the required concentration for the assay. Stir until full dissolution and the substrate solution is ready to use. One tablet dissolved in 20mL of substrate buffer results in a 1mg/mL solution.