3 reasons why you should use the novel Taq DNA polymerase in Genaxxon’s 5X qPCR Multiplex MasterMix for multiplex qPCRs

#1: The novel and improved DNA polymerase is optimally designed for robust and fast multiplex qPCR. 

No doubt you know about the relevance of choosing the right DNA polymerase for a robust and reproducible qPCR. After all, the specificity and sensitivity of your qPCR can be severely impaired by undesired DNA synthesis. The novel Taq DNA polymerase of the 5X qPCR Multiplex MasterMix from Genaxxon bioscience has been specifically developed to meet these challenges. 

Read here the most important advantages of the novel Taq DNA polymerase: 

• Reduced PCR run time: Since no activation step is necessary, the PCR run time can be significantly shortened. 
• Inactive at room temperature: Thanks to a mutation in the α-helix 28 of the Taq DNA polymerase, activation of the enzyme only occurs at optimal PCR cycle temperatures. 
• Improved hot start activity: A specifically designed aptamer sequence binds to the active site of the Taq DNA polymerase and guarantees reversible thermal blocking. 
• Robust and uniform amplification: The newly developed Taq DNA polymerase offers high sensitivity and specificity for multiplexing. Thus, it has already been successfully used for the amplification of up to 30 different DNA sequences in a single reaction. 

#2: Fast and easy: Direct use of blood and plasma samples saves time and costs. 

The 5X qPCR Multiplex MasterMix from Genaxxon bioscience can also be used directly with impure samples such as blood and plasma. Time-consuming purification steps can be omitted for many applications, as the specially developed Taq DNA polymerase has a significantly higher tolerance to common PCR inhibitors than other DNA polymerases. This allows you to save time and costs to improve efficiency. 

#3: Increase your flexibility thanks to possible lyophilisation. 

The specially developed Taq DNA polymerase also allows use in a lyophilised form. The 5X qPCR Multiplex MasterMix from Genaxxon bioscience does not contain any glycerol, so you can easily perform the lyophilisation yourself without adding any other excipients. The lyophilised form is consistently efficient, but also offers the advantages of molecular biological assays with lyophilised reagents. For example, storage can be at room temperature, shelf life is significantly extended and your flexibility in design and sample volume is increased. You only need to add primers and probes - saving time and costs in assay preparation. 

Use Genaxxon’s 5X qPCR Multiplex MasterMix now for your next multiplex qPCR. Do you have further questions? Contact us here or send a mail to: info@genaxxon.com