NGSClone PCR Mastermix – High-Fidelity PCR for Demanding Applications

August 21, 2025 I. Steitz
Science High Fidelity Polymerase, Proofreading Polymerase, HiFi Polymerase, NGS, Next Generation Sequencing

NGSClone PCR Mastermix – High-Fidelity PCR for Demanding Applications

In modern molecular biology, precision PCR is essential – especially for NGS library preparation, GC-rich template amplification, and error-free cloning. The Genaxxon NGSClone PCR Mastermix (2x) combines a highly accurate high-fidelity DNA polymerase with Hot-Start technology in a ready-to-use format.

It delivers performance comparable to modern proofreading polymerases such as Phusion®, while offering broad template tolerance, robust performance, and reliable results.

Maximum accuracy with proofreading activity

The polymerase in the NGSClone PCR Mastermix (2x) features strong 3’→5’ exonuclease proofreading activity and achieves approximately 100-fold higher fidelity than standard Taq polymerases. This makes it the ideal choice for applications where sequence accuracy is critical – from error-free cloning to high-fidelity NGS PCR.

Hot-Start technology for high specificity

The antibody-mediated Hot-Start system keeps the polymerase inactive at room temperature, activating only at the start of the PCR cycle. This minimizes non-specific amplification and increases reproducibility – even with complex or challenging templates.

Versatile applications

The NGSClone PCR Mastermix (2x) is optimized for a wide range of demanding PCR workflows, including:

High-fidelity PCR for cloning
GC-rich DNA amplification
cDNA PCR
NGS library preparation
Standard PCR with maximum accuracy

With high processivity, it enables fast amplification at only 10–15 seconds per kilobase.

Laboratory efficiency and stability

As a ready-to-use master mix, NGSClone PCR Mastermix (2x) saves time, reduces pipetting steps, and lowers the risk of cross-contamination. The formulation remains stable even after repeated freeze–thaw cycles – and additionally demonstrates exceptional temperature stability: at 37 °C, the master mix remains stable for at least one week. This ensures maximum flexibility in everyday laboratory workflows.

Conclusion

The Genaxxon NGSClone PCR Mastermix (2x) combines highest fidelity, robust stability, and user-friendly handling in a single product. It is the ideal choice for researchers who demand uncompromising precision in challenging applications such as NGS library preparation or error-free cloning.