January 15, 2024
I. Steitz
Science
Science
qPCR FAST and Furious: Turbo charge your qPCR applications
By using special instruments and optimized master mixes aimed at speed and efficiency, this technology allows you to accelerate your qPCR applications without compromising on specificity and sensitivity.
When time is precious: switch to FAST-qPCR
We all know this situation: deadlines are approaching, samples need to be processed urgently and the thermal cycler is constantly busy. Even if you have got hold of a short timeslot in the busy equipment calendar, you are faced with the next problem: with a standard run time of 2 hours, a maximum of 4 qPCR runs can be carried out in an 8-hour slot. This limits the number of possible samples from the outset. In the event of unforeseen problems and repetitions, the stress level increases enormously. But is there a solution?
Switching to FAST-qPCR: Turbo for your throughput
Switching to FAST-qPCR could be the answer. It allows you to double or even triple your throughput without additional equipment investment. While a standard qPCR takes about 120 minutes, using a FAST-qPCR master mix enables the same run in an impressive 40 minutes. Even if your thermal cycler is not "FAST-capable", you can still achieve a time saving of 50% - the same results in just 60 minutes.
How is this possible?
Since the discovery of PCR and the development of the first thermocycler in the 1980s, the methods and equipment used have been continuously refined. Just remember that before the discovery of Taq polymerase, you had to add a new enzyme to each PCR cycle - an effort that sounds completely absurd in today's laboratory routine. The polymerases used, as well as the other PCR reagents and thermal cyclers, have therefore been continuously developed in line with the motto "faster, more efficient, more convenient" in order to meet the requirements of the modern PCR laboratory. The FAST-qPCR is an even further major step towards greater efficiency in the laboratory. With a FAST-capable thermal cycler, the ramping rate can be significantly steeper thanks to improved thermoregulation and optimization of other device-specific factors, thus shortening cycle times.
But what if your laboratory only has a standard thermocycler?
Don't worry! Using a FAST thermal cycler is not the only way to save time in the qPCR lab. Thanks to the further development and optimization of qPCR reagents, you can also run significantly faster cycle times with a standard thermal cycler and thus effectively save time. Genaxxon's FAST-qPCR master mixes have been specially developed and optimized for FAST-qPCR. This means that you can obtain your qPCR results in just 40-60 minutes, even without a FAST-capable thermal cycler.
Save time and money with Genaxxon's FAST-PCR master mixes
The use of FAST-qPCR master mixes not only reduces your time, but also your costs. Optimized for comparable or even better performance compared to conventional PCR approaches, these reagents allow efficient use of samples and reagents at lower volumes. This is not only economical, but also opens up opportunities for users with budget constraints to increase their daily throughput capacity.
Multiplexing for ultimate flexibility
For particularly demanding applications, Genaxxon offers various multiplex master mixes, such as the 5X qPCR Mastermix. Thanks to its specifically optimized DNA polymerase, it offers ultimate flexibility for multiplexing scenarios. This innovative solution not only enables the simultaneous amplification of different targets, but also saves time through parallel amplification. An asset for challenging research projects that require maximum efficiency.
Conclusion: Fast, Efficient, Reliable - the Future of PCR
FAST-qPCR technology has changed the rules of the game in the world of qPCR applications. FAST-qPCR master mixes and thermal cyclers are powerful tools that not only save time but also deliver reliable results. A look into the future of qPCR shows an acceleration of research and diagnostics - FAST and furious!
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