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Product information "MEM Amino Acids Solution (50X)"
Genaxxon MEM Amino Acids Solution is a 50-time concentrated solution of all essential amino acid with the exception of L-glutamine. This supplement is used to increase cell growth and viability of cells in cell culture. Genaxxon MEM Amino Acids Solution is formulated to contain 50X the essential amino acids (except L-glutamine) found in the standard Minimum Essential Medium (MEM). The complete formulation is available.
This product is for laboratory usage only.
Genaxxon offers also L-glutamine as a ready-to-use 200mM concentrated solution (C4281 >).
Specifications:
L-Arginine hydrochloride: 6320.00 mg/mL
L-Cystine: 1200.00 mg/mL
L-Histidine hydrochloride - H2O: 2100.00 mg/mL
L-Isoleucine: 2620.00 mg/mL
L-Leucine: 2620.00 mg/mL
L-Lysine hydrochloride: 3625.00 mg/mL
L-Methionine: 755.00 mg/mL
L-Phenylalanine:1650.00 mg/mL
L-Threonine: 2380.00 mg/mL
L-Tryptophan: 510.00 mg/mL
L-Tyrosine: 1800.00 mg/mL
L-Valine: 2340.00 mg/mL
Application:
amino acid and vitamine liquid supplements for cell cultureClassification:
Documents:
Accessory Items
200mM L-Alanyl-L-Glutamine solution (100-times) (stabile Glutamine) is a ready-to-use, sterile 200mM cell culture media supplement. The concentration of L-glutamine used in classical media ranges from 0.5mM in Ames' Medium to 10mM in MCDB Media 131. The more typical concentrations in media used is between 2 and 6mM. DMEM/F12 (50:50) is often used as a starting formulation for media used with Chinese Hamster Ovay, CHO, cells. L-glutamine in DMEM/F12 is 2.5mM. L-glutamine in DMEM, GMEM, IMDM and H-Y medium is 4mM.Normal L-Glutamin is not stable in solution. A drawback of the spontaneous breakdown once in solution is the generation of ammonia as a by-product, which is toxic to the cells and can affect protein glycosylation. One strategy to minimize generation of ammonia is to replace the L-glutamine in medium with stable glutamine (L-Alanyl-L-Glutamine) from Genaxxon. This supplement shows an improved stability compared to free L-Glutamine and considerable reduction of spontaneous ammonia generation. Stable glutamine is stable for 24 months at room temperature, +2 to +8°C or -20°C, thus ensuring user-friendly storage and operation.
N-acetyl-L-alanyl-L-glutamine (stable glutamine) for cell culture. L-glutamine is an essential amino acid that is essential for cell growth. While normal L-glutamine undergoes autohydrolysis and is therefore not stable in solution, it can be substituterd by the addition of stable N-acetyl-L-alanyl-L-glutamine which guarantees cell culture medium still containing "L-Glutamine" even after 2 years storage. N-acetyl-L-alanyl-L-glutamine is cleaved by cellular aminopeptidases into alanine and glutamine, providing the cell with the necessary supply of L-glutamine for growth. Mechanism:When cells are cultured in N-acetyl-L-alanyl-L-glutamine containing media, the cells gradually release aminopeptidases which hydrolyze the dipeptide, slowly releasing L-alanine and L-glutamine into the culture medium. L-glutamine and L-alanine can then be taken up by the cells and used for protein production or in the TCA cycle. This mechanism can be compared to the fed-batch culture strategy in which L-glutamine is continuously fed into the culture but kept at a low concentration. The result is an efficient energy metabolism and a high growth yield without excess ammonia.
L-alanyl-L-glutamin (stable Glutamine) for use in cell culture.