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UCMSC Exosome HY Medium

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Product information "UCMSC Exosome HY Medium"

Overview:

The UCMSC Exosome HY Medium is a specially designed serum-free and ready-to-use culture medium for the isolation and cultivation of exosomes from umbilical cord-derived mesenchymal stem cells (UCMSC). This medium promotes efficient exosome production and ensures optimal conditions for their collection and application in research and therapy.

Product Details:

Formulation: Specifically tailored for the cultivation of umbilical cord-derived mesenchymal stem cell and the isolation of exosomes. No need to add common nutritional supplements such as serum or human platelet lysate; however, users can decide to add nutritional supplements based on actual needs.
Components: Contains all necessary nutrients, growth factors, and supplements to ensure healthy cell growth and high exosome production.
Application: Ideal for research applications, including cell communication studies, regenerative medicine, and therapeutic developments.
Compatibility: Works excellently with standard cell culture protocols and equipment. 
Stability: The medium is stable at +2°C to +8°C for 2 years.

Benefits: 

Efficient Exosome Production: Optimized formulation promotes high exosome yield. 
Quality and Consistency: Consistent results through standardized ingredients and production. 


Application:

For exosome production, we recommend culturing UCMSCs in dishes or flasks under regular culture conditions up to 90% confluence. Then remove the original culture medium and wash the cells with PBS and  Exosome HY medium to remove residues of the original medium. Then the  cells are cultured in  Exosome HY medium for 3-4 days before the conditioned medium can be used for exosome isolation.


Picture 1: Nanoparticle Tracking Analysis: Size verification

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Picture 2: Yield comparison between standard cell culture media and UCMSC Exosome HY Medium
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Picture 3: Flow Cytometry - Verification of CD9, CD63, CD81 surface marker

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Produced by Genaxxon bioscience GmbH, founded in 2002 by Dr. Norbert Tröndle to provide reliable products for PCR and custom PCR and cell culture media formulations, ADSC Exosome HY Medium offers a dependable and efficient solution for your cell culture and exosome research needs. Optimize your experiments and achieve consistent, high-quality results with a medium designed specifically for your requirements.


Conventional Methods for Exosome Extraction and Isolation


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Workflow with Magnetic Beads for Exosome Purification


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Specifications:
with 4.5g/L D-Glucose
with 4.0mM L-Glutamine
with 25mM HEPES
without Phenol Red

Application:

High yield exosome isolation

Classification:

eclass no.: 32-16-08-01
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Safety Data Sheet
Certificate
Product description

Exosome Isolation and Purification

Exosomen: Optimieren Sie Ihre Exosomen-Isolation - Isolationstechniken im Überblick - Membranaffinität - Solid-phase binding - Magnetic Beads

Exosomes: Intimate Mail to Cells

Exosomes: powerful nanoparticles in intercellular communication