Advantages at a glance
- 2.5% Trypsin in PBS
- Without EDTA
- Without Ca2+ and Mg2+
- Without Phenol red
- Mycoplasma tested for use in cell culture
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Product information "2.5% Trypsin in PBS (10X)"
Trypsin is a mixture of proteases isolated from porcine pancreas. 2.5% Trypsin is produced by dilution of Trypsin in PBS. Due to its protease activities, trypsin solutions are widely used for cell dissociation, routine cell culture passaging, or primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. For this Genaxxon offers a variety of different Trypsin solutions with and without EDTA.
Activity of the used Trypsin: 1g of Trypsin digests 250g of Casein substrate.
Minimal order size: 5 x C4287.0100 (100mL) or 2 x C4287.0500 (500mL).
Specifications:
2.5% Trypsin in PBS
without EDTA
without Ca2+ and Mg2+
without Phenol red
mycoplasma tested for use in cell culture
Application:
enzyme for cell dissociation and for hydrolytic degradation of proteins and peptidesUnits:
1 unit catalyses the hydrolysis of 1 µmol N-a-benzoyl-L-arginine ethyl ester (BAEE) per minute at 25°C, pH 8.0.Classification:
Documents:
Safety Data SheetCertificate
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Accessory Items
Accutase® as a cell detachment solution of proteolytic and collagenolytic enzymes useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue. It is much better suited for cell detachment than trypsin. Proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Accutase® does not contain mammalian or bacterial derived products. For that reason Accutase® virus or endotoxin contaminations are excluded. Once thawed Accutase® can be used for about 1 week if stored at +2°C to +8°C. Shelf-life at -20°C is 12 months. Note: Accutase is a registered trademark of Innovative Cell Technologies, Inc.