Advantages at a glance
- cell permeable fluorescent dye
- binds selectively to DNA
- binds to the minor groove of double-stranded DNA
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Product information "DAPI - 4',6-Diamidino-2-phenylindole dihydrochloride, min. 98%"
4',6-Diamino-2-phenylindole hydrochloride is a fluorescent dye binding selectively to the minor groove of double strand DNA (preferentially to AT rich DNA). Forms a stable complex which fluoresces approximately 20 times more than DAPI alone.
DAPI, once added to tissue culture cells, is rapidly taken up into cellular DNA, yielding highly fluorescent nuclei and no detectable cytoplasmic fluorescence.
When the cells are contaminated with Mycoplasmas, characteristic discrete fluorescent foci are readily detected over the cytoplasm and sometimes in intercellular spaces. Also used for flourescent chromosome staining or to stain brain stem cells.
Preparation of solutions:
Stock solution: Dissolve in distilled water to a final concentration of 1 to 5mg/mL. NOTE: Do not use any buffers. The stock solution is stable at -15°C to -25°C for about 12 months.
Working solution: Dilute the stock solution with methanol to a final concentration of 1μg/mL. The working solution is stable at +2 to +8°C for about 6 months.
Specifications:
Purity: min. 98% (HPLC)
molecular biology grade
fluorescence excitation: 342 nm
fluorescence emission: 450 nm
Solubility (1%, H2O),clear
Nitrogen: min.18%
Chemical formula: C16H15N5 x 2HCl
MW = 350.3 g/mol;
Stability / Storage conditions:
Stock solution (1 to 5mg/mL) at -15°C to -25°C for 12 months.
Working solution (1μg/mL) at +2°C to +8°C for about 6 months.
Application:
detection of mycoplasmas and viruses flourescence staining of chromosomes staining of brainstem cells.Classification:
Documents:
Safety Data SheetCertificate
Product description
Category List
Accessory Items
HighPure Propidium iodide (3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide) for selective labeling of DNA in dead cells as cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells. Propidium iodide may be used in tumor diagnosis flow cytometry to evaluate cell viability when used with other dyes that stain viable cells or cells that are in early apoptosis process. Can also be used for the study of micronuclear morphology of protozoa. Propidium iodide can be excited by light with wavelenghts in the visible range and bleaches slowly, having clear advantages over DAPI. CAUTION: Potent carcerogenic agent. Valet G. et al. (1987) Blut 49, 37-43. Fluorescent stain for nucleic acids. At a concentration of 1 µg/mL, probably all DNA binding sites are saturated.