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Product information "Ponceau S (C.I. 27195)"
Ponceau S is used for reversible staining proteins blotted on nitrocellulose membran and while running a TAU electrophoresis. The reversibility of the staining process is ideally suited for subsequent immunoblots (western blot) or other colour reactions as Ponceau S can be completely removed. Ponceau S is not stable for long time, thus proteins and protein markers should be marked with a pencil. For staining the membrane is incubated in a 0.1% Ponceau S solution (w/v) in 5% acetic acid for 5 - 10 minutes. Destaining is done by incubating the stained membrane in 5% acetic acid for 15 minutes.
Specifications:
Assay: min. 80%
Appearance: dark red to brown powder
Loss on drying: max. 10%
λ(max) (water): 517-523nm
C22H12N4Na4O13S4
MW = 760.56 g/mol
Application:
for reversible staining of blotted membranesClassification:
EC no:
228-319-2
CAS no.:
6226-79-5
eclass no.:
30191015
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Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Documents:
Safety Data SheetCertificate
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