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Product information "4,5-diaminofluorescein diacetate - DAF-2 DA"
4,5-diaminofluorescein diacetate (DAF-2 DA) is a highly sensitive probe for the real time detection of NO in vivo. Cell permeable.
Application: DAF-2 DA is probably the most successful indicator for nitric oxide. As with other fluorescein diacetates, DAF-2 diacetate is membrane permeant and is deacetylated by intracellular esterases to 4,5-diaminofluorescein (DAF-2). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure realtime changes in nitric oxide levels in vivo.
References:
V.M. Dirsch et al.; Biol. Proc. Online 5, 136 (2003)
N. Nagata et al.; J. Biochem. 125(4), 658 (1999)
H. Kojima et al.; Anal. Chem. 70(13), 2446 (1998)
H. Kojima et al.; Chem. Pharm. Bull. 46, 373 (1998)
H. Kojima et al.; Neuroreport 9, 3345 (1998)
Specifications:
Purity: >98% (HPLC)
Appearance: Off-white powder
Identity: determined by NMR
C24H18N2O7
MW = 446.41 g/mol
Fluorescence: λex 491 nm; λem 513 nm
Solubility: soluble in DMSO
Application:
fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.Source:
syntheticSafety Information:
Classification:
Documents:
Safety Data SheetCertificate
General Data 1
Category List
Source: NCBI PubMed
Ana Salas, Germán Tortosa, Alba Hidalgo-García, Antonio Delgado, Eulogio J Bedmar, David J Richardson, Andrew J Gates, María J Delgado
Front Microbiol. 2020 Jan 10;10:2915.
doi: 10.3389/fmicb.2019.02915.
PMCID: PMC6965051.
Alexandra Kleinknecht, Blagovesta Popova, Diana F Lázaro, Raquel Pinho, Oliver Valerius, Tiago F Outeiro, Gerhard H Braus
PLoS Genet. 2016 Jun 24;12(6):e1006098.
doi: 10.1371/journal.pgen.1006098.
PMCID: PMC4920419.
Accessory Items
5/6-TAMRA is a mixtures of the 5' and 6' isomers of carboxytetramethylrhodamine (TMR) free acid. The non-activated single isomer carboxytetramethyl rhodamine derivative is used as a red fluorescent derivatization reagent for covalently labeling proteins and alkylamino modified nucleic acids. The fluorescence quantum yield of TAMRA conjugates is about one-fourth that of fluorescein conjugates. Nevertheless, TAMRA conjugates often appear brighter than the corresponding fluorescein conjugates due to the fact that TAMRA is readily excited by the 546nm spectral line from mercury-arc lamps used in most fluorescence microscopes and is intrinsically more photostable than fluorescein. TAMRA is also efficiently excited by the 543nm spectral line of the green He-Ne laser. Succinimidyl esters of 5-TAMRA >, 6-TAMRA > or of the mixed 5/6 TAMRA isomers > are the labeling reagents of choice for the preparation of fluorescent bioconjugates, including peptide, protein, nucleotide and nucleic acid conjugates, especially for fluorescent antibodies or avidine derivatives used in immunochemistry. These fluorescent dyes are also used as acceptors for FAM > fluorophores in FRET studies. More Fluorescent Dyes from Genaxxon: 5/6-TAMRA >, 5-TAMRA >, FAM >, ROX >, DAF-2 >.
4,5-Diamino-N,N,N',N'-tetraethyl-rhodamine. Sensitive NO probe, LOD of 10 nM, shows higher photostability than the classical fluorescein derivative DAF.
4,5-Diaminofluorescein triazole (DAF-2T) can be used as a reference material for DAF-2 (S5439 >).References: (1) M. Feelisch et al;, Free Radical Biol. Med. 38(3), 356 (2005); (2) T. Nagano et al.; J. Biol. Chem. 277(1), 47 (2002); (3) T. Nagano et al.; FEBS Letters 427(2), 263 (1998); (4) H. Kojima et al.; Chem. Pharm. Bull. 46(2), 373 (1998)