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S5540.0005
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Product information "5-Carboxyfluorescein diacetate N-succinimidyl ester / 5-FAM DA SE"
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside the cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein. Reagent for continuously determining the intracellular pH in bacteria by fluorometry. Labeling human intervertebral disk cells in vitro for fluorescence microscopy.
Specifications:
Assay: min. 95% (HPLC)
Identity determined by: NMR
Soluble in: DMSO and DMF
Fluorescence: λex 492 nm; λem 517 nm in 0.1M Tris pH8.0 (after cleavage by esterase)
C29H19NO11
MW = 557.46 g/mol
Source:
syntheticClassification:
CAS no.:
150206-05-6
eclass no.:
30-19-60-90
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Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.