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Customized Direct Genotyping PCR Kit

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Product information "Customized Direct Genotyping PCR Kit"

Customized Direct Genotyping PCR Kits – Tailored to Your Research Needs

Our customized Direct Genotyping PCR Kits offer rapid, real-time genotyping without the need for prior DNA isolation. By providing the specific SNP identifiers (rs-numbers) and species details (human, animal, or other), you can request a personalized kit designed to precisely match your requirements. Upon confirmation of your order, we will develop and deliver your customized genotyping kit within approximately 4-8 weeks.
Multiplex assays (2-4 targets simultaneously) can also be accommodated upon request. These multiplex reactions utilize fluorescence channels such as FAM, HEX, ROX, and Cy5 allowing simultaneous detection of multiple genetic markers. Please ensure your real-time PCR cycler is compatible with these fluorescence wavelengths and supports necessary color compensation functionalities.

Key features and benefits

  • No DNA Extraction Required: Perform direct PCR from crude samples like saliva, oral swabs, blood, or urine
  • Real-Time Genotyping: Efficient, rapid results without the delays associated with DNA isolation.
  • Hot-Start Technology: Allows reaction setup at room temperature, improving workflow convenience.
  • Universal Compatibility: Designed for compatibility with all common real-time PCR cyclers.
  • Reliable Fluorescence Detection: Accurate genotype determination using established fluorescence channels.
  • Eco-Friendly Shipping: Room-temperature stability eliminates the necessity for cold-chain logistics.

Ordering Information

  • Customized assays are priced as singleplex assays. Multiplex assays are available upon inquiry, with pricing determined individually.
  • Upfront payment is required for customized orders.

How the Technology Works

Real-time genotyping kits distinguish genetic variants based on melting curve analysis. Differences in melting peaks clearly identify homozygous wild-type, heterozygous, and homozygous mutant genotypes. Please note that minor shifts in melting temperatures may occur due to variations in sample composition (e.g., salt concentration or pH levels).
Contact us directly with your specific SNP (rs-number) and species details to start creating your customized Direct Genotyping PCR Kit.

More information? Watch our explainer video:

Application:

Direct Genotyping

Classification:

eclass no.: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


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