Direct HFE2 Genotyping PCR Kit
Advantages at a glance
- No DNA extraction required – Direct PCR from raw samples.
- Real-time genotyping – Faster and more efficient workflow.
- Hot-start formulation – Reaction setup at room temperature.
- Platform-independent – Compatible with all PCR cyclers.
- Reliable FAM fluorescence – Precise genotype determination.
- Eco-friendly shipping – Stable at room temperature.
€169.95
Product number:
G8040.0025
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Delivery time: 3-8 days
Shipment: not cooled. Store at -20°C. For laboratory usage only!
Product information "Direct HFE2 Genotyping PCR Kit"
rs1799945, HFE H63D Mutation - Fast, Real-Time Detection Without DNA Isolation
Designed for the precise identification of the HFE2 SNP C187G (rs1799945), this kit supports the detection of a genetic variant associated with hereditary hemochromatosis, particularly when present alongside other genetic variants that impact iron metabolism and storage. Early identification allows for proactive management to monitor iron levels and prevent complications related to iron overload.
The Genaxxon Direct HFE2 Genotyping PCR Kit enables real-time genotyping without the need for prior DNA isolation, allowing for a faster and more cost-effective workflow.
The Genaxxon Direct HFE2 Genotyping PCR Kit is the ideal solution for researchers and clinicians seeking a fast, sensitive, and reliable method for identifying hereditary hemochromatosis – all while saving time and costs.
Key Features & Benefits
- No DNA extraction required – Perform direct PCR from crude samples such as saliva, oral swabs, blood, and urine.
- Genotyping in real-time – Rapid and efficient workflow without time-consuming DNA isolation.
- Hot-start formulation – Enables convenient room-temperature reaction setup.
- Platform-independent – Compatible with all modern real-time PCR cyclers.
- Reliable fluorescence-based detection – FAM channel measurement ensures accurate genotype determination.
- Eco-friendly shipping – Stable at room temperature, reducing the need for cold-chain transport.
The Genaxxon Direct HFE2 Genotyping PCR Kit is the ideal solution for researchers and clinicians seeking a fast, sensitive, and reliable method for identifying hereditary hemochromatosis – all while saving time and costs.
How does it work?
Shown is exemplary genotyping data from two different blood samples.
The red melting curve originates from an EDTA blood sample and shows a single melting peak at 66°C. This clearly indicates the presence of a homozygous wild-type genotype.
In contrast, the green melting curve from another EDTA blood sample displays a pronounced melting peak at a significantly lower temperature (55°C). A single melting peak at this lower temperature indicates a homozygous mutant genotype. Conversely, the presence of two peaks in a melting curve (at 66°C and at 55°C) would be characteristic of a heterozygous genotype, thus indicating the presence of two different alleles in the sample.
Please note: minor shifts in melting temperatures can occur due to variations in salt concentration and pH in crude samples, which may influence the melting behavior slightly.
Picture 1:
More information? Watch our explainer video:
FAQs
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Quality is our top priority. Our raw material suppliers are regularly audited and maintain active quality management systems according to ISO 13485. Through internal quality controls, stability tests, controlled storage conditions, and guaranteed traceability, we ensure that we deliver only the highest quality products.
It’s possible that amplification curves are not visible, but this is not an issue. Please rely on the melting curves to determine the genotype of the respective sample. Always run postive and negative controls aside with each PCR experiment.
Yes, our kits are specifically designed for maximum compatibility. All our genotyping assays can run with the same PCR protocol.
Our kits are designed to be platform-independent and system-open. They are compatible with a variety of real-time PCR cyclers. Essentially, any cycler capable of detecting fluorescence in the FAM channel and performing a melting curve analysis will work for most assays. If you're unsure, feel free to contact our technical support (info@genaxxon.com).
Genotyping is determined by analyzing the melting curves. Analyze the melting peak temperature and the number of peaks. Two peaks indicate a heterozygous genotype, while a single peak suggests homozygosity. The exact genotype can be determined using the melting temperature. Please refer to the specified melting temperatures in the manual for each genotyping kit.
Our Genotyping Kits are designed for a variety of sample materials. The advantage of our kits is that you don't need to purify or extract the DNA – you can directly add the diluted sample to the PCR. This saves time and effort! If you have particularly valuable sample material, you can of course also use purified samples. Detailed instructions for compatible sample types can be found in our manuals at each product page.
Applikation / Application:
Direct GenotypingKlassifizierungen / Classification
eclass No.:
32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.