Advantages at a glance
- ready-to-use loading buffer
- 13 fragments from 250bp to 10,000bp
- extra bright bands at 1000bp and 3000bp as reference points
- very stable when stored at -20°C
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Product information "GenLadder 1kb (ready-to-use) DNA marker"
The GenLadder 1kb (ready-to-use) 250bp - 10kbp-ladder is the ideal DNA size marker for fragment sizes from 250 - 10000bp. All bands light up to the same intensity except the 1000 bp and the 3000 bp band which are twice as bright for better orientation. Each band has been calibrated with respect to base pair size and DNA quantity. Thus, an accurate determination of product size and DNA concentration is possible. No ambiguous bands are visible. The GenLadder 250bp - 10kb can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. The DNA is already dissolved in buffer with 6X loading dye ready to use.
Fragment sizes and amount of DNA per band:
10000bp ( 28ng/5µL), 8000bp (28ng/5µL), 6000bp (28ng/5µL), 5000bp (28ng/5µL), 4000bp ( 18ng/5µL), 2x3000bp (92ng/5µL), 2500bp (34ng/5µL), 2000bp (34ng/5µL), 1500bp (20ng/5µL), 2x1000bp (92ng/5µL), 750bp (23ng/5µL), 500bp (30ng/5µL), 250bp (45ng/5µL).
GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye.
The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
Specifications:
ready-to-use DNA marker containing already loading dye
Number of bands: 13
Fragment sizes and amount of DNA per band: 10000bp ( 28ng/5µL), 8000bp (28ng/5µL), 6000bp (28ng/5µL), 5000bp (28ng/5µL), 4000bp ( 18ng/5µL), 2x3000bp (92ng/5µL), 2500bp (34ng/5µL), 2000bp (34ng/5µL), 1500bp (20ng/5µL), 2x1000bp (92ng/5µL), 750bp (23ng/5µL), 500bp (30ng/5µL), 250bp (45ng/5µL).
DNA is dissolved at a concentration of 0.1µg/µL (500µL per 50µg)
Storage buffer: 10mM Tris-HCl, pH 7.5, 1mM EDTA, 10mM NaCl, 6 % Glycerol, 0.05 % Xylene cyanol, 0.05 % Bromophenol blue.
We recommend to load 5-6µL per lane.
Application:
Quantitative molecular weight standard for DNA electrophoresisSource:
syntheticClassification:
Documents:
Safety Data SheetProduct description
Accessory Items
High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of up to >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-S'. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer contains 15mM MgCl2. Free Taq DNA Polymerase test sample available!No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
For reliable and easy PCR: PCR Master Mix for standard PCR applications (e.g. screenings) for amplifying DNA fragments <5kb. It is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by routine PCR (one tube, one pipetting step). Just add your primers and template DNA - that´s all. The special formulation of our PCR Master Mix guarantees you reproducible results even after repeated freezing/thawing steps leading to good yields and sensitivity. The PCR Master Mix is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for 24 months. Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.
High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-E'. The buffer is optimised for high yield amplification of DNA-templates. Our complete buffer contains 25mM MgCl2. Taq DNA Polymerase test sample available! No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
RedMasterMix (2x) - PCR MasterMix with red dye to help visualize pipetting and mixing steps. Electrophoresis can be performed immediately after PCR without the need of a gel loading buffer. This makes this MasterMix time efficient, cost efficient and a reliable choice for the best PCR results with high efficiency. One tube, one pipetting step. RedMasterMix (2x) is delivered to you as a optimized, ready-to-use solution containing- Taq DNA Polymerase - dNTPs- MgCl2- a red dye- reaction bufferfor efficient amplification of DNA. Additionally the RedMasterMix (2x) contains an additive and a red dye for proceeding with electrophoresis after PCR without adding loading buffer. Just add your primers and template DNA. This PCR MasterMix (2x) has been optimized for use in routine PCR amplification of DNA templates in the range of 0.2-4kb. The special formulation of our PCR RedMasterMix (2x) can withstand repeated freezing/thawing without compromising yields, sensitivity or results, when used as directed. The PCR MasterMix with red dye is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for at least 24 months.Our RedMastermix can also be used for Sanger sequencing. Just dilute the PCR reaction 1:8 after PCR, or purify using spin columns and then apply. Read now in our blog how Genaxxon's RedMasterMix can also simplify your laboratory work. Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.
Accessory Items
For reliable and easy PCR: PCR Master Mix for standard PCR applications (e.g. screenings) for amplifying DNA fragments <5kb. It is a premixed, ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by routine PCR (one tube, one pipetting step). Just add your primers and template DNA - that´s all. The special formulation of our PCR Master Mix guarantees you reproducible results even after repeated freezing/thawing steps leading to good yields and sensitivity. The PCR Master Mix is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for 24 months. Test sample available at a special price! The test sample price will be refunded on the first official order of the product. Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.
The GenLadder 50bp (ready-to-use) is an ideal DNA size marker for fragment sizes from 50 - 1500bp. The bands at 200bp, 500bp and 1200bp bands light up more intensive for better orientation. Each band has been calibrated with respect to base pair size. Thus, an accurate determination of product size is possible. No ambiguous bands are visible. Band sizes: 50 (ca. 30ng/5µL), 100 (ca. 27ng/5µL), 150 (ca. 30ng/5µL), 200 (2x) (ca. 67ng/5µL), 250 (ca. 25ng/5µL), 300 (ca. 20ng/5µL), 350 (ca. 18ng/5µL), 400 (ca. 27ng/5µL), 450 (ca. 23ng/5µL), 500 (2x) (ca. 67ng/5µL), 600 (ca. 20ng/5µL), 700 (ca. 23ng/5µL), 800 (ca. 27ng/5µL), 900 (ca. 30ng/5µL), 1000 (ca. 33ng/5µL), 1200 (2X) (ca. 70ng/5µL) and 1500bp (ca. 40ng/5µL). GenLadder 50bp is already dissolved in loading buffer with tracking dye (Orange G) that does not overlab with bands in the range between 500bp and and smaller. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
RedMasterMix (2x) - PCR MasterMix with red dye to help visualize pipetting and mixing steps. Electrophoresis can be performed immediately after PCR without the need of a gel loading buffer. This makes this MasterMix time efficient, cost efficient and a reliable choice for the best PCR results with high efficiency. One tube, one pipetting step. RedMasterMix (2x) is delivered to you as a optimized, ready-to-use solution containing- Taq DNA Polymerase - dNTPs- MgCl2- a red dye- reaction bufferfor efficient amplification of DNA. Additionally the RedMasterMix (2x) contains an additive and a red dye for proceeding with electrophoresis after PCR without adding loading buffer. Just add your primers and template DNA. This PCR MasterMix (2x) has been optimized for use in routine PCR amplification of DNA templates in the range of 0.2-4kb. The special formulation of our PCR RedMasterMix (2x) can withstand repeated freezing/thawing without compromising yields, sensitivity or results, when used as directed. The PCR MasterMix with red dye is efficient (no left over of reagents), scalable from 10µL to 50µL and stable for at least 24 months.Our RedMastermix can also be used for Sanger sequencing. Just dilute the PCR reaction 1:8 after PCR, or purify using spin columns and then apply. Read now in our blog how Genaxxon's RedMasterMix can also simplify your laboratory work. Our Agaroses > and DNA Ladders > are ideally suited for subsequent electrophoresis of PCR products.
Our DNA marker GenLadder 100 bp Plus with loading dye is an ideal tool to determine the size of double stranded DNA in the range of 100 - 3000 bp. The additional bands at 1.5kbp and 3.0kbp allow to assign additional DNA fragment sizes in the range up to 3000 bp without using an additional 1kbp DNA marker. The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/5µL), 2x 1500 bp (70ng/5µL), 1000 bp (50ng/5µL), 900 bp (40ng/5µL), 800 bp (40ng/5µL), 700 bp (30ng/5µL), 600 bp (30ng/5µL), 2x 500 bp (90ng/5µL), 400 bp (40ng/5µL), 300 bp (30ng/5µL), 200 bp (40ng/5µL), 100 bp (40ng/5µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 0.1µg/µL (108µg/mL). The recommended amount of DNA marker per lane is 0,5μg (5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye detectable with blue light (ca. 490nm excitation). Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0,5μg (6µL).Please note: The included dye does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation. We therefore recommend using blue light to achieve the best possible results. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.