Advantages at a glance
- Activity: min. 20,000 U/mg (pH6.2)
- Appearance: white freeze-dried powder, albumin-free
- DNAse and RNAse free.
- MW: approx. 14,400 g/mol
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Product information "Lysozyme from chicken egg white, min. 20000 U/mg - Molbio Grade"
Lysozyme (Muramidase) preferentially hydrolyses the β-1,4-glycosidic binding between N-Acetyl muraminic acid and N-Acetyl glucosamine, a component of the proteoglycan-cell wall of certain microorganisms. The enzyme is present in many organisms. In molecular biology, the enzyme from chicken white egg is used to lyse E. coli for the isolation of plasmid-DNA. The working concentration is 200-300 μg/µL. To increase the plasmid yield (approx. 5-10%) lysozyme may be added. Another application is the lysis of bacteria for the preparation of bacterial RNA. In this case the working concentration is 40μg/mL (stock solution 50mg/mL).
Stability: The lyophilized powder of lysozyme is stable for many years at +2°C to +8°C. In solution, the stability at pH values from 4 to 5 at +2°C to +8°C is several weeks and at room temperature several days. pH-optimum is at 9.2, the isoelectric point at 11.0.
Lysozyme will be inhibited by surfactants like SDS or alcohols and fatty acids, imidazole and indol-derivatives.
Specifications:
Activity: min. 20,000 U/mg (pH6.2)
Appearance: white freeze-dried powder, albumin-free
DNAse and RNAse free.
MW: approx. 14,400 g/mol
Stability (lyophilised and stored at -20°C): several years / (in solution at +2°C to +8°C): several weeks.
Application:
Plasmid isolation RNA isolation protoplasts generationSource:
from chicken egg whiteClassification:
Documents:
CertificateProduct description
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Accessory Items
Genaxxon´s Total RNA Purification Kit - Tissue is designed for the rapid and efficient purification of high quality RNA from 1-30mg of tissue (fresh or frozen) or up to 25mg Paraffin embedded tissue. The isolation protocol and buffer formulations were optimised for high isolation efficiency and purity of RNA. The Total RNA Purification Kit utilises spin columns with membranes which efficiently and selectively bind nucleic acids at high concentration of chaotropic salts. The isolation procedure consists of 6 steps. In the first isolation step, the tissue is homogenized in order to disintegrate intercellular bonds (epithelial tissue) and fragmentize high-molecular proteins (muscle or connective tissue). Then the homogenate is lysed. Any RNases are inactivated. The three-step washing stage effectively removes impurities and enzyme inhibitors. The purified RNA is eluted using a low ionic strength buffer or RNase-free water and can be used directly in all downstream applications such as RT-PCR, Northern blotting, RT-qPCR and so forth. Genaxxon offers also ceramic beads > for rapid cell disintegration and other RNA purification kits >. With our HotScriptase RT Polymerase >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step. Here you will find further Purification kits >.
Accessory Items
The enzyme Lysozyme (Muramidase) affects the cell walls of bacteria. Thereby the extraction efficiency of proteins or nucleic acids is significantly improved. Genaxxon Lysozyme from chicken egg is available as lyophilized powder. Lysozyme preferentially hydrolyses the β-1,4-glycosidic binding between N-Acetyl muraminic acid and N-Acetyl glucosamine, a component of the proteoglycan-cell wall of certain microorganisms. The enzyme is present in many organisms. In molecular biology, the enzyme from chicken white egg is used to lyse E. coli for the isolation of plasmid-DNA. The lysis of E.coli is improved by the combined addition of both lysozyme and and a nuclease such as DNase I (M3028) >. The working concentration is 200-300 μg/µL. To increase the plasmid yield (approx. 5-10%), lysozyme may be added. Another application is the lysis of bacteria for the preparation of bacterial RNA. In this case the working concentration is 40μg/mL (stock solution 50mg/mL). Lysozyme from chicken egg is most effective for the lysis of gram-positive bacteria. Besides this, it facilitates the lysis of gram-negative bacteria such as Salmonella and Shigella. Lyophilized, white powder Enzyme for lysis of bacterial cell walls Improvement of protein purification from inclusion bodies Activity: min. 20.000 U/mg (pH: 6.25) Lysozyme will be inhibited by surfactants like SDS (M3292) > or alcohols and fatty acids, Imidazole (M6033) > and Indol-derivatives. Stability: The lyophilized powder of lysozyme is stable for many years at +2°C to +8°C. In solution, the stability at pH values from 4 to 5 at +2°C to +8°C is several weeks and at room temperature several days. pH-optimum is at 9.2, the isoelectric point at 11.0.