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M3183.0050
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Product information "Dextran sulfate 500 Sodium salt"
Dextran sulfate is a polyanion produced by esterification of dextran with chlorosulphonic acid. Dextran sulfate 500 is a dextran with an average molecular weight of approx. 500,000 Daltons.
Anionic dextrans such as dextran sulfate (10% in hybridisation buffer) accelerate the hybridisation of nucleic acids by a factor of approx. 10.
Synonyms: 6-O-(6-O-beta-D-Glucopyranosyl-beta-D-glucopyranosyl)-D-glucose, Dextran 500, Manninotriose
Dextran sulfate is routinely used for
- Selective precipitation of lipoproteins
- Probe hybridization of membrane-immobilized DNA
- Release of DNA from DNA-histone complexes
- Inhibition of RNA binding to ribosomes
Specifications
Average MW: appr. 500.000 g/mol
Solubility: (10%; H2O): clear slightly yellow
pH (1%; H2O): 5.0 - 7.5
Sulfated ash: 40 - 50%
Loss on drying: 10%
Chloride: max. 0.1%
Average MW: appr. 500.000 g/mol
Solubility: (10%; H2O): clear slightly yellow
pH (1%; H2O): 5.0 - 7.5
Sulfated ash: 40 - 50%
Loss on drying: 10%
Chloride: max. 0.1%
Application:
Advanced hybridisation of nucleic acids - Selective precipitation of lipoproteins - Probe hybridization membrane-immobilized DNASource:
syntheticClassification:
EC no:
232-677-5
CAS no.:
9011-18-1
eclass no.:
31-43-02-90
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Documents:
Safety Data SheetCertificate