Advantages at a glance
- Low molecular weight DNA
- DNAse and RNAse activities: not detected
- Protein free
- Very low bioburden
- MW: <2500 Da (main component: approx. 2000 bp)
| Quantity | Unit price |
|---|---|
| To 3 |
€127.39
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| To 6 |
€114.65
€127.39
(10% saved)
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| From 7 |
€101.91
€127.39
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Product information "Salmon Sperm DNA (Na salt)"
DNA extracted from salmon testes. To prevent unspecific binding of a DNA to the membrane in Southern- and Northern-Hybridisations salmon sperm DNA is added to the pre-hybridisation solution (Blotto, Denhardt's etc.). This Salmon Sperm DNA shows a low molecular weight.
The lyophilised DNA can be dissolved in sterile bidistilled water (stock solution e. g. 10mg/mL) and stored at -20°C. This DNA is used with a final concentration of 100μg/mL (Ref.: Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY (1989), 9.48-9.49, 13.15. Silhavy, T., et al., Experiments with Gene Fusions, Cold Spring Harbor, NY (1984), 195.)
Specifications:
Low molecular weight DNA
DNAse and RNAse activities: not detected
protein free
very low bioburden
MW: <2500 Da (main component: approx. 2000 bp)
lyophilised powder
Application:
To prevent unspecific binding of the probe to the membrane in Southern- and Northern-Hybridisations salmon sperm DNA is added to the pre-hybridisation solution (Blotto, Denhardt's etc.). The lyophilised DNA ist dissolved in sterile bidistilled water (stock solution e. g. 10mg/mL) and stored at -20°C. This DNA is used with a final concentration of 100μg/mL.Source:
Salmon spermClassification:
Documents:
CertificateAccessory Items
Spermine, a polyamine, is derived from spermidine and is required for cell growth in eukaryotes. It acts as regulator of gene expression, inhibits DNA damage and protects the cells from damage by acting as free radical scavenger. In proteomics studies, spermine mediates rapid crystallization of DNA-binding proteins. Spermine is a natural occuring polyamin, which is found in human sperm and other body fluids with a characteristic odour. Spermine is a polyamine involved in cellular metabolism found in all eukaryotic cells. The precursor for synthesis of spermine is the amino acid ornithine. It is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at physiological pH. Spermine is associated with nucleic acids and is thought to stabilize helical structure, particularly in viruses. Additionally it is used as starting material for the synthesis of medical products. (Source: Wikipedia: https://en.wikipedia.org/wiki/Spermine).