Uracil-DNA Glycosylase (UDG)

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€89.20

Delivery time 1–2 days

Units

200 units

1000 units

Product number: M3096.0200

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Product information "Uracil-DNA Glycosylase (UDG)"

Uracil DNA Glycosylase (UDG), also called uracil N-glycosylase (UNG), catalyzes the release of uracil from uracil-containing DNA. The UDG/UNG from Genaxxon is a highly purified, recombinant enzyme isolated from E. coli with a mass of 26 kDa. UDG efficiently hydrolyzes single- and double-stranded DNA containing uracil, except for oligomers with 6 or fewer bases. Therefore, uracil-DNA glycosylase (UDG) is used wherever it is necessary to avoid the risk of DNA carryover (contamination) during PCR.

In order to be able to hydrolyze DNA by UDG/UNG you do need dUTP > in your PCR reactions.

Storage buffer:

20mM Tris
HCl (pH 8.0)
50mM NaCl
0.1mM EDTA
50% Glycerin

Reaction buffer (1X):

20 mM Tris
HCl (pH 8.0)
1 mM EDTA

FAQs

In general, the polymerase stops replication at the abasic site. However, because the polymerase would always start replication if there is a primer binding site with an annealed primer, there is a possibility of getting some kind of "amplification". If the primer binding site contains a T, the UDG would cut the uracil and thus damage the primer binding site. If the primer binding site is still intact (because it does not contain a T), the polymerase could indeed replicate the DNA strand to the next abasic site. However, because there is no complementary strand, the amplification would not be exponential but more or less linear.

Specifications:
Activity: 10 units/µL.

delivered in storage buffer: 20mM Tris, HCl (pH 8.0), 50mM NaCl, 0.1mM EDTA, 50% glycerol

Application:

for prevention of carry over of DNA in PCR

Units:

One unit of activity is defined as the amount of enzyme required to release 60 pmol of uracil per minute from double-stranded, uracil containing DNA.

Source:

E.coli

Classification:

eclass no.: 32-16-04-10

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Safety Data Sheet
Protocols
Certificate
Manuals

Accessory Items

dUTP solution 100mM

Deoxyuridine 5'-triphosphate, tetrasodium salt (dUTP) of purity >99% (HPLC). dUTP may be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. Product has been tested for PCR products of length up to 8kb (Taq DNA polymerase) and 0.8kb (Pfu DNA polymerase). The substitution of dTTP for dUTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme Uracil-DNA-Glycosylase, UDG >, can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Genaxxon offers also PCR dNTP-Mix 10 mM >, unser dNTP-Set as 4 x 100 mM solution > or modified nucleotides as Biotin-11-dUTP >. For your successful PCR you will find different enzymes or PCR master mixes: Taq DNA Polymerse (M3001) >, Proof-Reading Polymerases Pfu (M3004) >, Pwo (M3002) > and ReproFast (M3003) >, as well as our ready-to-use RedMastermix (M3029) >, containing dNTPs already.

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