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GNX Food Probe qPCR MasterMix 5X

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Product information "GNX Food Probe qPCR MasterMix 5X"

Reliable qPCR for Food, Environmental and Inhibitor-Rich Samples

The GNX Food Probe qPCR MasterMix (5X) is specifically designed for fast, reliable and highly sensitive probe-based qPCR from food samples, environmental specimens and other challenging biological materials. Engineered for exceptional inhibitor tolerance, this master mix delivers robust amplification performance even when working with difficult sample matrices containing PCR inhibitors.

The included modified HotStart Taq DNA Polymerase provides outstanding specificity and sensitivity while minimizing non-specific amplification. This ensures reliable and reproducible results.

A key feature of the system is the combination of the 5X Master Mix with the separately added GNX Food Probe Booster (10X). This dedicated booster further enhances resistance against common PCR inhibitors, enabling consistent amplification performance under demanding assay conditions.

The master mix is also lyo-ready, making it an excellent choice for the development of stable, room-temperature assay formats. In addition, the incorporation of dUTP allows effective carry-over contamination control when used together with UDG (Uracil-DNA Glycosylase). (Please note: UDG is not included!)

Since the HotStart DNA polymerase remains inactive at room temperature, reaction setup can be performed without cooling, simplifying workflow and reducing the risk of non-specific amplification. The optimized buffer system supports rapid reaction kinetics and efficient target amplification across a broad range of applications.

Key Benefits

  • Specifically optimized for food, environmental and inhibitor-rich samples
  • Exceptional inhibitor tolerance for reliable qPCR performance
  • High sensitivity and specificity
  • Innovative two-component system with GNX Food Probe Booster (10X)
  • Convenient room-temperature reaction setup
  • Fast HotStart technology without activation step
  • Contains dUTP for optional UDG-based contamination control
  • Lyo-ready for lyophilized assay development

Applications

  • Food pathogen detection
  • Food safety testing and quality control
  • Environmental monitoring
  • Veterinary diagnostics
  • Water testing
  • Detection of microbial contamination
  • qPCR from inhibitor-rich sample matrices

Further realtime PCR Mastermixes and realtime FAST PCR Mastermixes can be found here: qPCR (Classic qPCR > or FAST and Multiplex qPCR >).

This ProbeMastermix is a ready-to-use 5-time master mix that contains no ROX™.

Aliquot size: 400µL. This master mix is stable for at least 8 months at +2°C to +8°C.

Application:

qPCR from food and diary derived samples

Source:

synthetic

Classification:

eclass no.: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

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