Advantages at a glance
- Binding capacity: up to 40µg DNA. Gel slices with a weight of up to 300mg can be used.
- Elution volume: >50µL
- Fragment size: 100bp to 10kbp
- Type of DNA recovered: ssDNA and dsDNA
- Yields: PCR: up to 99%, Gel extraction: up to 95%
- Time required:
PCR: 5-10 minutes
Gel extraction: 16-20 minutes
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Product information "PCR and Gel Extraktion Mini Prep Kit"
The Genaxxon PCR and Gel Extraction Mini Prep Kit is a 3-in-1 kit for fast and efficient purification of DNA from PCR, enzymatic reactions but also for DNA extraction from standard agarose gels or low melting point agarose gels. The kit combines the advantages of spin column technology with the selective binding properties of a silica membrane, eliminating the need for toxic phenol-chloroform extractions.
The PCR and Gel Extraction Mini Prep Kit effectively removes primers, primer dimers, dNTPs, unincorporated labelled nucleotides, enzymes and salts from PCR reaction mixtures. The kit is optimised for the purification of DNA fragments from 50 bp to 10 kb but can also be used for smaller or larger fragments.
The recovery rates are 95% for the size range from 100 bp to 3 kb DNA fragments. Smaller (50bp) or larger fragments (20kbp) can be isolated with a reduced yield (>70%).
Applications
The DNA fragments purified with this PCR and gel extraction kit can be used directly for all applications, including- Sequencing
- Microarray analysis
- Ligation and transformation
- Restriction digestion
- Labelling
- Microinjection
- PCR and in vitro transcription.
The kit includes
- Binding Buffer- Prewash Buffer
- Wash Buffer
- Elution Buffer
- DNA Purification Micro Columns & Collection Tubes
You can buy separate PCR DNA purification columns (TS5313 >) which can be used for Genaxxon´s kits or for these of competitors!
Binding capacity: approx. 40µg DNA
Gel slices with a weight of up to 300mg can be used
Yields:
PCR: up to 99%, depending on DNA fragment length (in the range of 100 bp – 10 kb)
Gel extraction: up to 95%, depending on DNA fragment length (in the range of 100 bp – 10 kb)
Purity: A260/A280 ratio: 1.7 – 1.9
Time required:
PCR: 5-10 minutes
Gel extraction: 16-20 minutes
Application:
DNA purification from enzymatic reactions (PCR, restriction digest, etc.); DNA purification from DNA gels.Classification:
Documents:
ManualsSource: NCBI PubMed
Anna Burrichter, Karin Denger, Paolo Franchini, Thomas Huhn, Nicolai Müller, Dieter Spiteller, David Schleheck
Front Microbiol. 2018 Nov 27;9:2792.
doi: 10.3389/fmicb.2018.02792.
PMCID: PMC6278857.