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Product information "Empty Spin Columns 20 mL for Affinity Chromatography"
Empty Spin Columns for standard protein purification procedures, e.g. affinity chromatography with Ni-IDA, Ni-NTA, Co-IDA oder Co-NTA agaroses. The smallest column size does have a capacity of 0.5mL (S) and are ideal for protein optimisation process screenings. Our L columns (20mL) are a very cost effecitve option if you like to order IDA- or NTA-agarose in bulk and fill columnes by yourself.
Our empty spin columns are offered in the sizes: 0.5mL (S) >, and 20mL (L) >. Easy Handling. Useable under native and denaturing conditions.
For being able to use our spin columns you need a centrifuge with fixed angle (45° for our 500µL columns), or with a swing bucket rotor (for the 20mL columns).
Specifications:
Maximal volume: 20mL
Max. g-force: 1,500 x g (swing-bucket rotor)
Columns made from Polypropylene
Filter pore size: 0.1-0.2µm
Membrane: low binding PVDF. Maximal volume: 20mL. Max. g-force: 1,500-2,000 x g (swing-bucket rotor).
Typical spin times: 5-10 min at 400 x g.
Application:
standard protein purification affinity protein purificationClassification:
Documents:
ProtocolsManuals
General Data 1