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Product information "Ni-IDA MagBeads for His-tagged protein purification"
Protein purification based on magnetic beads has become popular because they are useful to extract proteins from diluted solutions, such as cell culture supernatants purify proteins expressed at low levels perform pull-down experiments We use magnetic beads with a ferrimagnetic core and an agarose coating coupled to a ligand of choice, our beads meet our own high quality standards. They enable fast and easy purification steps, which can be automated. The amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Genaxxon MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel or cobalt ions. All offered ligand-metal systems efficiently bind histidine-tagged proteins. With a binding capacity of up to 40mg protein per mL (Ni-IDA) and 70mg per mL (Ni-NTA) of settled MagBeads, Genaxxon Ni-IDA MagBeads are comparable to equivalent beads from alternative providers.
Application:
Affinity chromatography, Purification of recombinant Proteins carrying a polyhistidine tagSource:
syntheticClassification:
Documents:
Safety Data SheetProtocols
Manuals
General Data 1
General Data 2
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Accessory Items
Nickel-loaded agaroses are the most frequently used products for the efficient purification of 6×histidine-tagged recombinant fusion proteins. Because Ni2+ ions show high affinity for 6×histidine-tagged fusion proteins, extremely high yields result from purification of these fusion proteins from the lysates. However, if purification takes place from lysates containing foreign protein chains with repeated histidine elements, selectivity with respect to 6×histidine-tagged fusion protein decreases as non-tagged histidine-containing proteins increasingly also bind to the Ni-IDA agarose. In this case the use of Genaxxon Co-IDA Agarose is recommend (cat#: S5370), which features far higher selectivity with respect to 6×histidine-tagged fusion protein. Genaxxon Ni-IDA Agarose has high chemically and pH stability and enables purification directly from raw lysates. Genaxxon Ni-IDA Agarose can be regenerated several times with nickel sulfate solution for re-use. Features of Ni-IDA-Agarose - a highly efficient and favorable way for the separation of 6-His tagged fusion proteins. Highlights: - ideal for grafity-flow and batch procedures - no expensive purification system necessary - consistent performance - for native and denaturing conditions. The Genaxxon Ni-IDA Agarose consists of cross-linked Agarose, covalently bound to Iminodiacetic acid loaded with Ni2+-ions. Binding capacity: up to 50mg 6xHis tagged fusion protein per mL Agarose. Application: Affinity chromatography of 6-His tagged fusion proteins in batch or grafity flow procedure. Ni-IDA Agarose is supplied as a 50% buffered suspension with 20% Ethanol.