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Product information "Pre-Packed Ni-IDA Agarose columns"
Genaxxon bioscience offers pre-packed Ni2+ IDA columns in ready-to-use format for His-tag protein purification processes by gravity flow. Fast purifications with good yield of target proteins are obtainable by this method. Both matrices Ni2+- and Co2+-IDA-Agarose and 2 different bed volumes (1mL, 5mL) are available supplied as suspension in 20% ethanol. Frit Pore Size 20µm - 6% crosslinked agarose (Sepharose®CL-6B) -Matrix Stability in all commonly used reagents for protein purifications - Activating Agent Epichlorohydrin -Bed Volume Agarose 1mL (for L-Column), 5mL (for XL-Column) - Autoclavable at 121°, 30 min. - Capacity ca. 28 µmol Ni2+/mL gel, ca. 25 µmol Co2+/mL gel.
Specifications:
6% cross-linked Agarose (Sepharose(R)CL-6B)
Chemical stability: stable in all commonly used reagents for protein purification
Autoclavable at 121°C for 30 min..
Classification:
Documents:
Safety Data SheetProtocols
Manuals
General Data 2
Accessory Items
Nickel-loaded agaroses are the most frequently used products for the efficient purification of 6×histidine-tagged recombinant fusion proteins. Because Ni2+ ions show high affinity for 6×histidine-tagged fusion proteins, extremely high yields result from purification of these fusion proteins from the lysates. However, if purification takes place from lysates containing foreign protein chains with repeated histidine elements, selectivity with respect to 6×histidine-tagged fusion protein decreases as non-tagged histidine-containing proteins increasingly also bind to the Ni-IDA agarose. In this case the use of Genaxxon Co-IDA Agarose is recommend (cat#: S5370), which features far higher selectivity with respect to 6×histidine-tagged fusion protein. Genaxxon Ni-IDA Agarose has high chemically and pH stability and enables purification directly from raw lysates. Genaxxon Ni-IDA Agarose can be regenerated several times with nickel sulfate solution for re-use. Features of Ni-IDA-Agarose - a highly efficient and favorable way for the separation of 6-His tagged fusion proteins. Highlights: - ideal for grafity-flow and batch procedures - no expensive purification system necessary - consistent performance - for native and denaturing conditions. The Genaxxon Ni-IDA Agarose consists of cross-linked Agarose, covalently bound to Iminodiacetic acid loaded with Ni2+-ions. Binding capacity: up to 50mg 6xHis tagged fusion protein per mL Agarose. Application: Affinity chromatography of 6-His tagged fusion proteins in batch or grafity flow procedure. Ni-IDA Agarose is supplied as a 50% buffered suspension with 20% Ethanol.