Advantages at a glance
- DNA extraction & Hot-Start PCR in one kit
- PCR-ready DNA in just 8 minutes
- No centrifugation required
- Enhanced specificity with SuperHot Mastermix
- Direct gel loading with built-in blue loading dye
Product number:
M3261.0100
Our benefits
- Free shipping
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For precise information regarding the delivery date, please contact Genaxxon.
Shipment: on wet ice. Stored at -20°C. For laboratory usage only!
Product information "DNA Q-Extraction Hot-Start PCR Kit"
Fast DNA Extraction & Hot-Start PCR Kit
The DNA Q-Extraction Hot-Start PCR Kit combines our rapid DNA Q-Extraction Solution with the high-performance SuperHot Mastermix (2X) Blue. This all-in-one kit enables fast DNA extraction and reliable Hot-Start PCR – with PCR-ready DNA in just 8 minutes, without any additional purification steps.
Ideal for genotyping and the amplification of DNA from a wide range of sources including mammalian tissues, bacterial cells, saliva, and oral swabs.
Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.
Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.
Key Features
- Combined DNA extraction & hot-start PCR
- Fast 8-minute protocol
- No centrifugation needed
- PCR-ready DNA from cells and tissues
- Includes loading dye for direct gel loading and pipetting control
Application
The simple one-step lysis is performed in two heating steps (65 °C / 98 °C) using a thermocycler or heating block. The resulting lysate contains PCR-ready DNA, suitable for standard and real-time PCR, without vortexing, or centrifugation.
The included SuperHot Mastermix (2X) Blue contains a hot-start Taq polymerase for improved specificity and sensitivity. The integrated blue loading dye allows direct gel loading and visual control of pipetting.
Kit Components
Extracted DNA is stable at -20 °C for up to one week, and for long-term storage at -80 °C.
Also available as a non-hot-start version with our reliable Red Mastermix (2X) – perfect for robust results in routine PCR.
Application Notes:
application note genotyping with m3231_dna_q_extraction_solution_en_vs250527
Specifications:
Kit consisting of:2-time ready-to-use master mix for Hotstart PCR with chemically inactivated Taq polymerase, dNTPs, buffer
and a blue dye for better visualization of pipetting and as gel loading dye.
Convenient 1.25mL aliquots.
DNase/RNase free lysis buffer for isolation of nucleic acids from different tissue/samples.
non-toxic solution
Application:
nucleic acid extraction for subsequent hot-start PCRUnits:
SuperHot Mastermix: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.Source:
syntheticClassification:
eclass no.:
32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.