Advantages at a glance
- DNA extraction and PCR in one convenient kit
- PCR-ready DNA in just 8 minutes
- No centrifugation, dilution, or vortexing needed
- Direct PCR from cells, tissues, or saliva
- Includes loading dye for direct gel loading
Product number:
M3251.0100
Our benefits
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
For precise information regarding the delivery date, please contact Genaxxon.
Shipment: on wet ice. Stored at -20°C. For laboratory usage only!
Product information "DNA Q-Extraction PCR Kit Red"
Fast DNA Extraction & PCR in One Kit
The DNA Q-Extraction PCR Kit Red combines the powerful DNA Q-Extraction Solution with our reliable Red Mastermix (2X) – enabling rapid and easy DNA extraction followed by PCR amplification in a single streamlined workflow.
Perfect for genotyping, this kit delivers PCR-ready DNA in just 8 minutes from a variety of sample types such as mammalian cells, bacteria, saliva, buccal swabs, or tissue samples.
Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps.
This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.
Your Benefits at a Glance
- Fast 8-minute protocol
- No vortexing, centrifugation or dilution
- PCR-ready DNA directly from lysate
- Simple protocol: lysis and PCR with minimal handling
- Non-toxic reagents – safe and convenient
- Scalable and automation-friendly
- Direct gel loading thanks to built-in loading dye
Application
The lysis is performed in two simple heating steps (65 °C for 6 min, 98 °C for 2 min) using a thermocycler or heating block. The resulting DNA is immediately ready for PCR without any further purification – ideal for genotyping applications.
The included Red Mastermix (2x) ensures reliable amplification results and enables direct loading onto agarose gels with visual pipetting control.
Kit Components
Extracted DNA is stable at -20 °C for up to one week. For long-term storage, we recommend -80 °C.
Also available as a hot-start version with our SuperHot Mastermix (2X) Blue – ideal for increased specificity with complex templates.
Application Notes:
application note genotyping with m3231_dna_q_extraction_solution_en_vs250527
Specifications:
Kit consisting of:
2-time ready-to-use master mix for PCR including a red dye for better visualization of pipetting and as gel loading dye. dNTPs, buffer and DNA polymerase already included.
Convenient Aliquot size: 1.25mL. This master mix is stable for at least 8 months at +2°C to +8°C.
DNase/RNase free lysis buffer for isolation of nucleic acids from different tissue/samples.
non-toxic solution
Application:
nucleic acid extraction for subsequent PCRUnits:
Red Mastermix: One unit is defined as the amount of enzyme which will convert 10 nmoles of dNTPs to an acid-insoluble form in 30 min at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-amino-propanesulfonic acid, sodium salt) pH 9.3 (25°C); 50 mM KCl; 2 mM MgCl2; 1 mM b-mercaptoethanol; and activated calf thymus DNA as substrate.Source:
syntheticClassification:
eclass no.:
32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.