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1.4mm Beads of G2 DNA/RNA Extraction Enhancer

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Product information "1.4mm Beads of G2 DNA/RNA Extraction Enhancer"

G2 DNA/RNA Enhancer for improved DNA/RNA extraction!
The G2 DNA/RNA Enhancer is developed to increase the yield of microbial DNA during DNA extraction from difficult matrices for example clay and soil samples. The primary function of G2 DNA/RNA Enhancer is to relieve inhibitory DNA - particle or other inhibitory DNA complexes.

FEATURES
- Inhibition of DNA and RNA sorption to clay particles
- At least 2-10 fold increased yield of microbial DNA and RNA.
- No trace of endonuclease-, nicking-, exonuclease- or RNase activity
- Patent EP2 443 251

DESCRIPTION
G2 DNA/RNA Enhancer is available freeze dried with 0.1mm or 1.4mm ceramic beads in a 2mL tube. G2 DNA/Enhancer is also available in liquid format in three packing sizes including either 10, 50 or 100 reactions of 500µL.

G2 DNA/RNA Enhancer should be used in combination with either a standardised extraction methods or commercial purification kits intended for DNA & RNA extraction.

G2 DNA/RNA ENHANCER INCREASES DNA YIELD FROM SOIL SIGNIFICANTLY.

G2 DNA/RNA Enhancer is available with 0.1mm ceramic beads (> A4201xx) or with 1.4mm ceramic beads (> A4214xx) and as solution (> A4200xx).

Protocol for using G2 DNA/RNA Enhancer Beads

How to use G2 DNA/RNA Enhancer in combination with commerical extraction kits:

This protocol serves as a guideline for extraction of DNA and RNA from difficult matrices, such as soil clays, soils, activated coal etc. when using the G2 DNA/RNA Enhancer (G2) in combination with a commercial DNA or RNA extraction kit intended for soil.

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FAQs
Yes, the G2 DNA/RNA Enhancer can be used either in combination with a standard extraction method or in combination with an extraction kit.
The G2 DNA/RNA enhancer reagent is based on eukaryotic DNA and consists of short fragments of modified double-stranded DNA. (Jacobsen et al. (2018), Scientific Reports 8:5711). By adding the blocking reagent G2 to the DNA/RNA isolates, the binding sites of the soil colloids for the nucleic acids are occupied. The DNA/RNA normally bound to soil colloids is released and is available for DNA/RNA isolation.
No, the G2 DNA/RNA enhancer has no impact on subsequent molecular analyses.

Specifications:
Ceramic beads for increase DNA/RNA yield of clay, soil and wine samples.
Ceramic beads of 0.1mm diameter.

Usable only together with commercially available DNA/RNA purification kits.

Application:

DNA and RNA purification of soil or clay samples

Source:

synthetic

Classification:

eclass no.: 32-17-02-90
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Product description
Spec. Product Description
General Data 1
General Data 2
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed

1. Inter-laboratory testing of the effect of DNA blocking reagent G2 on DNA extraction from lowbiomass clay samples.
SCIENTIFIC RepoRtS | (2018) 8:5711 | DOI:10.1038/s41598-018-24082-y

2. Quantitative and qualitative evaluation of the impact of the G2 enhancer, bead sizes and lysing tubes on the bacterial community composition during DNA extraction from recalcitrant soil core samples based on community sequencing and qPCR.
PLOS ONE | https://doi.org/10.1371/journal.pone.0200979 April 11, 2019

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G2 DNA and RNA extraction enhancer solution by Genaxxon

G2 DNA/RNA Enhancer for improved DNA/RNA extraction!The G2 DNA/RNA Enhancer is developed to increase the yield of microbial DNA during DNA extraction from difficult matrices for example clay and soil samples. The primary function of G2 DNA/RNA Enhancer is to relieve inhibitory DNA - particle or other inhibitory DNA complexes. FEATURES- Inhibition of DNA and RNA sorption to clay particles- At least 2-10 fold increased yield of microbial DNA and RNA.- No trace of endonuclease-, nicking-, exonuclease- or RNase activity- Patent EP2 443 251 DESCRIPTIONG2 DNA/RNA Enhancer is available freeze dried with 0.1mm or 1.4mm ceramic beads in a 2mL tube. G2 DNA/Enhancer is also available in liquid format in three packing sizes including either 10, 50 or 100 reactions of 500µL. G2 DNA/RNA Enhancer should be used in combination with either a standardised extraction methods or commercial purification kits intended for DNA & RNA extraction. G2 DNA/RNA ENHANCER INCREASES DNA YIELD FROM SOIL SIGNIFICANTLY. G2 DNA/RNA Enhancer is available with 0.1mm ceramic beads (> A4201xx) or with 1.4mm ceramic beads (> A4214xx) and as solution (> A4200xx).Protocol for using G2 DNA/RNA Enhancer BeadsHow to use G2 DNA/RNA Enhancer in combination with commerical extraction kits:This protocol serves as a guideline for extraction of DNA and RNA from difficult matrices, such as soil clays, soils, activated coal etc. when using the G2 DNA/RNA Enhancer (G2) in combination with a commercial DNA or RNA extraction kit intended for soil.

Regular price: From €132.61