Advantages at a glance
- Room-temperature reaction setup – no cooling required during pipetting
- Warm-start feature of RTase – prevents nonspecific cDNA synthesis before reaction start
- Extended stability – full performance maintained after 7 days at 37 °C in premixed all-in-one reactions
- Ultra-fast cDNA synthesis – possible in just 2 minutes
- High sensitivity – reliable detection even with low-copy RNA templates
- Convenient handling – premixed single-tube setup simplifies workflows and reduces errors
Product number:
M3068.1010
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Product information "Challenger Hotstart Reverse Transcriptase"
Why Choose Challenger Hotstart RT?
- Ultra-Fast cDNA synthesis – Achievable in just 2 minutes.
- Extended stability – In pre-assembled all-in-one reaction mixtures, Challenger Hotstart RT maintains full performance even after 7 days at 37 °C.
- Room-temperature setup – Pipette without cooling, saving time and reducing errors.
- Warm-start reverse transcriptase – Activated only at high temperatures, preventing nonspecific cDNA synthesis before the reaction begins.
- Exceptional thermostability – Over 99 % of enzyme activity remains sealed at 42 °C for controlled and reliable activation.
- Versatile applications – Ideal for rapid cDNA synthesis, RT-PCR, and RT-qPCR with challenging RNA templates
Optimized for High-Performance Reverse Transcription Workflows
Challenger Hotstart RT is designed for demanding applications. Its Warm-Start technology keeps the enzyme inactive during setup, enabling precise control over the start of cDNA synthesis. The improved thermostability ensures robust performance: Challenger Hotstart RT remains inactive at 42 °C and is only activated at higher temperatures. Therefore, a standard reverse transcription reaction with oligo(dT) primers or random hexamer primers at 42 °C is not possible with this enzyme.
Fast and Reliable cDNA Synthesis
Challenger Hotstart RT allows efficient reverse transcription even with templates that have complex secondary structures or high GC content. Its enhanced thermostability ensures rapid and accurate cDNA generation without compromising specificity or yield.
Long-Term Stability and Easy Handling
Engineered for routine and high-throughput lab work, Challenger Hotstart RT supports room-temperature setup, simplifying workflows and reducing errors. Its robust formulation guarantees reliable storage and shipping stability.
Typical Applications
- cDNA synthesis from total RNA or mRNA, including difficult templates.
- One-step or two-step RT-qPCR with high sensitivity, even at low RNA input.
- Research and diagnostic workflows requiring reproducible results.
- High-throughput applications, including multiplexed panels or screening assays.
Key Features Summary
- Ultra-Fast cDNA synthesis – as quick as 2 minutes.
- Extended stability – retains full performance after 7 days at 37 °C in premixed reaction setups.
- Warm-start reverse transcriptase – delayed activation improves specificity.
- High thermostability – supports challenging RNA templates.
- Room-temperature setup – simplifies workflow, reduces errors.
- Robust performance – suitable for routine and high-throughput applications.
FAQs
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The Challenger Hotstart RT is a genetically engineered reverse transcriptase (RT) that transcribes RNA into cDNA. Its key feature is a "hot-start" property, which is achieved through a specific aptamer-inhibitor.
The hot-start mechanism prevents enzyme activity at room temperature. This significantly reduces the formation of non-specific products, leading to higher specificity and yield in cDNA synthesis. The enzyme only becomes fully active at temperatures above 55°C.
The Challenger HotStart RT is excellently suited for cDNA synthesis. Due to its hot-start property, which only allows activity at temperatures above 55°C, it is ideally used with gene-specific primers whose annealing temperature lies in this high range.
No, the Challenger Hotstart RT is not designed for use with oligo(dT) or random hexamer primers. The reason is a temperature incompatibility: these primer types typically require reaction temperatures below 42°C to bind efficiently. Since the Challenger Hotstart RT only becomes active above 55°C, an efficient cDNA synthesis with these primers is not possible.
Product Specifications
Concentration: 200 units/µL
Nuclease contamination: No
Protease contamination: No
E-coli DNA: <1 copy
Units:
One unit incorporates 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as template-primer.Classification:
eclass no.:
32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.