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Product information "Bio Lp-1 qPCR Kit for purification and detection of Legionella ssp., L. pneumophila serogroups"
The Bio Lp-1 assay is designed to target specific and unique DNA sequences within the Legionella genome. The PCR products from 4 different reactions can be detected by specific DNA probes labeled with 4 different fluorophores. This allows the simultaneous detection of all the Legionella species associated with infection, all the L. pneumophila serogroups (1-16) and specifically identifies L. pneumophila sg-1, responsible for ~95% human infections. An internal amplification control is also included in the master mix as an indicator that the PCR is functioning correctly.
Designed to work on the leading real-time PCR platforms, Bio Lp-1 rapidly detects extremely low levels of Legionella, and can differentiate between Legionella spp, all L. pneumophila serogroups 1-16 and L. pneumophila-serogroup 1simultaneously in a single water sample, giving a complete and accurate Legionella profile. With this certainty, the exact risk to public health can be quickly determined and acted upon. Particularly beneficial in cases of an outbreak, appropriate remedial actions can be taken, their efficacy monitored to reduce and remove Legionella bacteria from a contaminated water system. In less than 5 hours, water quality and safety can be assured. Simple and convenient to use, and in a freeze-dried format, the test contains all necessary components to process a water sample directly.
Bio Lp-1-Legionella multiplex one step qPCR Kit is a diagnostic breakthrough, being the world’s first open platform rapid NAD kit for simultaneously detecting and identifying multiple species of Legionella from water in a single test.
The lyophilized master mix is aliquoted into 12 x 8 low profile PCR Tube strips packed in an aluminium foil bag together with a DNA purification kit and with negative and positive DNA control enabling to prepare standards of 10E5 GU, 10E4 GU, 10E3 GU, 10E2 GU, 10E1 GU (with 1 GU = one genomic unit) and a DNA purification kit that is optimized for the isolation of Legionella DNA isolated from water samples.
| Description |
Bio Lp-1 Legionella Quantification Kit |
| Sample type | Water |
| Cycler compatibility | Roche Diagnostics LightCycler 480 system II, Bio-Rad CFX96 Touch, Thermo QuantStudio |
| Basis for detection | Probe based |
| Format | Lyophilized powder (PCR assay, Positive control DNA, PCR standards), Liquid (Negative control) |
| No. of reactions |
96 |
| Components | qPCR assay - 12 x 8 well qPCR strips (foil wrapped) |
| Positive control DNA - lyophilised | |
| Negative control - liquid | |
| Legionella DNA for quantification - lyophilised | |
| Bio Lp-1 DNA Isolation Kit | |
| PCR method | qPCR multiplex |
| Storage | Room temperature. Positive control and DNA for quantification, +2°C to +8°C. -20°C after rehydration. |
| PCR efficiency |
88.12 - 99.46% |
|
Quantification limit |
15 GU |
|
Detection limit |
5 GU |
| Use | Water testing |
Application:
water test on legionellaClassification:
Documents:
ProtocolsManuals
List of bacteria that have been tested to be not detected with our Legionella Multiplex PCR kit. These bacteria will be part of the ISO 12869 certification.
Aeromonas hydrophila
Alcaligenes faecalis (e.g. CIP 60.80)
Bacillus subtilis (e.g. CCM 1999)
Burkholderia cepacia
Clostridium spp.
Enterobacter aerogenes
Escherichia coli (e.g. WDCM 00003)
Flavobacterium spp.
Klebsiella oxytoca (e.g. WDCM 00179)
Listeria monocytogenes (e.g. WDCM 00109 or WDCM 00020)
Proteus vulgaris
Pseudomonas aeruginosa
Pseudomonas fluorescens
Pseudomonas putida
Serratia marcescens
Stenotrophomonas maltophila.
These bacteria have been tested as part of the ongoing certification process and all have come up negative when using the Bio Lp-1 kit for detection of Legionella.
Source: NCBI PubMed
Legionella References
- Legionella (Legionnaires' Disease and Pontiac Fever) Treatment and Complications. Centers for Disease Control and Prevention (CDC). 26 January 2016. Archived from the original on 29 March 2016. Retrieved 21 March 2016.
- Verhasselt et al. (2021) COVID-19 Co-infection with Legionella pneumophila in 2 Tertiary-Care Hospitals, Germany. Emerging Infectious Diseases, Vol27, No5
- Ashbolt, N. J. (2021) Legionella Infection during and after the COVID-19 Pandemic, ACS EST Water, Vol1, pp13-14
- Palazzolo, C. et al. (2020) Legionella pneumonia: increased risk after COVID-19 lockdown? Italy, May to June 2020, Eurosurveillance, Vol25, No30
- Legionella Control, https://legionellacontrol.com/international/us-covid-shutdown-legionnaires-outbreaks/ (accessed on 20 April 2021)
- ...
Accessory Items
Sterile distilled water free of DNAses, Rnases and Proteinases. Water treated with DEPC to ensure absence of Rnase. For that reason this water is especially suited for RT-PCR or other molecular biology application where 100% absence of RNAse is necessary (resuspension of DNA, dilution of enzymes, etc.). Genaxxon offers different grades of nuclease free water: sterile, Diethylpyrocarbonate (DEPC) treated water (M6082 >) and nuclease free water (not DEPC treated) (M6340 >).
Deoxynucleotide (dNTP) Solution Mix as an equimolar solution of ultrapure, HPLC purified (>99%) dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. The Genaxxon dNTP solutions are optimized for use in DNA amplification and other related methods. The Genaxxon dNTPs and dNTP-mixes contain no measurable bacterial or human DNA. For long term storage and/or for repeated use we do recommend to aliquot the stock solutions. Mixture of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 10 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix withv2 mM dNTP mix , our dNTP set, or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
Pure, quality tested, nuclease-free water is suitable for use in all experiments that require nuclease-free water, including molecular biology applications. Nuclease-free water is processed, to yield DNase, RNase, and nuclease-free, deionized water. Contamination of water with nucleases may lead to inconsitencies or even complete failures of experiments. For this Genaxxon offers different grades of nuclease free water: sterile, Diethylpyrocarbonate (DEPC) treated water (M6082 >) and nuclease free water (not DEPC treated) (M6340 >). Our Nuclease-Free Water is provided in nuclease-free PE bottles or in glass bottles.
Highly pure, HPLC purified (>99%) dNTPs packaged as 4 separate 100mM solutions of dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. Genaxxon’s dNTP solutions have been optimized for use in DNA amplification and other related methods. Genaxxon dNTPs contain no measurable bacterial or human DNA. For long term storage and/or for repeated use, our recommendation is to aliquot the stock solutions. Solutions of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 100 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mixes with 2 mM or 10 mM or the modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003) as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
Highly pure, HPLC purified dNTPs (>99%) delivered as a 2 mM solution of dATP, dCTP, dGTP and dTTP for use in qPCR, standard PCR, RT-PCR and Klenow reactions. Use 5 µL of Mix for PCR in 50µL reaction volume.The Genaxxon dNTP mix is optimized for its use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Mixture of dNTP sodium salt solutions (dATP, dCTP, dGTP und dTTP) Concentration: 2 mM of ach nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix with 10 mM dNTP mix, our dNTP set , or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
Accessory Items
The Legionella Detection Multiplex qPCR Assay is designed to target specific and unique DNA sequences within the Legionella genome. The PCR products from 4 different reactions can be detected by specific DNA probes labeled with 4 different fluorophores. This allows the simultaneous detection of all the Legionella species associated with infection, all the L. pneumophila serogroups (1-16) and specifically identifies L. pneumophila sg-1, responsible for ~95% human infections. An internal amplification control is also included in the master mix as an indicator that the PCR is functioning correctly.Designed to work on the leading real-time PCR platforms, Bio Lp-1 rapidly detects extremely low levels of Legionella, and can differentiate between Legionella spp, all L. pneumophila serogroups 1-16 and L. pneumophila-serogroup 1 simultaneously in a single water sample, giving a complete and accurate Legionella profile. With this certainty, the exact risk to public health can be quickly determined and acted upon. Particularly beneficial in cases of an outbreak, appropriate remedial actions can be taken, their efficacy monitored to reduce and remove Legionella bacteria from a contaminated water system. In less than 5 hours, water quality and safety can be assured. Simple and convenient to use, and in a freeze-dried format, the test contains all necessary components to process a water sample directly. Bio Lp-1-Legionella multiplex one step qPCR Kit is a diagnostic breakthrough, being the world’s first open platform rapid NAD kit for simultaneously detecting and identifying multiple species of Legionella from water in a single test. The kit comes together with negative and positive DNA control enabling to prepare standards of 10E5 GU, 10E4 GU, 10E3 GU, 10E2 GU, 10E1 GU (with 1 GU = one genomic unit). TACT US > TO GET A TEST SAMPLE LEARN MORE > GET MORE INFORMATION