Advantages at a glance
- Ultra-pure dNTPs (>99%), HPLC-purified
- Optimized for qPCR, standard PCR, RT-PCR, and Klenow reactions
- No detectable bacterial or human DNA
- Mix with 10 mM concentration per nucleotide for precise applications
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Product information "dNTP Mix (Na salt) - 10mM"
Deoxynucleotide (dNTP) Solution Mix as an equimolar solution of ultrapure, HPLC purified (>99%) dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. The Genaxxon dNTP solutions are optimized for use in DNA amplification and other related methods.
The Genaxxon dNTPs and dNTP-mixes contain no measurable bacterial or human DNA. For long term storage and/or for repeated use we do recommend to aliquot the stock solutions.
- Mixture of dNTP sodium salts (dATP, dCTP, dGTP und dTTP)
- Concentration: 10 mM each nucleotide
Please have also a look on our broad range of nucleotides especially the dNTP mix withv2 mM dNTP mix , our dNTP set, or modified nucleotides, e.g. Biotin-11-dUTP.
You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
Specifications:
10 mmol/L mix of each dNTP (dATP, dCTP, dGTP, dTTP).
Sodium salt of each dNTP
Purity of each dNTP: min. 99% (HPLC)
No DNases, RNases, proteases detectable. No human or E.coli DNA detectable.
1mL corresponds to 10 µmol of each dNTP.
dATP: λmax 259nm; e 15.4 λ mmol-1 cm-1 (Tris/HCl, pH7.0)
dCTP: λmax 271nm; e 8.9 λ mmol-1 cm-1 (Tris/HCl, pH7.0)
dGTP: λmax 252nm; e 13.7 λ mmol-1 cm-1 (Tris/HCl, pH7.0)
dTTP: λmax 262nm; e 9.6 λ mmol-1 cm-1 (Tris/HCl, pH7.0)
Application:
PCR qPCR Multiplex PCRSource:
syntheticClassification:
Documents:
Safety Data SheetCertificate
Product description
General Data 1
Source: NCBI PubMed
Chip-based duplex real-time PCR for water quality monitoring concerning Legionella pneumophila and Legionella spp.
Cornelia Reuter, Stefanie Hentschel, Antje Breitenstein, Eileen Heinrich, Oliver Aehlig, Thomas Henkel, Andrea Csáki, Wolfgang Fritzsche
Water Environ. J., 35: 371-380.
doi: 10.1111/wej.12635
Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces
Christian Weisenstein, Dominik Schaar, Anna Katharina Wigger, Heiko Schäfer-Eberwein, Anja K. Bosserhoff, Peter Haring Bolívar
Biomed Opt Express. 2020 Jan 1; 11(1): 448–460.
doi: 10.1364/BOE.380818
PMCID: PMC6968760
Monitoring the threatened utility of malaria rapid diagnostic tests by novel high-throughput detection of Plasmodium falciparum hrp2 and hrp3 deletions: A cross-sectional, diagnostic accuracy study
Andrea Kreidenweiss, Franziska Trauner, Miriam Rodi, Erik Koehne, Jana Held, Lea Wyndorps, Gédéon Prince Manouana, Matthew McCall, Ayola Akim Adegnika, Albert Lalremruata, Peter G. Kremsner, Rolf Fendel, Thaisa Lucas Sandri
EBioMedicine. 2019 Dec; 50: 14–22.
doi: 10.1016/j.ebiom.2019.10.048
PMCID: PMC6921222
Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces.
Christian Weisenstein, Dominik Schaar, Anna Katharina Wigge, Heiko Schäfer-Eberwein, Anja K Bosserhoff, Peter Haring Bolívar
Biomed Opt Express. 2019 Dec 23;11(1):448-460.
doi: 10.1364/BOE.380818.
PMCID: PMC6968760.
The circadian clock is functional in eosinophils and mast cells
Anja Baumann, Simone Gönnenwein, Stephan C Bischoff, Hadas Sherman, Nava Chapnik, Oren Froy, Axel Lorentz
Immunology. 2013 Dec; 140(4): 465–474.
doi: 10.1111/imm.12157
PMCID: PMC3839650
Low Density Lipoprotein Receptor-related Protein 1 (LRP1) Modulates N-Methyl-d-aspartate (NMDA) Receptor-dependent Intracellular Signaling and NMDA-induced Regulation of Postsynaptic Protein Complexes
Chikako Nakajima, Akos Kulik, Michael Frotscher, Joachim Herz, Michael Schäfer, Hans H. Bock, Petra May
J Biol Chem. 2013 Jul 26; 288(30): 21909–21923.
doi: 10.1074/jbc.M112.444364
PMCID: PMC3724646
Reelin induces EphB activation
Elisabeth Bouché, Mario I Romero-Ortega, Mark Henkemeyer, Timothy Catchpole, Jost Leemhuis, Michael Frotscher, Petra May, Joachim Herz, Hans H Bock
Cell Res. 2013 Apr; 23(4): 473–490.
doi: 10.1038/cr.2013.7
PMCID: PMC3616423
γ-Secretase Mediates Self-Limitation of the Inflammatory Response by Processing LRP1
Kai Zurhove, Chikako Nakajima, Joachim Herz, Hans H. Bock, Petra May
Sci Signal. 2008 Nov 25; 1(47): ra15.
doi: 10.1126/scisignal.1164263
PMCID: PMC2694618
Comparative phylogenomic analyses of teleost fish Hox gene clusters: lessons from the cichlid fish Astatotilapia burtoni
Simone Hoegg, Jeffrey L Boore, Jennifer V Kuehl, Axel Meyer
BMC Genomics. 2007; 8: 317.
doi: 10.1186/1471-2164-8-317
PMCID: PMC2080641
Accessory Items
High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of up to >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-S'. The buffer is optimised for high specificity amplification of DNA-templates. Our complete buffer contains 15mM MgCl2. Free Taq DNA Polymerase test sample available!No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
Agarose LE is a standard agarose for the separation of DNA in the size range between 100bp and 25kbp. It is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 100bp and 25kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc. This agarose is comparable with, e.g. Agarose BioRagent, low EEO from Sigma or with the Universal-Agarose, peqGOLD from Peqlab. Free Taq DNA Polymerase test sample available! No shipping costs within Germany. Genaxxon offers Agaroses for different purposes. Two melting point options and normal or high resolution separation of DNA fragments are available. Standard normal melting agarose M3044 with standard resolution used in routine DNA electrophoresis for separation of a wide range of DNA fragments from 100bp up to 25kbp. This agarose is available as powder (M3044 Agarose LE >) or as high-quality, multi-purpose tablets (M3054 >) available in blister packaging in 200 or 1,000 tablet quantities. They are compact, pre-weight and therefore easy-to-use. Low melting/gelling temperature agarose recommended for rapid DNA gel extraction with the agarose-digesting enzyme, agarase, as well as for "in-gel" DNA treatment with enzymes or for bacterial transformation with nucleic acids directly after re-melting the gel. High resolution agarose (normal melting or low melting) for high resolution elektrophoresis to differentiate DNA fragments with only 2 bp size difference: M3046 Agarose Tiny > (low melt, high resolution comparable to NuSieve®), or M3047 Agarose Tiny HT > (high gel strength, high resolution comparable to SeaKem®). For the nontoxic staining of nucleic acids in agarose gels the highly sensitive fluorescent dye SafeGel red stain (M3193) or SafeGel green stain (M3191) is recommended.
High-quality Taq DNA Polymerase from Genaxxon is a highly processive 5' - 3' DNA Polymerase, lacking 3' - 5' exonuclease activity. The high processivity and fidelity of Genaxxon bioscience Taq Polymerase allows amplification of DNA fragment of >7 kb. Genaxxon bioscience Taq Polymerase is delivered with 10X reaction buffer and separate MgCL2.The enzyme is delivered with our buffer component 'Buffer-E'. The buffer is optimised for high yield amplification of DNA-templates. Our complete buffer contains 25mM MgCl2. Taq DNA Polymerase test sample available! No shipping costs within Germany. With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
Accessory Items
Highly pure, HPLC purified dNTPs (>99%) delivered as a 2 mM solution of dATP, dCTP, dGTP and dTTP for use in qPCR, standard PCR, RT-PCR and Klenow reactions. Use 5 µL of Mix for PCR in 50µL reaction volume.The Genaxxon dNTP mix is optimized for its use in DNA polymerisation and related methods. Our dNTPs contain no measurable bacterial or human DNA. For storage for a prolonged periode of time we recommend to prepare small aliquots, especially in case of rare use. Mixture of dNTP sodium salt solutions (dATP, dCTP, dGTP und dTTP) Concentration: 2 mM of ach nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mix with 10 mM dNTP mix, our dNTP set , or modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003), as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
Highly pure, HPLC purified (>99%) dNTPs packaged as 4 separate 100mM solutions of dATP, dCTP, dGTP and dTTP for qPCR, RT-PCR, standard PCR, and Klenow reactions. Genaxxon’s dNTP solutions have been optimized for use in DNA amplification and other related methods. Genaxxon dNTPs contain no measurable bacterial or human DNA. For long term storage and/or for repeated use, our recommendation is to aliquot the stock solutions. Solutions of dNTP sodium salts (dATP, dCTP, dGTP und dTTP) Concentration: 100 mM each nucleotide Please have also a look on our broad range of nucleotides especially the dNTP mixes with 2 mM or 10 mM or the modified nucleotides, e.g. Biotin-11-dUTP. You can get additional products for your successful PCR as our Taq DNA Polymerse (M3001), or our proof-reading polymerases Pfu (M3004), Pwo (M3002) and ReproFast (M3003) as well as the ready-to-use RedMastermix (M3029), already including dNTPs.
2',3'-Dideoxyadenosine-5'-O-triphosphate (ddATP) sodium salt of purity >95% HPLC. For other salt forms or a guaranteed higher purity please inquire. 2',3'-Dideoxyadenosine-5'-Triphosphate (ddATP) is a modified nucleoside triphosphate, where the 2' and 3' hydroxyl groups are absent, resulting in chain termination while PCR. The inability of polymerases to extend from a dideoxy nucleotide causes the chain termination and is widely useful in antiviral research and in a variety of biotechnology applications. ddATP is used in cycle sequencing, enzyme mechanistic studies and for producing RNA and DNA sequences that cannot be extended by polymerases or joined by DNA ligases. Another notable application that utilizes a primer terminated on the 3′ end with a dideoxy modification is pyrophosphorolysis-activated polymerization (PAP). This technique is valuable for the detection of rare mutations. Reference: Sanger et al., Proc. Natl. Acad. Sci. USA, 74, 5463 - 5467 (1977). ddATP is an analogue of cyditine triphosphate (ATP) where both ribose hydroxy groups have been removed and replaced by hydrogen.