Advantages at a glance
- ready-to-use Formel: enthält nicht nur Ladepuffer, sondern auch Nukleinsäurefärbemittel
- Nicht-toxisches und nicht-mutagenes Gel-Färbemittel
- 13 Fragmente in Größen zwischen 250 - 10.000bp
- Innovation für Hochdurchsatz oder Colony screenings
- Free shipping
- Shipping within 24h
- 30 days money back guarantee
- Purchase comfortable on account
Product information "GenLadder 1 kbp Plus with gel stainining dye"
The GenLadder 1kb bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 250 and 10,000 base pairs.
At the same time, the mixture also contains a fluorescent gel staining dye detectable with blue light (ca. 490nm excitation). Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step.
This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens!
The Gene Ladder 1kbp Plus consists of 13 fragments in sizes between 250 - 10,000bp.
The 1000bp and 3000bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography.
Fragment sizes (in base pairs): 10.000 bp (28ng/6µL), 8000 bp (28ng/6µL), 6000 bp (28ng/6µL), 5000 bp (28ng/6µL), 4000 bp(18ng/6µL), 2x 3000 bp (92ng/6µL), 2500 bp (34ng/6µL), 2000 bp (34ng/6µL), 1500 bp (20ng/6µL), 2x 1000 bp (92ng/6µL), 750 bp (23ng/6µL), 500 bp (30ng/6µL), 250 bp (45ng/6µL).
The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL.
The recommended amount of DNA marker per lane is 0.5-0.6μg (5-6µL).
Please note: The included dye does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation. We therefore recommend using blue light to achieve the best possible results.
The GenLadder with gel staining dye is also available as a GenLadder 100bp Plus (M3237).
GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye.
The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
Specifications:
ready-to-use DNA marker containing already loading dye plus a fluorescence gel staining dye for visualization of DNA bands
Number of bands: 13
Fragment sizes: 250 bp, 500 bp, 750 bp, 2x1000 bp, 1500 bp, 2000 bp, 2500 bp, 2x 3000 bp, 4000 bp, 5000 bp, 6000 bp, 8000 bp and 10,000 bp.
DNA is dissolved at a concentration of 90µg per 1mL.
Buffer contains orange G & xylene cyanol FF as tracking dyes.
Buffer contains a fluorescent gel staining dye for visualization of DNA bands.
We recommend to load 5-6µL per lane.
Application:
Molecular weight standard for DNA electrophoresisSource:
syntheticClassification:
Documents:
ManualsAccessory Items
Ready-to-use PCR Mastermix with red Loading Dye for visual control of the pipetting steps and additional fluorescent dye for fast and easy detection of the DNA bands. After PCR, the PCR mix can be pipetted directly into the gel pockets without adding loading buffer. This makes our RedMasterMix Fluoro (2x) even more time- and cost-saving than our proven Red Mastermix. The RedMasterMix Fluoro (2x) with fluorescent gel staining dye and red loading dye also features high specificity for best results. The RedMasterMix Fluoro (2x) is a ready-to-use mixture of: - Taq DNA Polymerase- PCR reaction buffer- dNTPs- MgCl2- red loading dye- fluorescence gel staining dye for DNA band detection in an optimal concentration for efficient amplification of DNA templates by PCR. Only the primers and the template DNA have to be added. At the same time, the PCR Mastermix contains an additive and a red dye, which allows subsequent electrophoresis without the addition of loading buffer. After electrophoresis, detection is performed directly under blue light (ca. 490nm) without further staining. This saves additional time and costs. The RedMasterMix Fluoro (2x) with fluorescent dye was developed for use in routine PCR up to 4 kb amplicon length. The special composition of the buffer guarantees reproducible results even after repeated thawing and freezing cycles. Our Red MasterMix (2x) Fluoro is shipped in convenient aliquots of 1.25mL.Please note: The included fluorescent dye does show only a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation at 490nm. We therefore recommend using blue light to achieve the best possible results.
Accessory Items
The GenLadder 100 bp Plus with gel staining dye and loading dye - ready-to-use is an innovation for high throughput approaches or colony screenings! Ideally suited to determine the size of double-stranded DNA between 100 and 1000 base pairs. The additional bands at 1.5kbp and 3.0kbp allow DNA sizes in this range to be assigned without having to use an additional 1kbp DNA marker. At the same time, the mixture also contains a fluorescent gel staining dye detectable with blue light (ca. 490nm excitation). Additional staining (pre- or post staining) or the addition of fluorescent dye prior to application is thus unnecessary and saves an additional pipetting step. This DNA ladder is an ideal match for our Red MasterMix Fluoro 2X, which also contains the gel staining dye. Together the ideal pair for Colony Screens! The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/6µL), 2x 1500 bp (70ng/6µL), 1000 bp (50ng/6µL), 900 bp (40ng/6µL), 800 bp (40ng/6µL), 700 bp (30ng/6µL), 600 bp (30ng/6µL), 2x 500 bp (90ng/6µL), 400 bp (40ng/6µL), 300 bp (30ng/6µL), 200 bp (40ng/6µL), 100 bp (40ng/6µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 90 µg/mL. The recommended amount of DNA marker per lane is 0,5μg (6µL).Please note: The included dye does show a small excitation peak in the UV range, but fluorescence is significantly stronger under blue light excitation. We therefore recommend using blue light to achieve the best possible results. GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3084 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
The GenLadder 1kb (ready-to-use) 250bp - 10kbp-ladder is the ideal DNA size marker for fragment sizes from 250 - 10000bp. All bands light up to the same intensity except the 1000 bp and the 3000 bp band which are twice as bright for better orientation. Each band has been calibrated with respect to base pair size and DNA quantity. Thus, an accurate determination of product size and DNA concentration is possible. No ambiguous bands are visible. The GenLadder 250bp - 10kb can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. The DNA is already dissolved in buffer with 6X loading dye ready to use. Fragment sizes and amount of DNA per band: 10000bp ( 28ng/5µL), 8000bp (28ng/5µL), 6000bp (28ng/5µL), 5000bp (28ng/5µL), 4000bp ( 18ng/5µL), 2x3000bp (92ng/5µL), 2500bp (34ng/5µL), 2000bp (34ng/5µL), 1500bp (20ng/5µL), 2x1000bp (92ng/5µL), 750bp (23ng/5µL), 500bp (30ng/5µL), 250bp (45ng/5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.
Our DNA marker GenLadder 100 bp Plus with loading dye is an ideal tool to determine the size of double stranded DNA in the range of 100 - 3000 bp. The additional bands at 1.5kbp and 3.0kbp allow to assign additional DNA fragment sizes in the range up to 3000 bp without using an additional 1kbp DNA marker. The Gene Ladder 100 bp Plus consists of 12 fragments in sizes between 100 - 1000bp spaced 100bp apart. In addition, there are further bands at 1500bp and 3000bp. The 500bp and 1500bp fragment show stronger intensity to allow easier identification. All fragments are 'blunt-ended'. The GenLadder can be directly labeled at the 5'-end with radioisotopes using T4 polynucleotide Kinase for visualisation by autoradiography. Fragment sizes (in base pairs): 3000 bp (40ng/5µL), 2x 1500 bp (70ng/5µL), 1000 bp (50ng/5µL), 900 bp (40ng/5µL), 800 bp (40ng/5µL), 700 bp (30ng/5µL), 600 bp (30ng/5µL), 2x 500 bp (90ng/5µL), 400 bp (40ng/5µL), 300 bp (30ng/5µL), 200 bp (40ng/5µL), 100 bp (40ng/5µL). The DNA marker is already dissolved in buffer containing orange G and Xylene cyanol FF as tracking dyes. The total concentration of the DNA-Marker is 0.1µg/µL (108µg/mL). The recommended amount of DNA marker per lane is 0,5μg (5µL). GenLadder DNA ladders are available in a ready-to-use format (premixed with 6X DNA Loading Dye): M3094 (GenLadder 1kbp extended up to 25 kbp ready-to-use) > and M3328 (GenLadder 1kbp ready-to-use) > or our ready-to-use premix M3072 (GenLadder 50bp) > with 6X Orange DNA Loading Dye. The DNA Gel Loading Dyes can also be ordered separately: M3308 (DNA Gel Loading Dye with Bromophenol and Xylene Cyanol FF) >, M3321 (DNA Gel Loading Dye with Orange G) >.