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Product information "E-64 cystein protease inhibitor"
E-64 is an irreversible inhibitor of different cysteine proteases (papain, bromelain, ficin, cathepsin B, H and L, tumor-cathepsin, calpain, V-CATH from Autographa californica M nucleopolyhedro virus (AcMNPV) and Streptococcus-protease). All enzymes are completely inhibited by a concentration of 10 - 30 μM within 30 minutes.
E-64 and other protease inhibitors (e. g. diisopropylfluorophosphate and PMSF) are able to inhibit the programmed cell death, that is induced by activation of the T cell receptor in different cell lines of the immune system, not by blocking the signal transduction. The effect of dexamethason, which induces apoptosis as an antagonist of the T cell receptor, is enhanced.
If proteins are expressed in the baculo virus system with a functional v-cath gene, very often a proteolytic activity can be observed. This reduces the quality of the expressed protein significantly. The addition of E-64 to all reaction steps avoids the degradation almost completely.
Stability:
E-64 is stable against reducing agents. Stock solutions have concentrations between 1mM and 10mM in DMSO or 50% ethanol and are stable for several days, if stored at +4°C. Aliquots may be stored at -20°C for approx. one month.
Synonyme: trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butan; L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatin; N-(trans-Epoxysuccinyl)-L-leucin-4-guanidinobutylamid
You will find further protease inhibitors here: Inhibitors >: Pepstatin A >, AEBSF >, Leupeptin >, TLCK >, PMSF >, Bestatine > or Diisopropylphosphorofluoridat (DFP) >.
Specifications:
Assay: min. 98.0%
Appearance: white, crystaline powder
Solubility (2% in water): clear, coulorless solution
C15H27N5O5
MW = 357.40 g/mol
Classification:
Documents:
General Data 1Category List
Accessory Items
Papain is a member of the class of proteolytic enzymes that needs a free sulfhydryl group for activity (group of the cysteine proteases). The pH optimum of Papain is approx. pH 6 and the optimum temperature for activity is 65°C. At the isoelectric point of approx. pH 9.6, the best solubility is achieved. Please note that even at this point, Papain will be dissolved incompletely. The recommended solvent is water.