Advantages at a glance
- Purity: >98% (HPLC)
- Appearance: White solid crystals
- Solubility (10% in water): Clear, colourless solution
- Loss on Drying: ≤ 3%
- Conductivity: ≤10μS/cm (0.1M in water)
- MW: 614.89 g/mol
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Product information "CHAPS for 2D-gel electrophoresis"
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150 Da) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).
Specifications:
Purity: >98% (HPLC)
Appearance: White solid crystals
Solubility (10% in water): Clear, colourless solution
Loss on Drying: ≤ 3%
Conductivity: ≤10μS/cm (0.1M in water)
C32H58N2O7S
MW = 614.89 g/mol
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CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).