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Product information "CHAPS buffer grade"
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).
Specifications:
Purity: >98% (HPLC)
Appearance: White solid crystals
Solubility (10% in water): Clear, colourless solution
Loss on Drying: ≤ 3%
Conductivity: ≤10μS/cm (0.1M in water)
C32H58N2O7S
MW = 614.89 g/mol
Source:
syntheticSafety Information:
Classification:
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CHES shows a pKa (25°C) of 9,55 which makes CHES useful as a buffering component in the pH range between 8.6 to 12.0. CHES interferes in the protein quantification according to Lowry. It is used in crystallisation of Phosphotriesterase (50mM) or in the chemical modification of Bacteriorhodopsin (10mM). Synonyms are: 2-(Cyclohexylamino)ethanesulfonic acid; 2-(N-Cyclohexylamino)ethanesulfonic acid. Other buffers are: BES, BICIN, MOPS, CAPS, CHAPS
MOPS is used as a buffer substance at pH 6.5 to 7.9. (Goods N.E., Izawa S. (1972) Methods Enzymol., 24, 53, Ferguson W.K. et al. (1980) Anal. Biochem., 104, 300).
![CHAPSO; 3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate](https://www.genaxxon.com/media/e0/8a/9b/1708287096/m6202_chapso.jpg?ts=1708287096 "m6202_chapso")
3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulphonate (CHAPSO) is a zwitterionic detergent and is used as a non-denaturing buffering agent. CHAPSO has similar properties to CHAPS, but possesses an additional hydroxyl group (-OH), making it more polar and more soluble. CHAPSO is frequently used for the solubilisation of membrane proteins.
Buffering substance in the pH range 6,1-7,5. Min. 99.5% pure. Good N.E. et al. (1966) Biochemistry 5, 467-77, Shipman jr. Ch. (1969) Proc. Soc. Exp. Biol. Med. 130, 305-28.
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry, isoelectric focusing and two-dimensional electrophoresis. Useful for solubilizing membrane proteins and breaking protein-protein interactions. Its small micellar molecular weight (6150 Da) and high critical micellar concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilisation for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Commonly used concentrations for IEF are 2-4% (v/v).